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A fatty acid-rich fraction of an endolichenic fungus Phoma sp. suppresses immune checkpoint markers via AhR/ARNT and ESR1
Lung cancer has the highest mortality rates worldwide. The disease is caused by environmental pollutants, smoking, and many other factors. Recent treatments include immunotherapeutics, which have shown some success; however, the search for new therapeutics is ongoing. Endolichenic fungi produce a wh...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474435/ https://www.ncbi.nlm.nih.gov/pubmed/37662726 http://dx.doi.org/10.1016/j.heliyon.2023.e19185 |
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author | Varlı, Mücahit Ngo, Men Thi Kim, Seong-Min Taş, İsa Zhou, Rui Gamage, Chathurika D.B. Pulat, Sultan Park, So-Yeon Sesal, Nüzhet Cenk Hur, Jae-Seoun Kang, Kyo Bin Kim, Hangun |
author_facet | Varlı, Mücahit Ngo, Men Thi Kim, Seong-Min Taş, İsa Zhou, Rui Gamage, Chathurika D.B. Pulat, Sultan Park, So-Yeon Sesal, Nüzhet Cenk Hur, Jae-Seoun Kang, Kyo Bin Kim, Hangun |
author_sort | Varlı, Mücahit |
collection | PubMed |
description | Lung cancer has the highest mortality rates worldwide. The disease is caused by environmental pollutants, smoking, and many other factors. Recent treatments include immunotherapeutics, which have shown some success; however, the search for new therapeutics is ongoing. Endolichenic fungi produce a whale of a lot of secondary metabolites, the therapeutic effects of which are being evaluated. Here, we used a crude extract and subfractions of the endolichenic fungus, Phoma sp. (EL006848), isolated from the Pseudevernia furfuracea. It was identified the fatty acid components, palmitic acid, stearic acid, and oleic acid, exist in subfractions E1 and E2. In addition, EL006848 and its fatty acids fractions suppressed benzo[a]pyrene (an AhR ligand)- induced expression of PD-L1 to inhibit the activity of multiple immune checkpoints. E2 subfraction, which had a higher fatty acid content than E1, downregulated expression of AhR/ARNT and several human transcription factors related to ESR1. Moreover, E2 showed a strong inhibitory effect on STAT3 expression and mild effect on NF-kB activity. These results suggest that fatty acids extracted from an endolichenic fungus can exert strong immunotherapeutic effects. |
format | Online Article Text |
id | pubmed-10474435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104744352023-09-03 A fatty acid-rich fraction of an endolichenic fungus Phoma sp. suppresses immune checkpoint markers via AhR/ARNT and ESR1 Varlı, Mücahit Ngo, Men Thi Kim, Seong-Min Taş, İsa Zhou, Rui Gamage, Chathurika D.B. Pulat, Sultan Park, So-Yeon Sesal, Nüzhet Cenk Hur, Jae-Seoun Kang, Kyo Bin Kim, Hangun Heliyon Research Article Lung cancer has the highest mortality rates worldwide. The disease is caused by environmental pollutants, smoking, and many other factors. Recent treatments include immunotherapeutics, which have shown some success; however, the search for new therapeutics is ongoing. Endolichenic fungi produce a whale of a lot of secondary metabolites, the therapeutic effects of which are being evaluated. Here, we used a crude extract and subfractions of the endolichenic fungus, Phoma sp. (EL006848), isolated from the Pseudevernia furfuracea. It was identified the fatty acid components, palmitic acid, stearic acid, and oleic acid, exist in subfractions E1 and E2. In addition, EL006848 and its fatty acids fractions suppressed benzo[a]pyrene (an AhR ligand)- induced expression of PD-L1 to inhibit the activity of multiple immune checkpoints. E2 subfraction, which had a higher fatty acid content than E1, downregulated expression of AhR/ARNT and several human transcription factors related to ESR1. Moreover, E2 showed a strong inhibitory effect on STAT3 expression and mild effect on NF-kB activity. These results suggest that fatty acids extracted from an endolichenic fungus can exert strong immunotherapeutic effects. Elsevier 2023-08-16 /pmc/articles/PMC10474435/ /pubmed/37662726 http://dx.doi.org/10.1016/j.heliyon.2023.e19185 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Varlı, Mücahit Ngo, Men Thi Kim, Seong-Min Taş, İsa Zhou, Rui Gamage, Chathurika D.B. Pulat, Sultan Park, So-Yeon Sesal, Nüzhet Cenk Hur, Jae-Seoun Kang, Kyo Bin Kim, Hangun A fatty acid-rich fraction of an endolichenic fungus Phoma sp. suppresses immune checkpoint markers via AhR/ARNT and ESR1 |
title | A fatty acid-rich fraction of an endolichenic fungus Phoma sp. suppresses immune checkpoint markers via AhR/ARNT and ESR1 |
title_full | A fatty acid-rich fraction of an endolichenic fungus Phoma sp. suppresses immune checkpoint markers via AhR/ARNT and ESR1 |
title_fullStr | A fatty acid-rich fraction of an endolichenic fungus Phoma sp. suppresses immune checkpoint markers via AhR/ARNT and ESR1 |
title_full_unstemmed | A fatty acid-rich fraction of an endolichenic fungus Phoma sp. suppresses immune checkpoint markers via AhR/ARNT and ESR1 |
title_short | A fatty acid-rich fraction of an endolichenic fungus Phoma sp. suppresses immune checkpoint markers via AhR/ARNT and ESR1 |
title_sort | fatty acid-rich fraction of an endolichenic fungus phoma sp. suppresses immune checkpoint markers via ahr/arnt and esr1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474435/ https://www.ncbi.nlm.nih.gov/pubmed/37662726 http://dx.doi.org/10.1016/j.heliyon.2023.e19185 |
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