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Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling
Here, we present a protocol to analyze phosphatidylcholine (PC) metabolism in mammalian cells using organelle-selective click labeling coupled with flow cytometry (O-ClickFC). We describe steps for the metabolic incorporation of azide-choline into PC. We then detail fluorescent labeling of the azide...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474069/ https://www.ncbi.nlm.nih.gov/pubmed/37635353 http://dx.doi.org/10.1016/j.xpro.2023.102525 |