Cargando…
Morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower
Genetic diversity is the prerequisite for the success of crop improvement programmes. Keeping in view, the current investigation was undertaken to assess the agro-morphological and molecular diversity involving 36 diverse mid-late and late cauliflower genotypes following α-RBD design during winter s...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470947/ https://www.ncbi.nlm.nih.gov/pubmed/37651405 http://dx.doi.org/10.1371/journal.pone.0290495 |
_version_ | 1785099796218904576 |
---|---|
author | Rana, Neha Sharma, Akhilesh Rana, Ranbir Singh Lata, Hem Bansuli Thakur, Alisha Singh, Vivek Sood, Aditya |
author_facet | Rana, Neha Sharma, Akhilesh Rana, Ranbir Singh Lata, Hem Bansuli Thakur, Alisha Singh, Vivek Sood, Aditya |
author_sort | Rana, Neha |
collection | PubMed |
description | Genetic diversity is the prerequisite for the success of crop improvement programmes. Keeping in view, the current investigation was undertaken to assess the agro-morphological and molecular diversity involving 36 diverse mid-late and late cauliflower genotypes following α-RBD design during winter season 2021–22. Six morphological descriptors predicted as polymorphic using Shannon diversity index with maximum for leaf margin (0.94). The genotypes grouped into nine clusters based on D(2) analysis with four as monogenotypic and gross plant weight (32.38%) revealed maximum contribution towards the genetic diversity. Molecular diversity analysis revealed 2–7 alleles among 36 polymorphic simple sequence repeats (SSR) with average of 4.22. Primer BoESSR492 (0.77) showed maximum polymorphic information content (PIC) with mean of 0.58. SSR analysis revealed two clusters each with two subclusters with a composite pattern of genotype distribution. STRUCTURE analysis showed homogenous mixture with least amount of gene pool introgression within the genotypes. Thus, based on morphological and molecular studies, the diverse genotypes namely, DPCaCMS-1, DPCaf-W4, DPCaf-US, DPCaf-W131W, DPCaf-S121, DPCaf-18, DPCaf-13, DPCaf-29 and DPCaf-CMS5 can be utilized in hybridization to isolate potential transgressive segregants to broaden the genetic base of cauliflower or involve them to exploit heterosis. |
format | Online Article Text |
id | pubmed-10470947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104709472023-09-01 Morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower Rana, Neha Sharma, Akhilesh Rana, Ranbir Singh Lata, Hem Bansuli Thakur, Alisha Singh, Vivek Sood, Aditya PLoS One Research Article Genetic diversity is the prerequisite for the success of crop improvement programmes. Keeping in view, the current investigation was undertaken to assess the agro-morphological and molecular diversity involving 36 diverse mid-late and late cauliflower genotypes following α-RBD design during winter season 2021–22. Six morphological descriptors predicted as polymorphic using Shannon diversity index with maximum for leaf margin (0.94). The genotypes grouped into nine clusters based on D(2) analysis with four as monogenotypic and gross plant weight (32.38%) revealed maximum contribution towards the genetic diversity. Molecular diversity analysis revealed 2–7 alleles among 36 polymorphic simple sequence repeats (SSR) with average of 4.22. Primer BoESSR492 (0.77) showed maximum polymorphic information content (PIC) with mean of 0.58. SSR analysis revealed two clusters each with two subclusters with a composite pattern of genotype distribution. STRUCTURE analysis showed homogenous mixture with least amount of gene pool introgression within the genotypes. Thus, based on morphological and molecular studies, the diverse genotypes namely, DPCaCMS-1, DPCaf-W4, DPCaf-US, DPCaf-W131W, DPCaf-S121, DPCaf-18, DPCaf-13, DPCaf-29 and DPCaf-CMS5 can be utilized in hybridization to isolate potential transgressive segregants to broaden the genetic base of cauliflower or involve them to exploit heterosis. Public Library of Science 2023-08-31 /pmc/articles/PMC10470947/ /pubmed/37651405 http://dx.doi.org/10.1371/journal.pone.0290495 Text en © 2023 Rana et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rana, Neha Sharma, Akhilesh Rana, Ranbir Singh Lata, Hem Bansuli Thakur, Alisha Singh, Vivek Sood, Aditya Morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower |
title | Morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower |
title_full | Morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower |
title_fullStr | Morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower |
title_full_unstemmed | Morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower |
title_short | Morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower |
title_sort | morphological and molecular diversity in mid-late and late maturity genotypes of cauliflower |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470947/ https://www.ncbi.nlm.nih.gov/pubmed/37651405 http://dx.doi.org/10.1371/journal.pone.0290495 |
work_keys_str_mv | AT rananeha morphologicalandmoleculardiversityinmidlateandlatematuritygenotypesofcauliflower AT sharmaakhilesh morphologicalandmoleculardiversityinmidlateandlatematuritygenotypesofcauliflower AT ranaranbirsingh morphologicalandmoleculardiversityinmidlateandlatematuritygenotypesofcauliflower AT latahem morphologicalandmoleculardiversityinmidlateandlatematuritygenotypesofcauliflower AT bansuli morphologicalandmoleculardiversityinmidlateandlatematuritygenotypesofcauliflower AT thakuralisha morphologicalandmoleculardiversityinmidlateandlatematuritygenotypesofcauliflower AT singhvivek morphologicalandmoleculardiversityinmidlateandlatematuritygenotypesofcauliflower AT soodaditya morphologicalandmoleculardiversityinmidlateandlatematuritygenotypesofcauliflower |