Cargando…

Composition and functional diversity of bacterial communities during swine carcass decomposition

OBJECTIVE: This study investigated the changes in bacterial communities within decomposing swine microcosms, comparing soil with or without intact microbial communities, and under aerobic and anaerobic conditions. METHODS: The experimental microcosms consisted of four conditions: UA, unsterilized so...

Descripción completa

Detalles Bibliográficos
Autores principales: Miguel, Michelle, Kim, Seon-Ho, Lee, Sang-Suk, Cho, Yong-Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Animal Bioscience 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472150/
https://www.ncbi.nlm.nih.gov/pubmed/37402447
http://dx.doi.org/10.5713/ab.23.0140
_version_ 1785100013434568704
author Miguel, Michelle
Kim, Seon-Ho
Lee, Sang-Suk
Cho, Yong-Il
author_facet Miguel, Michelle
Kim, Seon-Ho
Lee, Sang-Suk
Cho, Yong-Il
author_sort Miguel, Michelle
collection PubMed
description OBJECTIVE: This study investigated the changes in bacterial communities within decomposing swine microcosms, comparing soil with or without intact microbial communities, and under aerobic and anaerobic conditions. METHODS: The experimental microcosms consisted of four conditions: UA, unsterilized soil–aerobic condition; SA, sterilized soil–aerobic condition; UAn, unsterilized soil–anaerobic condition; and San, sterilized soil–anaerobic condition. The microcosms were prepared by mixing 112.5 g of soil and 37.5 g of ground carcass, which were then placed in sterile containers. The carcass-soil mixture was sampled at day 0, 5, 10, 30, and 60 of decomposition, and the bacterial communities that formed during carcass decomposition were assessed using Illumina MiSeq sequencing of the 16S rRNA gene. RESULTS: A total of 1,687 amplicon sequence variants representing 22 phyla and 805 genera were identified in the microcosms. The Chao1 and Shannon diversity indices varied in between microcosms at each period (p<0.05). Metagenomic analysis showed variation in the taxa composition across the burial microcosms during decomposition, with Firmicutes being the dominant phylum, followed by Proteobacteria. At the genus level, Bacillus and Clostridium were the main genera within Firmicutes. Functional prediction revealed that the most abundant Kyoto encyclopedia of genes and genomes metabolic functions were carbohydrate and amino acid metabolisms. CONCLUSION: This study demonstrated a higher bacteria diversity in UA and UAn microcosms than in SA and SAn microcosms. In addition, the taxonomic composition of the microbial community also exhibited changes, highlighting the impact of soil sterilization and oxygen on carcass decomposition. Furthermore, this study provided insights into the microbial communities associated with decomposing swine carcasses in microcosm.
format Online
Article
Text
id pubmed-10472150
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Animal Bioscience
record_format MEDLINE/PubMed
spelling pubmed-104721502023-09-02 Composition and functional diversity of bacterial communities during swine carcass decomposition Miguel, Michelle Kim, Seon-Ho Lee, Sang-Suk Cho, Yong-Il Anim Biosci Article OBJECTIVE: This study investigated the changes in bacterial communities within decomposing swine microcosms, comparing soil with or without intact microbial communities, and under aerobic and anaerobic conditions. METHODS: The experimental microcosms consisted of four conditions: UA, unsterilized soil–aerobic condition; SA, sterilized soil–aerobic condition; UAn, unsterilized soil–anaerobic condition; and San, sterilized soil–anaerobic condition. The microcosms were prepared by mixing 112.5 g of soil and 37.5 g of ground carcass, which were then placed in sterile containers. The carcass-soil mixture was sampled at day 0, 5, 10, 30, and 60 of decomposition, and the bacterial communities that formed during carcass decomposition were assessed using Illumina MiSeq sequencing of the 16S rRNA gene. RESULTS: A total of 1,687 amplicon sequence variants representing 22 phyla and 805 genera were identified in the microcosms. The Chao1 and Shannon diversity indices varied in between microcosms at each period (p<0.05). Metagenomic analysis showed variation in the taxa composition across the burial microcosms during decomposition, with Firmicutes being the dominant phylum, followed by Proteobacteria. At the genus level, Bacillus and Clostridium were the main genera within Firmicutes. Functional prediction revealed that the most abundant Kyoto encyclopedia of genes and genomes metabolic functions were carbohydrate and amino acid metabolisms. CONCLUSION: This study demonstrated a higher bacteria diversity in UA and UAn microcosms than in SA and SAn microcosms. In addition, the taxonomic composition of the microbial community also exhibited changes, highlighting the impact of soil sterilization and oxygen on carcass decomposition. Furthermore, this study provided insights into the microbial communities associated with decomposing swine carcasses in microcosm. Animal Bioscience 2023-09 2023-06-26 /pmc/articles/PMC10472150/ /pubmed/37402447 http://dx.doi.org/10.5713/ab.23.0140 Text en Copyright © 2023 by Animal Bioscience https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Miguel, Michelle
Kim, Seon-Ho
Lee, Sang-Suk
Cho, Yong-Il
Composition and functional diversity of bacterial communities during swine carcass decomposition
title Composition and functional diversity of bacterial communities during swine carcass decomposition
title_full Composition and functional diversity of bacterial communities during swine carcass decomposition
title_fullStr Composition and functional diversity of bacterial communities during swine carcass decomposition
title_full_unstemmed Composition and functional diversity of bacterial communities during swine carcass decomposition
title_short Composition and functional diversity of bacterial communities during swine carcass decomposition
title_sort composition and functional diversity of bacterial communities during swine carcass decomposition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472150/
https://www.ncbi.nlm.nih.gov/pubmed/37402447
http://dx.doi.org/10.5713/ab.23.0140
work_keys_str_mv AT miguelmichelle compositionandfunctionaldiversityofbacterialcommunitiesduringswinecarcassdecomposition
AT kimseonho compositionandfunctionaldiversityofbacterialcommunitiesduringswinecarcassdecomposition
AT leesangsuk compositionandfunctionaldiversityofbacterialcommunitiesduringswinecarcassdecomposition
AT choyongil compositionandfunctionaldiversityofbacterialcommunitiesduringswinecarcassdecomposition