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Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids

BACKGROUND: The corpus cavernosum (CC) containing sinusoids plays fundamental roles for erection. Analysis of pathological changes in the erectile system is studied by recent experimental systems. Various in vitro models utilizing genital mesenchymal‐derived cells and explant culture systems are sum...

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Autores principales: Hashimoto, Daiki, Fujimoto, Kota, Kim, Sang Woon, Lee, Yong Seung, Nakata, Masanori, Suzuki, Kentaro, Wada, Yoshitaka, Asamura, Shinichi, Yamada, Gen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472535/
https://www.ncbi.nlm.nih.gov/pubmed/37663955
http://dx.doi.org/10.1002/rmb2.12539
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author Hashimoto, Daiki
Fujimoto, Kota
Kim, Sang Woon
Lee, Yong Seung
Nakata, Masanori
Suzuki, Kentaro
Wada, Yoshitaka
Asamura, Shinichi
Yamada, Gen
author_facet Hashimoto, Daiki
Fujimoto, Kota
Kim, Sang Woon
Lee, Yong Seung
Nakata, Masanori
Suzuki, Kentaro
Wada, Yoshitaka
Asamura, Shinichi
Yamada, Gen
author_sort Hashimoto, Daiki
collection PubMed
description BACKGROUND: The corpus cavernosum (CC) containing sinusoids plays fundamental roles for erection. Analysis of pathological changes in the erectile system is studied by recent experimental systems. Various in vitro models utilizing genital mesenchymal‐derived cells and explant culture systems are summarized. METHODS: 3D reconstruction of section images of murine CC was created. Ectopic chondrogenesis in aged mouse CC was shown by a gene expression study revealing the prominent expression of Sox9. Various experimental strategies utilizing mesenchyme‐derived primary cells and tissue explants are introduced. MAIN FINDINGS: Possible roles of Sox9 in chondrogenesis and its regulation by several signals are suggested. The unique character of genital mesenchyme is shown by various analyses of external genitalia (ExG) derived cells and explant cultures. Such strategies are also applied to the analysis of erectile contraction/relaxation responses to many signals and aging process. CONCLUSION: Erectile dysfunction (ED) is one of the essential topics for the modern aged society. More comprehensive studies are necessary to reveal the nature of the erectile system by combining multiple cell culture strategies.
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spelling pubmed-104725352023-09-02 Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids Hashimoto, Daiki Fujimoto, Kota Kim, Sang Woon Lee, Yong Seung Nakata, Masanori Suzuki, Kentaro Wada, Yoshitaka Asamura, Shinichi Yamada, Gen Reprod Med Biol Reviews BACKGROUND: The corpus cavernosum (CC) containing sinusoids plays fundamental roles for erection. Analysis of pathological changes in the erectile system is studied by recent experimental systems. Various in vitro models utilizing genital mesenchymal‐derived cells and explant culture systems are summarized. METHODS: 3D reconstruction of section images of murine CC was created. Ectopic chondrogenesis in aged mouse CC was shown by a gene expression study revealing the prominent expression of Sox9. Various experimental strategies utilizing mesenchyme‐derived primary cells and tissue explants are introduced. MAIN FINDINGS: Possible roles of Sox9 in chondrogenesis and its regulation by several signals are suggested. The unique character of genital mesenchyme is shown by various analyses of external genitalia (ExG) derived cells and explant cultures. Such strategies are also applied to the analysis of erectile contraction/relaxation responses to many signals and aging process. CONCLUSION: Erectile dysfunction (ED) is one of the essential topics for the modern aged society. More comprehensive studies are necessary to reveal the nature of the erectile system by combining multiple cell culture strategies. John Wiley and Sons Inc. 2023-09-01 /pmc/articles/PMC10472535/ /pubmed/37663955 http://dx.doi.org/10.1002/rmb2.12539 Text en © 2023 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Reviews
Hashimoto, Daiki
Fujimoto, Kota
Kim, Sang Woon
Lee, Yong Seung
Nakata, Masanori
Suzuki, Kentaro
Wada, Yoshitaka
Asamura, Shinichi
Yamada, Gen
Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids
title Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids
title_full Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids
title_fullStr Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids
title_full_unstemmed Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids
title_short Emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids
title_sort emerging structural and pathological analyses on the erectile organ, corpus cavernous containing sinusoids
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472535/
https://www.ncbi.nlm.nih.gov/pubmed/37663955
http://dx.doi.org/10.1002/rmb2.12539
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