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Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions

BACKGROUND: Intestinal health plays a pivotal role in broiler chicken growth. Oregano aqueous extract (OAE) effectively exerts anti-inflammatory and antibacterial effects. However, the protective effects of OAE on intestinal health in broilers and the underlying mechanism remain unclear. This study...

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Autores principales: Zhang, Fan, Yang, Jiantao, Zhan, Qinyi, Shi, Hao, Li, Yanhe, Li, Dinggang, Li, Yingge, Yang, Xiaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472629/
https://www.ncbi.nlm.nih.gov/pubmed/37653529
http://dx.doi.org/10.1186/s40104-023-00857-w
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author Zhang, Fan
Yang, Jiantao
Zhan, Qinyi
Shi, Hao
Li, Yanhe
Li, Dinggang
Li, Yingge
Yang, Xiaojun
author_facet Zhang, Fan
Yang, Jiantao
Zhan, Qinyi
Shi, Hao
Li, Yanhe
Li, Dinggang
Li, Yingge
Yang, Xiaojun
author_sort Zhang, Fan
collection PubMed
description BACKGROUND: Intestinal health plays a pivotal role in broiler chicken growth. Oregano aqueous extract (OAE) effectively exerts anti-inflammatory and antibacterial effects. However, the protective effects of OAE on intestinal health in broilers and the underlying mechanism remain unclear. This study aimed to investigate the potential effects of OAE on growth performance, the gut microbiota and intestinal health. A total of 840 1-d-old male and female broilers (Arbor Acres) were randomly allocated into 6 groups as follows: basal diet (Con), Con + antibiotics (Anti, colistin sulfate 7 g/kg, roxarsone 35 g/kg), Con + 400, 500, 600 and 700 mg/kg OAE (OAE400, OAE500, OAE600 and OAE700). Subsequently, fermentation in vitro together with oral administration trials were carried out to further assess the function of OAE on intestinal health of broilers. RESULTS: Dietary 700 mg/kg OAE supplementation resulted in an increase (P < 0.05) in body weight and a decrease (P < 0.05) in feed conversion ratio when compared with the control during d 22 to 42 of the trial. OAE addition resulted in lower (P < 0.05) jejunal crypt depth and mRNA expression of IL-4 and IL-10 at d 42. In addition, dietary OAE addition increased the abundance of Firmicutes (P = 0.087) and Lactobacillus (P < 0.05) in the cecum, and increased (P < 0.05) the content of acetic acid and butyric acid. In the in vitro fermentation test, OAE significantly increased (P < 0.05) the abundance of Lactobacillus, decreased (P < 0.05) the abundance of unspecified_Enterobacteriaceae, and increased the content of acetic acid (P < 0.05). In the oral administration trial, higher (P < 0.05) IL-4 expression was found in broilers when oral inoculation with oregano fermentation microorganisms at d 42. And SIgA content in the ileum was significantly increased (P = 0.073) when giving OAE fermentation supernatant. CONCLUSIONS: Dietary OAE addition could maintain intestinal health and improve growth performance through enhancing intestinal mucosal immunity and barrier function mediated by gut microbiota changes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-023-00857-w.
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spelling pubmed-104726292023-09-02 Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions Zhang, Fan Yang, Jiantao Zhan, Qinyi Shi, Hao Li, Yanhe Li, Dinggang Li, Yingge Yang, Xiaojun J Anim Sci Biotechnol Research BACKGROUND: Intestinal health plays a pivotal role in broiler chicken growth. Oregano aqueous extract (OAE) effectively exerts anti-inflammatory and antibacterial effects. However, the protective effects of OAE on intestinal health in broilers and the underlying mechanism remain unclear. This study aimed to investigate the potential effects of OAE on growth performance, the gut microbiota and intestinal health. A total of 840 1-d-old male and female broilers (Arbor Acres) were randomly allocated into 6 groups as follows: basal diet (Con), Con + antibiotics (Anti, colistin sulfate 7 g/kg, roxarsone 35 g/kg), Con + 400, 500, 600 and 700 mg/kg OAE (OAE400, OAE500, OAE600 and OAE700). Subsequently, fermentation in vitro together with oral administration trials were carried out to further assess the function of OAE on intestinal health of broilers. RESULTS: Dietary 700 mg/kg OAE supplementation resulted in an increase (P < 0.05) in body weight and a decrease (P < 0.05) in feed conversion ratio when compared with the control during d 22 to 42 of the trial. OAE addition resulted in lower (P < 0.05) jejunal crypt depth and mRNA expression of IL-4 and IL-10 at d 42. In addition, dietary OAE addition increased the abundance of Firmicutes (P = 0.087) and Lactobacillus (P < 0.05) in the cecum, and increased (P < 0.05) the content of acetic acid and butyric acid. In the in vitro fermentation test, OAE significantly increased (P < 0.05) the abundance of Lactobacillus, decreased (P < 0.05) the abundance of unspecified_Enterobacteriaceae, and increased the content of acetic acid (P < 0.05). In the oral administration trial, higher (P < 0.05) IL-4 expression was found in broilers when oral inoculation with oregano fermentation microorganisms at d 42. And SIgA content in the ileum was significantly increased (P = 0.073) when giving OAE fermentation supernatant. CONCLUSIONS: Dietary OAE addition could maintain intestinal health and improve growth performance through enhancing intestinal mucosal immunity and barrier function mediated by gut microbiota changes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-023-00857-w. BioMed Central 2023-09-01 /pmc/articles/PMC10472629/ /pubmed/37653529 http://dx.doi.org/10.1186/s40104-023-00857-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Fan
Yang, Jiantao
Zhan, Qinyi
Shi, Hao
Li, Yanhe
Li, Dinggang
Li, Yingge
Yang, Xiaojun
Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions
title Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions
title_full Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions
title_fullStr Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions
title_full_unstemmed Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions
title_short Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions
title_sort dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472629/
https://www.ncbi.nlm.nih.gov/pubmed/37653529
http://dx.doi.org/10.1186/s40104-023-00857-w
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