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Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy

Red blood cells of vertebrates have undergone evolutionary changes over time, leading to the diversification of morphological and mechanical properties of red blood cells (RBCs). Among the vertebrates, camelids have the most different RBC characteristics. As a result of adaptation to the desert envi...

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Autores principales: Pesen, Tuna, Haydaroglu, Mete, Capar, Simal, Parlatan, Ugur, Unlu, Mehmet Burcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474369/
https://www.ncbi.nlm.nih.gov/pubmed/37664525
http://dx.doi.org/10.1016/j.bbrep.2023.101490
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author Pesen, Tuna
Haydaroglu, Mete
Capar, Simal
Parlatan, Ugur
Unlu, Mehmet Burcin
author_facet Pesen, Tuna
Haydaroglu, Mete
Capar, Simal
Parlatan, Ugur
Unlu, Mehmet Burcin
author_sort Pesen, Tuna
collection PubMed
description Red blood cells of vertebrates have undergone evolutionary changes over time, leading to the diversification of morphological and mechanical properties of red blood cells (RBCs). Among the vertebrates, camelids have the most different RBC characteristics. As a result of adaptation to the desert environment, camelid RBCs can expand twice as much of their total volume in the case of rapid hydration yet are almost undeformable under mechanical stress. In this work, the mechanical and chemical differences in the RBC properties of the human and camelid species were examined using optical tweezers and Raman spectroscopy. We measured the deformability of camel and human RBCs at the single-cell level using optical tweezers. We found that the deformability index (DI) of the camel and the human RBCs were 0.024 ± 0.019 and 0.215 ± 0.061, respectively. To investigate the chemical properties of these cells, we measured the Raman spectra of the whole blood samples. The result of our study indicated that some of the Raman peaks observed on the camel’s blood spectrum were absent in the human blood’s spectrum, which further points to the difference in chemical contents of these two species’ RBCs.
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spelling pubmed-104743692023-09-03 Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy Pesen, Tuna Haydaroglu, Mete Capar, Simal Parlatan, Ugur Unlu, Mehmet Burcin Biochem Biophys Rep Research Article Red blood cells of vertebrates have undergone evolutionary changes over time, leading to the diversification of morphological and mechanical properties of red blood cells (RBCs). Among the vertebrates, camelids have the most different RBC characteristics. As a result of adaptation to the desert environment, camelid RBCs can expand twice as much of their total volume in the case of rapid hydration yet are almost undeformable under mechanical stress. In this work, the mechanical and chemical differences in the RBC properties of the human and camelid species were examined using optical tweezers and Raman spectroscopy. We measured the deformability of camel and human RBCs at the single-cell level using optical tweezers. We found that the deformability index (DI) of the camel and the human RBCs were 0.024 ± 0.019 and 0.215 ± 0.061, respectively. To investigate the chemical properties of these cells, we measured the Raman spectra of the whole blood samples. The result of our study indicated that some of the Raman peaks observed on the camel’s blood spectrum were absent in the human blood’s spectrum, which further points to the difference in chemical contents of these two species’ RBCs. Elsevier 2023-08-24 /pmc/articles/PMC10474369/ /pubmed/37664525 http://dx.doi.org/10.1016/j.bbrep.2023.101490 Text en © 2023 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Pesen, Tuna
Haydaroglu, Mete
Capar, Simal
Parlatan, Ugur
Unlu, Mehmet Burcin
Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy
title Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy
title_full Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy
title_fullStr Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy
title_full_unstemmed Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy
title_short Comparison of the human’s and camel’s red blood cell deformability by optical tweezers and Raman spectroscopy
title_sort comparison of the human’s and camel’s red blood cell deformability by optical tweezers and raman spectroscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474369/
https://www.ncbi.nlm.nih.gov/pubmed/37664525
http://dx.doi.org/10.1016/j.bbrep.2023.101490
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