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Reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle
Circadian rhythms are regulated by molecular clockwork and drive 24-h behaviors such as locomotor activity, which can be rendered non-functional through genetic knockouts of clock genes. Circadian rhythms are robust in constant darkness (DD) but are modulated to become exactly 24 h by the external d...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475134/ https://www.ncbi.nlm.nih.gov/pubmed/37660212 http://dx.doi.org/10.1038/s41598-023-41029-0 |
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author | Richardson, Melissa E. S. Browne, Chérie-Akilah Mazariegos, Citlali I. Huerta |
author_facet | Richardson, Melissa E. S. Browne, Chérie-Akilah Mazariegos, Citlali I. Huerta |
author_sort | Richardson, Melissa E. S. |
collection | PubMed |
description | Circadian rhythms are regulated by molecular clockwork and drive 24-h behaviors such as locomotor activity, which can be rendered non-functional through genetic knockouts of clock genes. Circadian rhythms are robust in constant darkness (DD) but are modulated to become exactly 24 h by the external day-night cycle. Whether ill-timed light and dark exposure can render circadian behaviors non-functional to the extent of genetic knockouts is less clear. In this study, we discovered an environmental approach that led to a reduction or lack in rhythmic 24-h-circadian wheel-running locomotor behavior in mice (referred to as arrhythmicity). We first observed behavioral circadian arrhythmicity when mice were gradually exposed to a previously published disruptive environment called the fragmented day-night cycle (FDN-G), while maintaining activity alignment with the four dispersed fragments of darkness. Remarkably, upon exposure to constant darkness (DD) or constant light (LL), FDN-G mice lost any resemblance to the FDN-G-only phenotype and instead, exhibited sporadic activity bursts. Circadian rhythms are maintained in control mice with sudden FDN exposure (FDN-S) and fully restored in FDN-G mice either spontaneously in DD or after 12 h:12 h light–dark exposure. This is the first study to generate a light–dark environment that induces reversible suppression of circadian locomotor rhythms in mice. |
format | Online Article Text |
id | pubmed-10475134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104751342023-09-04 Reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle Richardson, Melissa E. S. Browne, Chérie-Akilah Mazariegos, Citlali I. Huerta Sci Rep Article Circadian rhythms are regulated by molecular clockwork and drive 24-h behaviors such as locomotor activity, which can be rendered non-functional through genetic knockouts of clock genes. Circadian rhythms are robust in constant darkness (DD) but are modulated to become exactly 24 h by the external day-night cycle. Whether ill-timed light and dark exposure can render circadian behaviors non-functional to the extent of genetic knockouts is less clear. In this study, we discovered an environmental approach that led to a reduction or lack in rhythmic 24-h-circadian wheel-running locomotor behavior in mice (referred to as arrhythmicity). We first observed behavioral circadian arrhythmicity when mice were gradually exposed to a previously published disruptive environment called the fragmented day-night cycle (FDN-G), while maintaining activity alignment with the four dispersed fragments of darkness. Remarkably, upon exposure to constant darkness (DD) or constant light (LL), FDN-G mice lost any resemblance to the FDN-G-only phenotype and instead, exhibited sporadic activity bursts. Circadian rhythms are maintained in control mice with sudden FDN exposure (FDN-S) and fully restored in FDN-G mice either spontaneously in DD or after 12 h:12 h light–dark exposure. This is the first study to generate a light–dark environment that induces reversible suppression of circadian locomotor rhythms in mice. Nature Publishing Group UK 2023-09-02 /pmc/articles/PMC10475134/ /pubmed/37660212 http://dx.doi.org/10.1038/s41598-023-41029-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Richardson, Melissa E. S. Browne, Chérie-Akilah Mazariegos, Citlali I. Huerta Reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle |
title | Reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle |
title_full | Reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle |
title_fullStr | Reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle |
title_full_unstemmed | Reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle |
title_short | Reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle |
title_sort | reversible suppression of circadian-driven locomotor rhythms in mice using a gradual fragmentation of the day-night cycle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475134/ https://www.ncbi.nlm.nih.gov/pubmed/37660212 http://dx.doi.org/10.1038/s41598-023-41029-0 |
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