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Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera)

The practice of artificial insemination for the long-tailed chinchilla has not been fully elaborated to date, and existing data available regarding their reproduction properties is contradictory. Until now, the collection of semen for chinchillas has been most-commonly obtained using electro-ejacula...

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Autores principales: Babarczi, Bianka, Drobnyák, Árpád, Barna, Judit, Kissné, Éva Váradi, Szabó, Zsuzsa, Heincinger, Mónika, Kustos, Károly, Szőke, Zsuzsanna, Végi, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10471009/
https://www.ncbi.nlm.nih.gov/pubmed/37651370
http://dx.doi.org/10.1371/journal.pone.0290441
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author Babarczi, Bianka
Drobnyák, Árpád
Barna, Judit
Kissné, Éva Váradi
Szabó, Zsuzsa
Heincinger, Mónika
Kustos, Károly
Szőke, Zsuzsanna
Végi, Barbara
author_facet Babarczi, Bianka
Drobnyák, Árpád
Barna, Judit
Kissné, Éva Váradi
Szabó, Zsuzsa
Heincinger, Mónika
Kustos, Károly
Szőke, Zsuzsanna
Végi, Barbara
author_sort Babarczi, Bianka
collection PubMed
description The practice of artificial insemination for the long-tailed chinchilla has not been fully elaborated to date, and existing data available regarding their reproduction properties is contradictory. Until now, the collection of semen for chinchillas has been most-commonly obtained using electro-ejaculation methods exclusively. The primary objective of this study was the development of a manual technique for semen collection which meets all animal welfare requirements. An additional aim was to determine the basic spermatological parameters, such as motility, concentration, type and ratio of morphological abnormalities and live/dead cell ratio, under typical northern-hemisphere conditions, in Hungary. Over a 3 month period, a special massage technique was developed for the study, and using this method, the sperm parameters of 46 males were subsequently analyzed weekly for a period of one year. Approximately 66% of chinchillas responded positively to this technique, with the success rate of semen-collection attempts showing no variation between seasons. Average sperm concentration for the whole year was 935.17 million/ml using this method. Total cell motility was the highest in winter (90.3%), and the lowest in spring (84.3%). The proportion of live, intact cells were above 80% on average for the year, while the ratios of live, morphologically abnormal and dead cells were 6% and 14%, respectively. We found that midpiece abnormalities occurred in the highest proportion (0.95%-3.38%), while the head abnormalities showed the lowest ratio (0.01%-0.15%). Standard deviation among the parameters was relatively high, with the spring season proving to be the weakest in terms of sperm quality. This study has demonstrated that, semen can be successfully collected without the use of electro-ejaculation or anesthesia. Furthermore, although spermatological parameters do exhibit some fluctuation for the different times of the year, semen collected is nonetheless suitable for the purpose of artificial insemination of chinchillas at any time.
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spelling pubmed-104710092023-09-01 Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera) Babarczi, Bianka Drobnyák, Árpád Barna, Judit Kissné, Éva Váradi Szabó, Zsuzsa Heincinger, Mónika Kustos, Károly Szőke, Zsuzsanna Végi, Barbara PLoS One Research Article The practice of artificial insemination for the long-tailed chinchilla has not been fully elaborated to date, and existing data available regarding their reproduction properties is contradictory. Until now, the collection of semen for chinchillas has been most-commonly obtained using electro-ejaculation methods exclusively. The primary objective of this study was the development of a manual technique for semen collection which meets all animal welfare requirements. An additional aim was to determine the basic spermatological parameters, such as motility, concentration, type and ratio of morphological abnormalities and live/dead cell ratio, under typical northern-hemisphere conditions, in Hungary. Over a 3 month period, a special massage technique was developed for the study, and using this method, the sperm parameters of 46 males were subsequently analyzed weekly for a period of one year. Approximately 66% of chinchillas responded positively to this technique, with the success rate of semen-collection attempts showing no variation between seasons. Average sperm concentration for the whole year was 935.17 million/ml using this method. Total cell motility was the highest in winter (90.3%), and the lowest in spring (84.3%). The proportion of live, intact cells were above 80% on average for the year, while the ratios of live, morphologically abnormal and dead cells were 6% and 14%, respectively. We found that midpiece abnormalities occurred in the highest proportion (0.95%-3.38%), while the head abnormalities showed the lowest ratio (0.01%-0.15%). Standard deviation among the parameters was relatively high, with the spring season proving to be the weakest in terms of sperm quality. This study has demonstrated that, semen can be successfully collected without the use of electro-ejaculation or anesthesia. Furthermore, although spermatological parameters do exhibit some fluctuation for the different times of the year, semen collected is nonetheless suitable for the purpose of artificial insemination of chinchillas at any time. Public Library of Science 2023-08-31 /pmc/articles/PMC10471009/ /pubmed/37651370 http://dx.doi.org/10.1371/journal.pone.0290441 Text en © 2023 Babarczi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Babarczi, Bianka
Drobnyák, Árpád
Barna, Judit
Kissné, Éva Váradi
Szabó, Zsuzsa
Heincinger, Mónika
Kustos, Károly
Szőke, Zsuzsanna
Végi, Barbara
Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera)
title Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera)
title_full Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera)
title_fullStr Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera)
title_full_unstemmed Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera)
title_short Elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (Chinchilla lanigera)
title_sort elaboration of massage technique for semen collection and examination of semen characteristics in chinchilla (chinchilla lanigera)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10471009/
https://www.ncbi.nlm.nih.gov/pubmed/37651370
http://dx.doi.org/10.1371/journal.pone.0290441
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