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Ictal vocalizations in the Scn1a (+/−) mouse model of Dravet syndrome

OBJECTIVE: Ictal vocalizations have shown diagnostic utility in epilepsy patients. Audio recordings of seizures have also been used for seizure detection. The present study aimed to determine whether generalized tonic–clonic seizures in the Scn1a (+/−) mouse model of Dravet syndrome are associated w...

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Autores principales: Anderson, Lyndsey L., Everett‐Morgan, Declan, Petkova, Stela P., Silverman, Jill L., Arnold, Jonathon C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472354/
https://www.ncbi.nlm.nih.gov/pubmed/36811143
http://dx.doi.org/10.1002/epi4.12715
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author Anderson, Lyndsey L.
Everett‐Morgan, Declan
Petkova, Stela P.
Silverman, Jill L.
Arnold, Jonathon C.
author_facet Anderson, Lyndsey L.
Everett‐Morgan, Declan
Petkova, Stela P.
Silverman, Jill L.
Arnold, Jonathon C.
author_sort Anderson, Lyndsey L.
collection PubMed
description OBJECTIVE: Ictal vocalizations have shown diagnostic utility in epilepsy patients. Audio recordings of seizures have also been used for seizure detection. The present study aimed to determine whether generalized tonic–clonic seizures in the Scn1a (+/−) mouse model of Dravet syndrome are associated with either audible mouse squeaks or ultrasonic vocalizations. METHODS: Acoustic recordings were captured from group‐housed Scn1a (+/−) mice undergoing video‐monitoring to quantify spontaneous seizure frequency. We generated audio clips (n = 129) during a generalized tonic–clonic seizure (GTCS) that included 30 seconds immediately prior to the GTCS (preictal) and 30 seconds following the conclusion of the seizure (postictal). Nonseizure clips (n = 129) were also exported from the acoustic recordings. A blinded reviewer manually reviewed the audio clips, and vocalizations were identified as either an audible (<20 kHz) mouse squeak or ultrasonic (>20 kHz). RESULTS: Spontaneous GTCS in Scn1a (+/−) mice were associated with a significantly higher number of total vocalizations. The number of audible mouse squeaks was significantly greater with GTCS activity. Nearly all (98%) the seizure clips contained ultrasonic vocalizations, whereas ultrasonic vocalizations were present in only 57% of nonseizure clips. The ultrasonic vocalizations emitted in the seizure clips were at a significantly higher frequency and were nearly twice as long in duration as those emitted in the nonseizure clips. Audible mouse squeaks were primarily emitted during the preictal phase. The greatest number of ultrasonic vocalizations was detected during the ictal phase. SIGNIFICANCE: Our study shows that ictal vocalizations are exhibited by Scn1a (+/−) mice. Quantitative audio analysis could be developed as a seizure detection tool for the Scn1a (+/−) mouse model of Dravet syndrome.
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spelling pubmed-104723542023-09-02 Ictal vocalizations in the Scn1a (+/−) mouse model of Dravet syndrome Anderson, Lyndsey L. Everett‐Morgan, Declan Petkova, Stela P. Silverman, Jill L. Arnold, Jonathon C. Epilepsia Open Original Articles OBJECTIVE: Ictal vocalizations have shown diagnostic utility in epilepsy patients. Audio recordings of seizures have also been used for seizure detection. The present study aimed to determine whether generalized tonic–clonic seizures in the Scn1a (+/−) mouse model of Dravet syndrome are associated with either audible mouse squeaks or ultrasonic vocalizations. METHODS: Acoustic recordings were captured from group‐housed Scn1a (+/−) mice undergoing video‐monitoring to quantify spontaneous seizure frequency. We generated audio clips (n = 129) during a generalized tonic–clonic seizure (GTCS) that included 30 seconds immediately prior to the GTCS (preictal) and 30 seconds following the conclusion of the seizure (postictal). Nonseizure clips (n = 129) were also exported from the acoustic recordings. A blinded reviewer manually reviewed the audio clips, and vocalizations were identified as either an audible (<20 kHz) mouse squeak or ultrasonic (>20 kHz). RESULTS: Spontaneous GTCS in Scn1a (+/−) mice were associated with a significantly higher number of total vocalizations. The number of audible mouse squeaks was significantly greater with GTCS activity. Nearly all (98%) the seizure clips contained ultrasonic vocalizations, whereas ultrasonic vocalizations were present in only 57% of nonseizure clips. The ultrasonic vocalizations emitted in the seizure clips were at a significantly higher frequency and were nearly twice as long in duration as those emitted in the nonseizure clips. Audible mouse squeaks were primarily emitted during the preictal phase. The greatest number of ultrasonic vocalizations was detected during the ictal phase. SIGNIFICANCE: Our study shows that ictal vocalizations are exhibited by Scn1a (+/−) mice. Quantitative audio analysis could be developed as a seizure detection tool for the Scn1a (+/−) mouse model of Dravet syndrome. John Wiley and Sons Inc. 2023-05-15 /pmc/articles/PMC10472354/ /pubmed/36811143 http://dx.doi.org/10.1002/epi4.12715 Text en © 2023 The Authors. Epilepsia Open published by Wiley Periodicals LLC on behalf of International League Against Epilepsy. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Anderson, Lyndsey L.
Everett‐Morgan, Declan
Petkova, Stela P.
Silverman, Jill L.
Arnold, Jonathon C.
Ictal vocalizations in the Scn1a (+/−) mouse model of Dravet syndrome
title Ictal vocalizations in the Scn1a (+/−) mouse model of Dravet syndrome
title_full Ictal vocalizations in the Scn1a (+/−) mouse model of Dravet syndrome
title_fullStr Ictal vocalizations in the Scn1a (+/−) mouse model of Dravet syndrome
title_full_unstemmed Ictal vocalizations in the Scn1a (+/−) mouse model of Dravet syndrome
title_short Ictal vocalizations in the Scn1a (+/−) mouse model of Dravet syndrome
title_sort ictal vocalizations in the scn1a (+/−) mouse model of dravet syndrome
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472354/
https://www.ncbi.nlm.nih.gov/pubmed/36811143
http://dx.doi.org/10.1002/epi4.12715
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