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Nitrogen and sulfur cycling driven by Campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the South China Sea
Chemoautotrophs within Campylobacterota, especially Sulfurovum and Sulfurimonas, are abundant in the seawater–sediment interface of the Formosa cold seep in the South China Sea. However, the in situ activity and function of Campylobacterota are unknown. In this study, the geochemical role of Campylo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Society for Microbiology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470523/ https://www.ncbi.nlm.nih.gov/pubmed/37409803 http://dx.doi.org/10.1128/mbio.00117-23 |
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author | Sun, Qing-lei Xu, Ke Cao, Lei Du, Zengfeng Wang, Minxiao Sun, Li |
author_facet | Sun, Qing-lei Xu, Ke Cao, Lei Du, Zengfeng Wang, Minxiao Sun, Li |
author_sort | Sun, Qing-lei |
collection | PubMed |
description | Chemoautotrophs within Campylobacterota, especially Sulfurovum and Sulfurimonas, are abundant in the seawater–sediment interface of the Formosa cold seep in the South China Sea. However, the in situ activity and function of Campylobacterota are unknown. In this study, the geochemical role of Campylobacterota in the Formosa cold seep was investigated with multiple means. Two members of Sulfurovum and Sulfurimonas were isolated for the first time from deep-sea cold seep. These isolates are new chemoautotrophic species that can use molecular hydrogen as an energy source and CO(2) as a sole carbon source. Comparative genomics identified an important hydrogen-oxidizing cluster in Sulfurovum and Sulfurimonas. Metatranscriptomic analysis detected high expression of hydrogen-oxidizing gene in the RS, suggesting that H(2) was likely an energy source in the cold seep. Genomic analysis indicated that the Sulfurovum and Sulfurimonas isolates possess a truncated sulfur-oxidizing system, and metatranscriptomic analysis revealed that Sulfurovum and Sulfurimonas with this genotype were active in the surface of RS and likely contributed to thiosulfate production. Furthermore, geochemical and in situ analyses revealed sharply decreased nitrate concentration in the sediment–water interface due to microbial consumption. Consistently, the denitrification genes of Sulfurimonas and Sulfurovum were highly expressed, suggesting an important contribution of these bacteria to nitrogen cycling. Overall, this study demonstrated that Campylobacterota played a significant role in the cycling of nitrogen and sulfur in a deep-sea cold seep. IMPORTANCE: Chemoautotrophs within Campylobacterota, in particular Sulfurovum and Sulfurimonas, are ubiquitous in deep-sea cold seeps and hydrothermal vents. However, to date, no Sulfurovum or Sulfurimonas has been isolated from cold seeps, and the ecological roles of these bacteria in cold seeps remain to be investigated. In this study, we obtained two isolates of Sulfurovum and Sulfurimonas from Formosa cold seep, South China Sea. Comparative genomics, metatranscriptomics, geochemical analysis, and in situ experimental study indicated collectively that Campylobacterota played a significant part in nitrogen and sulfur cycling in cold seep and was the cause of thiosulfate accumulation and sharp reduction of nitrate level in the sediment–water interface. The findings of this study promoted our understanding of the in situ function and ecological role of deep-sea Campylobacterota. |
format | Online Article Text |
id | pubmed-10470523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-104705232023-09-01 Nitrogen and sulfur cycling driven by Campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the South China Sea Sun, Qing-lei Xu, Ke Cao, Lei Du, Zengfeng Wang, Minxiao Sun, Li mBio Research Article Chemoautotrophs within Campylobacterota, especially Sulfurovum and Sulfurimonas, are abundant in the seawater–sediment interface of the Formosa cold seep in the South China Sea. However, the in situ activity and function of Campylobacterota are unknown. In this study, the geochemical role of Campylobacterota in the Formosa cold seep was investigated with multiple means. Two members of Sulfurovum and Sulfurimonas were isolated for the first time from deep-sea cold seep. These isolates are new chemoautotrophic species that can use molecular hydrogen as an energy source and CO(2) as a sole carbon source. Comparative genomics identified an important hydrogen-oxidizing cluster in Sulfurovum and Sulfurimonas. Metatranscriptomic analysis detected high expression of hydrogen-oxidizing gene in the RS, suggesting that H(2) was likely an energy source in the cold seep. Genomic analysis indicated that the Sulfurovum and Sulfurimonas isolates possess a truncated sulfur-oxidizing system, and metatranscriptomic analysis revealed that Sulfurovum and Sulfurimonas with this genotype were active in the surface of RS and likely contributed to thiosulfate production. Furthermore, geochemical and in situ analyses revealed sharply decreased nitrate concentration in the sediment–water interface due to microbial consumption. Consistently, the denitrification genes of Sulfurimonas and Sulfurovum were highly expressed, suggesting an important contribution of these bacteria to nitrogen cycling. Overall, this study demonstrated that Campylobacterota played a significant role in the cycling of nitrogen and sulfur in a deep-sea cold seep. IMPORTANCE: Chemoautotrophs within Campylobacterota, in particular Sulfurovum and Sulfurimonas, are ubiquitous in deep-sea cold seeps and hydrothermal vents. However, to date, no Sulfurovum or Sulfurimonas has been isolated from cold seeps, and the ecological roles of these bacteria in cold seeps remain to be investigated. In this study, we obtained two isolates of Sulfurovum and Sulfurimonas from Formosa cold seep, South China Sea. Comparative genomics, metatranscriptomics, geochemical analysis, and in situ experimental study indicated collectively that Campylobacterota played a significant part in nitrogen and sulfur cycling in cold seep and was the cause of thiosulfate accumulation and sharp reduction of nitrate level in the sediment–water interface. The findings of this study promoted our understanding of the in situ function and ecological role of deep-sea Campylobacterota. American Society for Microbiology 2023-07-06 /pmc/articles/PMC10470523/ /pubmed/37409803 http://dx.doi.org/10.1128/mbio.00117-23 Text en Copyright © 2023 Sun et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Sun, Qing-lei Xu, Ke Cao, Lei Du, Zengfeng Wang, Minxiao Sun, Li Nitrogen and sulfur cycling driven by Campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the South China Sea |
title | Nitrogen and sulfur cycling driven by Campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the South China Sea |
title_full | Nitrogen and sulfur cycling driven by Campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the South China Sea |
title_fullStr | Nitrogen and sulfur cycling driven by Campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the South China Sea |
title_full_unstemmed | Nitrogen and sulfur cycling driven by Campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the South China Sea |
title_short | Nitrogen and sulfur cycling driven by Campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the South China Sea |
title_sort | nitrogen and sulfur cycling driven by campylobacterota in the sediment–water interface of deep-sea cold seep: a case in the south china sea |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470523/ https://www.ncbi.nlm.nih.gov/pubmed/37409803 http://dx.doi.org/10.1128/mbio.00117-23 |
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