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Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid

Sertoli cells support the development of sperm and the function of various somatic cells in the interstitium between the tubules. Sertoli cells regulate the function of the testicular vasculature and the development and function of the Leydig cells that produce testosterone for fertility and virilit...

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Autores principales: O’Donnell, Liza, Dagley, Laura F., Curley, Michael, Darbey, Annalucia, O’Shaughnessy, Peter J., Diemer, Thorsten, Pilatz, Adrian, Fietz, Daniela, Stanton, Peter G., Smith, Lee B., Rebourcet, Diane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473511/
https://www.ncbi.nlm.nih.gov/pubmed/37656709
http://dx.doi.org/10.1371/journal.pone.0290846
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author O’Donnell, Liza
Dagley, Laura F.
Curley, Michael
Darbey, Annalucia
O’Shaughnessy, Peter J.
Diemer, Thorsten
Pilatz, Adrian
Fietz, Daniela
Stanton, Peter G.
Smith, Lee B.
Rebourcet, Diane
author_facet O’Donnell, Liza
Dagley, Laura F.
Curley, Michael
Darbey, Annalucia
O’Shaughnessy, Peter J.
Diemer, Thorsten
Pilatz, Adrian
Fietz, Daniela
Stanton, Peter G.
Smith, Lee B.
Rebourcet, Diane
author_sort O’Donnell, Liza
collection PubMed
description Sertoli cells support the development of sperm and the function of various somatic cells in the interstitium between the tubules. Sertoli cells regulate the function of the testicular vasculature and the development and function of the Leydig cells that produce testosterone for fertility and virility. However, the Sertoli cell-derived factors that regulate these cells are largely unknown. To define potential mechanisms by which Sertoli cells could support testicular somatic cell function, we aimed to identify Sertoli cell-enriched proteins in the testicular interstitial fluid (TIF) between the tubules. We previously resolved the proteome of TIF in mice and humans and have shown it to be a rich source of seminiferous tubule-derived proteins. In the current study, we designed bioinformatic strategies to interrogate relevant proteomic and genomic datasets to identify Sertoli cell-enriched proteins in mouse and human TIF. We analysed proteins in mouse TIF that were significantly reduced after one week of acute Sertoli cell ablation in vivo and validated which of these are likely to arise primarily from Sertoli cells based on relevant mouse testis RNASeq datasets. We used a different, but complementary, approach to identify Sertoli cell-enriched proteins in human TIF, taking advantage of high-quality human testis genomic, proteomic and immunohistochemical datasets. We identified a total of 47 and 40 Sertoli cell-enriched proteins in mouse and human TIF, respectively, including 15 proteins that are conserved in both species. Proteins with potential roles in angiogenesis, the regulation of Leydig cells or steroidogenesis, and immune cell regulation were identified. The data suggests that some of these proteins are secreted, but that Sertoli cells also deposit specific proteins into TIF via the release of extracellular vesicles. In conclusion, we have identified novel Sertoli cell-enriched proteins in TIF that are candidates for regulating somatic cell-cell communication and testis function.
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spelling pubmed-104735112023-09-02 Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid O’Donnell, Liza Dagley, Laura F. Curley, Michael Darbey, Annalucia O’Shaughnessy, Peter J. Diemer, Thorsten Pilatz, Adrian Fietz, Daniela Stanton, Peter G. Smith, Lee B. Rebourcet, Diane PLoS One Research Article Sertoli cells support the development of sperm and the function of various somatic cells in the interstitium between the tubules. Sertoli cells regulate the function of the testicular vasculature and the development and function of the Leydig cells that produce testosterone for fertility and virility. However, the Sertoli cell-derived factors that regulate these cells are largely unknown. To define potential mechanisms by which Sertoli cells could support testicular somatic cell function, we aimed to identify Sertoli cell-enriched proteins in the testicular interstitial fluid (TIF) between the tubules. We previously resolved the proteome of TIF in mice and humans and have shown it to be a rich source of seminiferous tubule-derived proteins. In the current study, we designed bioinformatic strategies to interrogate relevant proteomic and genomic datasets to identify Sertoli cell-enriched proteins in mouse and human TIF. We analysed proteins in mouse TIF that were significantly reduced after one week of acute Sertoli cell ablation in vivo and validated which of these are likely to arise primarily from Sertoli cells based on relevant mouse testis RNASeq datasets. We used a different, but complementary, approach to identify Sertoli cell-enriched proteins in human TIF, taking advantage of high-quality human testis genomic, proteomic and immunohistochemical datasets. We identified a total of 47 and 40 Sertoli cell-enriched proteins in mouse and human TIF, respectively, including 15 proteins that are conserved in both species. Proteins with potential roles in angiogenesis, the regulation of Leydig cells or steroidogenesis, and immune cell regulation were identified. The data suggests that some of these proteins are secreted, but that Sertoli cells also deposit specific proteins into TIF via the release of extracellular vesicles. In conclusion, we have identified novel Sertoli cell-enriched proteins in TIF that are candidates for regulating somatic cell-cell communication and testis function. Public Library of Science 2023-09-01 /pmc/articles/PMC10473511/ /pubmed/37656709 http://dx.doi.org/10.1371/journal.pone.0290846 Text en © 2023 O’Donnell et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
O’Donnell, Liza
Dagley, Laura F.
Curley, Michael
Darbey, Annalucia
O’Shaughnessy, Peter J.
Diemer, Thorsten
Pilatz, Adrian
Fietz, Daniela
Stanton, Peter G.
Smith, Lee B.
Rebourcet, Diane
Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid
title Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid
title_full Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid
title_fullStr Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid
title_full_unstemmed Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid
title_short Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid
title_sort sertoli cell-enriched proteins in mouse and human testicular interstitial fluid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473511/
https://www.ncbi.nlm.nih.gov/pubmed/37656709
http://dx.doi.org/10.1371/journal.pone.0290846
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