Designing Efficient Enzymes: Eight Predicted Mutations Convert a Hydroxynitrile Lyase into an Efficient Esterase

Hydroxynitrile lyase from rubber tree (HbHNL) shares 45% identical amino acid residues with the homologous esterase from tobacco, SABP2, but the two enzymes catalyze different reactions. The x-ray structures reveal a serine-histidine-aspartate catalytic triad in both enzymes along with several diffe...

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Detalles Bibliográficos
Autores principales: Casadevall, Guillem, Pierce, Colin, Guan, Bo, Iglesias-Fernandez, Javier, Lim, Huey-Yee, Greenberg, Lauren R., Walsh, Meghan E., Shi, Ke, Gordon, Wendy, Aihara, Hideki, Evans, Robert L., Kazlauskas, Romas, Osuna, Sílvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473745/
https://www.ncbi.nlm.nih.gov/pubmed/37662272
http://dx.doi.org/10.1101/2023.08.23.554512

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