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Genomic molecular epidemiology of carbapenemase-producing Escherichia coli ST410 isolates by complete genome analysis
The circulation of carbapenemase-producing Escherichia coli (CPEC) in our society is a serious concern for vulnerable patients in nosocomial environments. However, the genomic epidemiology of the circulation of CPEC bacteria among companion animals remains largely unknown. In this study, epidemiolog...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472685/ https://www.ncbi.nlm.nih.gov/pubmed/37658425 http://dx.doi.org/10.1186/s13567-023-01205-6 |
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author | Kyung, Su Min Lee, Junho Lee, Eun-Seo Hwang, Cheol-Yong Yoo, Han Sang |
author_facet | Kyung, Su Min Lee, Junho Lee, Eun-Seo Hwang, Cheol-Yong Yoo, Han Sang |
author_sort | Kyung, Su Min |
collection | PubMed |
description | The circulation of carbapenemase-producing Escherichia coli (CPEC) in our society is a serious concern for vulnerable patients in nosocomial environments. However, the genomic epidemiology of the circulation of CPEC bacteria among companion animals remains largely unknown. In this study, epidemiological analysis was conducted using complete genome identification of CPEC ST410 isolates obtained from companion animals. To estimate the genomic distance and relatedness of the isolates, a total of 37 whole-genome datasets of E. coli ST410 strains were downloaded and comparatively analysed. As a result of the analysis, the genomic structure of the chromosomes and plasmids was identified, revealing the genomic positions of multiple resistance and virulence genes. The isolates in this study were grouped into the subclade H24/RxC, with fimH24, and substituted quinolone resistance-determining regions (QRDRs) and multiple beta-lactamases, including extended-spectrum β-lactamase (ESBL) and carbapenemase. In addition, the in silico comparison of the whole-genome datasets revealed unidentified ST410 H24/Rx subgroups, including either high pathogenicity islands (HPIs) or H21 serotypes. Considering the genetic variations and resistance gene dissemination of the isolates carried by companion animals, future approaches for preventive measurement must include the “One Health” perspective for public health in our society. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-023-01205-6. |
format | Online Article Text |
id | pubmed-10472685 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-104726852023-09-02 Genomic molecular epidemiology of carbapenemase-producing Escherichia coli ST410 isolates by complete genome analysis Kyung, Su Min Lee, Junho Lee, Eun-Seo Hwang, Cheol-Yong Yoo, Han Sang Vet Res Research Article The circulation of carbapenemase-producing Escherichia coli (CPEC) in our society is a serious concern for vulnerable patients in nosocomial environments. However, the genomic epidemiology of the circulation of CPEC bacteria among companion animals remains largely unknown. In this study, epidemiological analysis was conducted using complete genome identification of CPEC ST410 isolates obtained from companion animals. To estimate the genomic distance and relatedness of the isolates, a total of 37 whole-genome datasets of E. coli ST410 strains were downloaded and comparatively analysed. As a result of the analysis, the genomic structure of the chromosomes and plasmids was identified, revealing the genomic positions of multiple resistance and virulence genes. The isolates in this study were grouped into the subclade H24/RxC, with fimH24, and substituted quinolone resistance-determining regions (QRDRs) and multiple beta-lactamases, including extended-spectrum β-lactamase (ESBL) and carbapenemase. In addition, the in silico comparison of the whole-genome datasets revealed unidentified ST410 H24/Rx subgroups, including either high pathogenicity islands (HPIs) or H21 serotypes. Considering the genetic variations and resistance gene dissemination of the isolates carried by companion animals, future approaches for preventive measurement must include the “One Health” perspective for public health in our society. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-023-01205-6. BioMed Central 2023-09-01 2023 /pmc/articles/PMC10472685/ /pubmed/37658425 http://dx.doi.org/10.1186/s13567-023-01205-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Kyung, Su Min Lee, Junho Lee, Eun-Seo Hwang, Cheol-Yong Yoo, Han Sang Genomic molecular epidemiology of carbapenemase-producing Escherichia coli ST410 isolates by complete genome analysis |
title | Genomic molecular epidemiology of carbapenemase-producing Escherichia coli ST410 isolates by complete genome analysis |
title_full | Genomic molecular epidemiology of carbapenemase-producing Escherichia coli ST410 isolates by complete genome analysis |
title_fullStr | Genomic molecular epidemiology of carbapenemase-producing Escherichia coli ST410 isolates by complete genome analysis |
title_full_unstemmed | Genomic molecular epidemiology of carbapenemase-producing Escherichia coli ST410 isolates by complete genome analysis |
title_short | Genomic molecular epidemiology of carbapenemase-producing Escherichia coli ST410 isolates by complete genome analysis |
title_sort | genomic molecular epidemiology of carbapenemase-producing escherichia coli st410 isolates by complete genome analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10472685/ https://www.ncbi.nlm.nih.gov/pubmed/37658425 http://dx.doi.org/10.1186/s13567-023-01205-6 |
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