Cargando…

In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant

Paris polyphylla Smith (Melanthiaceae) family, which is native to the Himalayan region, has received a lot of attention recently due to its extensive history of usage in traditional medicine. The production of steroidal saponin from callus suspension cultures of P. polyphylla was observed in the cur...

Descripción completa

Detalles Bibliográficos
Autores principales: Rawat, Janhvi Mishra, Pandey, Shweta, Rawat, Balwant, Purohit, Sumit, Anand, Jigisha, Negi, Arvind S., Thakur, Ajay, Mahmoud, Mohamed H., El-Gazzar, Ahmed M., El-Saber Batiha, Gaber
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470836/
https://www.ncbi.nlm.nih.gov/pubmed/37662142
http://dx.doi.org/10.3389/fpls.2023.1225612
_version_ 1785099770763673600
author Rawat, Janhvi Mishra
Pandey, Shweta
Rawat, Balwant
Purohit, Sumit
Anand, Jigisha
Negi, Arvind S.
Thakur, Ajay
Mahmoud, Mohamed H.
El-Gazzar, Ahmed M.
El-Saber Batiha, Gaber
author_facet Rawat, Janhvi Mishra
Pandey, Shweta
Rawat, Balwant
Purohit, Sumit
Anand, Jigisha
Negi, Arvind S.
Thakur, Ajay
Mahmoud, Mohamed H.
El-Gazzar, Ahmed M.
El-Saber Batiha, Gaber
author_sort Rawat, Janhvi Mishra
collection PubMed
description Paris polyphylla Smith (Melanthiaceae) family, which is native to the Himalayan region, has received a lot of attention recently due to its extensive history of usage in traditional medicine. The production of steroidal saponin from callus suspension cultures of P. polyphylla was observed in the current study. The current study attempted to develop a P. polyphylla plant callus suspension culture through optimization of cultivation technique for callus suspension, quantification of total phenolic components and estimation of the extract’s antioxidant activity. A light-yellow callus was formed within six weeks of cultivating rhizomes on Murashige and Skoog (MS) media supplemented with Thidiazuron (TDZ). Furthermore, the effect of TDZ, Methyl Jasmonate (MeJA), and Yeast Extract (YE) on callus growth, steroidal saponin (dioscin and diosgenin), total phenolic content, total flavonoids, total tannin, and total antioxidant activity was also measured. The medium containing 0.5 μM TDZ depicted the maximum callus biomass (2.98 g fresh weight). Significantly high phenolic and tannin content was observed in the MS medium containing 50 μM MeJA, whereas, no significant increase was observed in total tannin production in any treatment. Three in vitro assays, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis (3-ethylbenzothiazoline- 6-sulfonic acid)) and FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), were used to assess antioxidant potential of callus. Maximum antioxidant analysis reported in 1.0 μM TDZ (6.89 mM AAE/100 g) containing medium followed by 50 μM MeJA (6.44 mM AAE/100 g). The HPLC analysis showed a high presence of dioscin and diosgenin (5.43% and 21.09%, respectively) compared to the wild sample (2.56% and 15.05%, respectively). According to the results, callus produced on media supplemented with 50 μM MeJA have significant phenolic contents and elevated antioxidant activity; nevertheless, callus growth was greater in the presence of 0.5 μM TDZ. The findings of the current study have commercial implications since greater biomass production will result in active phytochemicals that the pharmaceutical and nutraceutical sectors are in need desperately.
format Online
Article
Text
id pubmed-10470836
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-104708362023-09-01 In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant Rawat, Janhvi Mishra Pandey, Shweta Rawat, Balwant Purohit, Sumit Anand, Jigisha Negi, Arvind S. Thakur, Ajay Mahmoud, Mohamed H. El-Gazzar, Ahmed M. El-Saber Batiha, Gaber Front Plant Sci Plant Science Paris polyphylla Smith (Melanthiaceae) family, which is native to the Himalayan region, has received a lot of attention recently due to its extensive history of usage in traditional medicine. The production of steroidal saponin from callus suspension cultures of P. polyphylla was observed in the current study. The current study attempted to develop a P. polyphylla plant callus suspension culture through optimization of cultivation technique for callus suspension, quantification of total phenolic components and estimation of the extract’s antioxidant activity. A light-yellow callus was formed within six weeks of cultivating rhizomes on Murashige and Skoog (MS) media supplemented with Thidiazuron (TDZ). Furthermore, the effect of TDZ, Methyl Jasmonate (MeJA), and Yeast Extract (YE) on callus growth, steroidal saponin (dioscin and diosgenin), total phenolic content, total flavonoids, total tannin, and total antioxidant activity was also measured. The medium containing 0.5 μM TDZ depicted the maximum callus biomass (2.98 g fresh weight). Significantly high phenolic and tannin content was observed in the MS medium containing 50 μM MeJA, whereas, no significant increase was observed in total tannin production in any treatment. Three in vitro assays, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis (3-ethylbenzothiazoline- 6-sulfonic acid)) and FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), were used to assess antioxidant potential of callus. Maximum antioxidant analysis reported in 1.0 μM TDZ (6.89 mM AAE/100 g) containing medium followed by 50 μM MeJA (6.44 mM AAE/100 g). The HPLC analysis showed a high presence of dioscin and diosgenin (5.43% and 21.09%, respectively) compared to the wild sample (2.56% and 15.05%, respectively). According to the results, callus produced on media supplemented with 50 μM MeJA have significant phenolic contents and elevated antioxidant activity; nevertheless, callus growth was greater in the presence of 0.5 μM TDZ. The findings of the current study have commercial implications since greater biomass production will result in active phytochemicals that the pharmaceutical and nutraceutical sectors are in need desperately. Frontiers Media S.A. 2023-08-10 /pmc/articles/PMC10470836/ /pubmed/37662142 http://dx.doi.org/10.3389/fpls.2023.1225612 Text en Copyright © 2023 Rawat, Pandey, Rawat, Purohit, Anand, Negi, Thakur, Mahmoud, El-Gazzar and El-Saber Batiha https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Rawat, Janhvi Mishra
Pandey, Shweta
Rawat, Balwant
Purohit, Sumit
Anand, Jigisha
Negi, Arvind S.
Thakur, Ajay
Mahmoud, Mohamed H.
El-Gazzar, Ahmed M.
El-Saber Batiha, Gaber
In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant
title In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant
title_full In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant
title_fullStr In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant
title_full_unstemmed In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant
title_short In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant
title_sort in vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of paris polyphylla smith: an important himalayan medicinal plant
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470836/
https://www.ncbi.nlm.nih.gov/pubmed/37662142
http://dx.doi.org/10.3389/fpls.2023.1225612
work_keys_str_mv AT rawatjanhvimishra invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT pandeyshweta invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT rawatbalwant invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT purohitsumit invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT anandjigisha invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT negiarvinds invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT thakurajay invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT mahmoudmohamedh invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT elgazzarahmedm invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant
AT elsaberbatihagaber invitroproductionofsteroidalsaponintotalphenolsandantioxidantactivityincallussuspensioncultureofparispolyphyllasmithanimportanthimalayanmedicinalplant