Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling

Here, we present a protocol to analyze phosphatidylcholine (PC) metabolism in mammalian cells using organelle-selective click labeling coupled with flow cytometry (O-ClickFC). We describe steps for the metabolic incorporation of azide-choline into PC. We then detail fluorescent labeling of the azide...

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Detalles Bibliográficos
Autores principales: Tsuchiya, Masaki, Tachibana, Nobuhiko, Hamachi, Itaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474069/
https://www.ncbi.nlm.nih.gov/pubmed/37635353
http://dx.doi.org/10.1016/j.xpro.2023.102525

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