PCR cloning Intermediated Gibson assembly (PIG) for Constructing DNA Repair Templates in CRISPR-Cas9 Based Gene Editing

The CRISPR-Cas9 gene-editing system has revolutionized genome engineering, allowing precise modifications to be made in a wide range of organisms. One significant challenge associated with CRISPR-Cas9 mediated gene editing is the construction of DNA repair templates containing homology arms, a scree...

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Detalles Bibliográficos
Autores principales: Han, Dongsheng, Churcher, Scott, Nordman, Jared T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474481/
https://www.ncbi.nlm.nih.gov/pubmed/37662052
http://dx.doi.org/10.17912/micropub.biology.000916

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474481/
https://www.ncbi.nlm.nih.gov/pubmed/37662052
http://dx.doi.org/10.17912/micropub.biology.000916

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