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Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil

Despite previous reports of SARS-CoV-2 infection in dogs and cats worldwide, the type of swab sample used for its detection through RT-qPCR needs to be better compared and described. Accordingly, as part of a multicenter study in Brazil, the aim of the present study was to assess which rectal or oro...

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Autores principales: Galhardo, Juliana Arena, Barbosa, David Soeiro, Kmetiuk, Louise Bach, de Carvalho, Otávio Valério, Teixeira, Ana Izabel Passarella, Fonseca, Paula Luize Camargos, de Araújo e Santos, Luiza Campos Guerra, Queiroz, Daniel Costa, Miranda, João Victor Oliveira, da Silva Filho, Aluisio Pereira, Castillo, Anisleidy Pérez, Araujo, Ricardo Nascimento, da Silveira, Julia Angelica Gonçalves, Ristow, Luiz Eduardo, Brandespim, Daniel Friguglietti, Pettan-Brewer, Christina, de Sá Guimarães, Ana Marcia, Dutra, Valéria, de Morais, Helio Autran, dos Santos, Andrea Pires, Agopian, Rafael Garabet, de Aguiar, Renato Santana, Biondo, Alexander Welker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475019/
https://www.ncbi.nlm.nih.gov/pubmed/37660200
http://dx.doi.org/10.1038/s41598-023-41285-0
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author Galhardo, Juliana Arena
Barbosa, David Soeiro
Kmetiuk, Louise Bach
de Carvalho, Otávio Valério
Teixeira, Ana Izabel Passarella
Fonseca, Paula Luize Camargos
de Araújo e Santos, Luiza Campos Guerra
Queiroz, Daniel Costa
Miranda, João Victor Oliveira
da Silva Filho, Aluisio Pereira
Castillo, Anisleidy Pérez
Araujo, Ricardo Nascimento
da Silveira, Julia Angelica Gonçalves
Ristow, Luiz Eduardo
Brandespim, Daniel Friguglietti
Pettan-Brewer, Christina
de Sá Guimarães, Ana Marcia
Dutra, Valéria
de Morais, Helio Autran
dos Santos, Andrea Pires
Agopian, Rafael Garabet
de Aguiar, Renato Santana
Biondo, Alexander Welker
author_facet Galhardo, Juliana Arena
Barbosa, David Soeiro
Kmetiuk, Louise Bach
de Carvalho, Otávio Valério
Teixeira, Ana Izabel Passarella
Fonseca, Paula Luize Camargos
de Araújo e Santos, Luiza Campos Guerra
Queiroz, Daniel Costa
Miranda, João Victor Oliveira
da Silva Filho, Aluisio Pereira
Castillo, Anisleidy Pérez
Araujo, Ricardo Nascimento
da Silveira, Julia Angelica Gonçalves
Ristow, Luiz Eduardo
Brandespim, Daniel Friguglietti
Pettan-Brewer, Christina
de Sá Guimarães, Ana Marcia
Dutra, Valéria
de Morais, Helio Autran
dos Santos, Andrea Pires
Agopian, Rafael Garabet
de Aguiar, Renato Santana
Biondo, Alexander Welker
author_sort Galhardo, Juliana Arena
collection PubMed
description Despite previous reports of SARS-CoV-2 infection in dogs and cats worldwide, the type of swab sample used for its detection through RT-qPCR needs to be better compared and described. Accordingly, as part of a multicenter study in Brazil, the aim of the present study was to assess which rectal or oropharyngeal swabs would be more appropriate for detecting SARS-CoV-2 in cats and dogs, through viral load comparison. Pets of owners diagnosed with COVID-19 in the last 7 days were eligible. A total of 148 animals from four of the five Brazilian geographical regions were analyzed, among which 10/48 cats (20.83%) and 11/100 dogs (11.00%) were positive. The results suggested that oropharyngeal swabs should be considered for SARS-CoV-2 detection, particularly in cats, due to the higher cDNA viral load. Also, the genomic results showed similarities between SARS-CoV-2 animal variants and human variants that were circulating at the time of sampling, thus corroborating the existence of zooanthroponotic transmission. In conclusion, the present study highlighted the importance of SARS-CoV-2 monitoring among cats and dogs, as virus modification may indicate the possibility of mutations in animals and spillover back to owners. Thus, positive individuals should always self-isolate from their pets during COVID-19, to prevent trans-species transmission and mutation.
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spelling pubmed-104750192023-09-04 Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil Galhardo, Juliana Arena Barbosa, David Soeiro Kmetiuk, Louise Bach de Carvalho, Otávio Valério Teixeira, Ana Izabel Passarella Fonseca, Paula Luize Camargos de Araújo e Santos, Luiza Campos Guerra Queiroz, Daniel Costa Miranda, João Victor Oliveira da Silva Filho, Aluisio Pereira Castillo, Anisleidy Pérez Araujo, Ricardo Nascimento da Silveira, Julia Angelica Gonçalves Ristow, Luiz Eduardo Brandespim, Daniel Friguglietti Pettan-Brewer, Christina de Sá Guimarães, Ana Marcia Dutra, Valéria de Morais, Helio Autran dos Santos, Andrea Pires Agopian, Rafael Garabet de Aguiar, Renato Santana Biondo, Alexander Welker Sci Rep Article Despite previous reports of SARS-CoV-2 infection in dogs and cats worldwide, the type of swab sample used for its detection through RT-qPCR needs to be better compared and described. Accordingly, as part of a multicenter study in Brazil, the aim of the present study was to assess which rectal or oropharyngeal swabs would be more appropriate for detecting SARS-CoV-2 in cats and dogs, through viral load comparison. Pets of owners diagnosed with COVID-19 in the last 7 days were eligible. A total of 148 animals from four of the five Brazilian geographical regions were analyzed, among which 10/48 cats (20.83%) and 11/100 dogs (11.00%) were positive. The results suggested that oropharyngeal swabs should be considered for SARS-CoV-2 detection, particularly in cats, due to the higher cDNA viral load. Also, the genomic results showed similarities between SARS-CoV-2 animal variants and human variants that were circulating at the time of sampling, thus corroborating the existence of zooanthroponotic transmission. In conclusion, the present study highlighted the importance of SARS-CoV-2 monitoring among cats and dogs, as virus modification may indicate the possibility of mutations in animals and spillover back to owners. Thus, positive individuals should always self-isolate from their pets during COVID-19, to prevent trans-species transmission and mutation. Nature Publishing Group UK 2023-09-02 /pmc/articles/PMC10475019/ /pubmed/37660200 http://dx.doi.org/10.1038/s41598-023-41285-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Galhardo, Juliana Arena
Barbosa, David Soeiro
Kmetiuk, Louise Bach
de Carvalho, Otávio Valério
Teixeira, Ana Izabel Passarella
Fonseca, Paula Luize Camargos
de Araújo e Santos, Luiza Campos Guerra
Queiroz, Daniel Costa
Miranda, João Victor Oliveira
da Silva Filho, Aluisio Pereira
Castillo, Anisleidy Pérez
Araujo, Ricardo Nascimento
da Silveira, Julia Angelica Gonçalves
Ristow, Luiz Eduardo
Brandespim, Daniel Friguglietti
Pettan-Brewer, Christina
de Sá Guimarães, Ana Marcia
Dutra, Valéria
de Morais, Helio Autran
dos Santos, Andrea Pires
Agopian, Rafael Garabet
de Aguiar, Renato Santana
Biondo, Alexander Welker
Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil
title Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil
title_full Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil
title_fullStr Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil
title_full_unstemmed Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil
title_short Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil
title_sort molecular detection and characterization of sars-cov-2 in cats and dogs of positive owners during the first covid-19 wave in brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475019/
https://www.ncbi.nlm.nih.gov/pubmed/37660200
http://dx.doi.org/10.1038/s41598-023-41285-0
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