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Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer

AIM: This study aimed to evaluate the expression levels of miR-99b and miR-135b in peritoneal carcinoma and liver metastases associated with Colorectal Cancer (CRC), assess their association with the intracellular signaling pathway proteins Kirsten Rat Sarcoma Virus (KRAS) and Akt, and investigate t...

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Autores principales: Aziret, Mehmet, Eskiler, Gamze, Çakar, Gözde Çakırsoy, Ercan, Metin, Bilir, Cemil, Polat, Erdal, Koçer, Havva Belma, Yıldırım, Ebru Kayra, Duman, Mustafa, Özkan, Asuman Deveci
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470417/
https://www.ncbi.nlm.nih.gov/pubmed/37639911
http://dx.doi.org/10.1016/j.clinsp.2023.100271
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author Aziret, Mehmet
Eskiler, Gamze
Çakar, Gözde Çakırsoy
Ercan, Metin
Bilir, Cemil
Polat, Erdal
Koçer, Havva Belma
Yıldırım, Ebru Kayra
Duman, Mustafa
Özkan, Asuman Deveci
author_facet Aziret, Mehmet
Eskiler, Gamze
Çakar, Gözde Çakırsoy
Ercan, Metin
Bilir, Cemil
Polat, Erdal
Koçer, Havva Belma
Yıldırım, Ebru Kayra
Duman, Mustafa
Özkan, Asuman Deveci
author_sort Aziret, Mehmet
collection PubMed
description AIM: This study aimed to evaluate the expression levels of miR-99b and miR-135b in peritoneal carcinoma and liver metastases associated with Colorectal Cancer (CRC), assess their association with the intracellular signaling pathway proteins Kirsten Rat Sarcoma Virus (KRAS) and Akt, and investigate their effects on survival. MATERIALS AND METHODS: Changes in the KRAS gene and Akt proteins, expression levels of miR-99b and miR-135b, and factors affecting survival were compared between colorectal cancer-associated peritoneal carcinomatosis and liver metastasis. RESULTS: The expression levels of miR-99b and miR-135b and the immunohistochemical grade classification score of Akt were higher in colorectal cancer, peritoneal carcinomatosis, and liver metastasis than in normal tissues (p < 0.05). MiR-99b expression was highest in CRC, whereas miR-135b expression was highest in peritoneal carcinomatosis (p < 0.05). The expression level of miR-99b decreased and that of miR-135b increased in peritoneal and liver metastases compared with that in the tumor tissue. MiR-99b, Akt, and recurrence were risk factors that affected the overall survival rate in the model of clinical predictions (p = 0.045, p = 0.006, and p = 0.012, respectively). CONCLUSION: While the expression of miR-99b was highest in the primary tumor, its decrease in liver metastasis and peritoneal carcinomatosis suggests that miR-99b has a protective effect against liver metastasis and peritoneal carcinomatosis. However, the detection of miR-135b expression was highest in peritoneal carcinomatosis and liver metastasis compared with that in the colorectal cancer tissues suggesting that it facilitates peritoneal carcinomatosis and liver metastasis. Furthermore, miR-99b, KRAS mutations, and Akt are risk factors for the overall survival of colorectal cancer.
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spelling pubmed-104704172023-09-01 Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer Aziret, Mehmet Eskiler, Gamze Çakar, Gözde Çakırsoy Ercan, Metin Bilir, Cemil Polat, Erdal Koçer, Havva Belma Yıldırım, Ebru Kayra Duman, Mustafa Özkan, Asuman Deveci Clinics (Sao Paulo) Original Articles AIM: This study aimed to evaluate the expression levels of miR-99b and miR-135b in peritoneal carcinoma and liver metastases associated with Colorectal Cancer (CRC), assess their association with the intracellular signaling pathway proteins Kirsten Rat Sarcoma Virus (KRAS) and Akt, and investigate their effects on survival. MATERIALS AND METHODS: Changes in the KRAS gene and Akt proteins, expression levels of miR-99b and miR-135b, and factors affecting survival were compared between colorectal cancer-associated peritoneal carcinomatosis and liver metastasis. RESULTS: The expression levels of miR-99b and miR-135b and the immunohistochemical grade classification score of Akt were higher in colorectal cancer, peritoneal carcinomatosis, and liver metastasis than in normal tissues (p < 0.05). MiR-99b expression was highest in CRC, whereas miR-135b expression was highest in peritoneal carcinomatosis (p < 0.05). The expression level of miR-99b decreased and that of miR-135b increased in peritoneal and liver metastases compared with that in the tumor tissue. MiR-99b, Akt, and recurrence were risk factors that affected the overall survival rate in the model of clinical predictions (p = 0.045, p = 0.006, and p = 0.012, respectively). CONCLUSION: While the expression of miR-99b was highest in the primary tumor, its decrease in liver metastasis and peritoneal carcinomatosis suggests that miR-99b has a protective effect against liver metastasis and peritoneal carcinomatosis. However, the detection of miR-135b expression was highest in peritoneal carcinomatosis and liver metastasis compared with that in the colorectal cancer tissues suggesting that it facilitates peritoneal carcinomatosis and liver metastasis. Furthermore, miR-99b, KRAS mutations, and Akt are risk factors for the overall survival of colorectal cancer. Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 2023-08-26 /pmc/articles/PMC10470417/ /pubmed/37639911 http://dx.doi.org/10.1016/j.clinsp.2023.100271 Text en © 2023 HCFMUSP. Published by Elsevier España, S.L.U. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Articles
Aziret, Mehmet
Eskiler, Gamze
Çakar, Gözde Çakırsoy
Ercan, Metin
Bilir, Cemil
Polat, Erdal
Koçer, Havva Belma
Yıldırım, Ebru Kayra
Duman, Mustafa
Özkan, Asuman Deveci
Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer
title Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer
title_full Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer
title_fullStr Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer
title_full_unstemmed Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer
title_short Effect of the MiR-99b and MiR-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer
title_sort effect of the mir-99b and mir-135b on peritoneal carcinomatosis and liver metastasis in colorectal cancer
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470417/
https://www.ncbi.nlm.nih.gov/pubmed/37639911
http://dx.doi.org/10.1016/j.clinsp.2023.100271
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