Cargando…

Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle

Heat acclimatization or acclimation training in horses is practiced to reduce physiological strain and improve exercise performance in the heat, which can involve metabolic improvement in skeletal muscle. However, there is limited information concerning the acute signaling responses of equine skelet...

Descripción completa

Detalles Bibliográficos
Autores principales: Ebisuda, Yusaku, Mukai, Kazutaka, Takahashi, Yuji, Yoshida, Toshinobu, Kawano, Aoto, Matsuhashi, Tsubasa, Miyata, Hirofumi, Kuwahara, Masayoshi, Ohmura, Hajime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475567/
https://www.ncbi.nlm.nih.gov/pubmed/37671280
http://dx.doi.org/10.3389/fvets.2023.1230212
_version_ 1785100746250780672
author Ebisuda, Yusaku
Mukai, Kazutaka
Takahashi, Yuji
Yoshida, Toshinobu
Kawano, Aoto
Matsuhashi, Tsubasa
Miyata, Hirofumi
Kuwahara, Masayoshi
Ohmura, Hajime
author_facet Ebisuda, Yusaku
Mukai, Kazutaka
Takahashi, Yuji
Yoshida, Toshinobu
Kawano, Aoto
Matsuhashi, Tsubasa
Miyata, Hirofumi
Kuwahara, Masayoshi
Ohmura, Hajime
author_sort Ebisuda, Yusaku
collection PubMed
description Heat acclimatization or acclimation training in horses is practiced to reduce physiological strain and improve exercise performance in the heat, which can involve metabolic improvement in skeletal muscle. However, there is limited information concerning the acute signaling responses of equine skeletal muscle after exercise in a hot environment. The purpose of this study was to investigate the hypothesis that exercise in hot conditions induces greater changes in heat shock proteins and mitochondrial-related signaling in equine skeletal muscle compared with exercise in cool conditions. Fifteen trained Thoroughbred horses [4.6 ± 0.4 (mean ± SE) years old; 503 ± 14 kg] were assigned to perform a treadmill exercise test in cool conditions [COOL; Wet Bulb Globe Temperature (WBGT), 12.5°C; n = 8] or hot conditions (HOT; WBGT, 29.5°C; n = 7) consisting of walking at 1.7 m/s for 1 min, trotting at 4 m/s for 5 min, and cantering at 7 m/s for 2 min and at 90% of VO(2max) for 2 min, followed by walking at 1.7 m/s for 20 min. Heart rate during exercise and plasma lactate concentration immediately after exercise were measured. Biopsy samples were obtained from the middle gluteal muscle before and at 4 h after exercise, and relative quantitative analysis of mRNA expression using real-time RT-PCR was performed. Data were analyzed with using mixed models. There were no significant differences between the two groups in peak heart rate (COOL, 213 ± 3 bpm; HOT, 214 ± 4 bpm; p = 0.782) and plasma lactate concentration (COOL, 13.1 ± 1.4 mmoL/L; HOT, 17.5 ± 1.7 mmoL/L; p = 0.060), while HSP-70 (COOL, 1.9-fold, p = 0.207; HOT, 2.4-fold, p = 0.045), PGC-1α (COOL, 3.8-fold, p = 0.424; HOT, 8.4-fold, p = 0.010), HIF-1α (COOL, 1.6-fold, p = 0.315; HOT, 2.2-fold, p = 0.018) and PDK4 (COOL, 7.6-fold, p = 0.412; HOT, 14.1-fold, p = 0.047) mRNA increased significantly only in HOT at 4 h after exercise. These data indicate that acute exercise in a hot environment facilitates protective response to heat stress (HSP-70), mitochondrial biogenesis (PGC-1α and HIF-1α) and fatty acid oxidation (PDK4).
format Online
Article
Text
id pubmed-10475567
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-104755672023-09-05 Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle Ebisuda, Yusaku Mukai, Kazutaka Takahashi, Yuji Yoshida, Toshinobu Kawano, Aoto Matsuhashi, Tsubasa Miyata, Hirofumi Kuwahara, Masayoshi Ohmura, Hajime Front Vet Sci Veterinary Science Heat acclimatization or acclimation training in horses is practiced to reduce physiological strain and improve exercise performance in the heat, which can involve metabolic improvement in skeletal muscle. However, there is limited information concerning the acute signaling responses of equine skeletal muscle after exercise in a hot environment. The purpose of this study was to investigate the hypothesis that exercise in hot conditions induces greater changes in heat shock proteins and mitochondrial-related signaling in equine skeletal muscle compared with exercise in cool conditions. Fifteen trained Thoroughbred horses [4.6 ± 0.4 (mean ± SE) years old; 503 ± 14 kg] were assigned to perform a treadmill exercise test in cool conditions [COOL; Wet Bulb Globe Temperature (WBGT), 12.5°C; n = 8] or hot conditions (HOT; WBGT, 29.5°C; n = 7) consisting of walking at 1.7 m/s for 1 min, trotting at 4 m/s for 5 min, and cantering at 7 m/s for 2 min and at 90% of VO(2max) for 2 min, followed by walking at 1.7 m/s for 20 min. Heart rate during exercise and plasma lactate concentration immediately after exercise were measured. Biopsy samples were obtained from the middle gluteal muscle before and at 4 h after exercise, and relative quantitative analysis of mRNA expression using real-time RT-PCR was performed. Data were analyzed with using mixed models. There were no significant differences between the two groups in peak heart rate (COOL, 213 ± 3 bpm; HOT, 214 ± 4 bpm; p = 0.782) and plasma lactate concentration (COOL, 13.1 ± 1.4 mmoL/L; HOT, 17.5 ± 1.7 mmoL/L; p = 0.060), while HSP-70 (COOL, 1.9-fold, p = 0.207; HOT, 2.4-fold, p = 0.045), PGC-1α (COOL, 3.8-fold, p = 0.424; HOT, 8.4-fold, p = 0.010), HIF-1α (COOL, 1.6-fold, p = 0.315; HOT, 2.2-fold, p = 0.018) and PDK4 (COOL, 7.6-fold, p = 0.412; HOT, 14.1-fold, p = 0.047) mRNA increased significantly only in HOT at 4 h after exercise. These data indicate that acute exercise in a hot environment facilitates protective response to heat stress (HSP-70), mitochondrial biogenesis (PGC-1α and HIF-1α) and fatty acid oxidation (PDK4). Frontiers Media S.A. 2023-08-21 /pmc/articles/PMC10475567/ /pubmed/37671280 http://dx.doi.org/10.3389/fvets.2023.1230212 Text en Copyright © 2023 Ebisuda, Mukai, Takahashi, Yoshida, Kawano, Matsuhashi, Miyata, Kuwahara and Ohmura. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Ebisuda, Yusaku
Mukai, Kazutaka
Takahashi, Yuji
Yoshida, Toshinobu
Kawano, Aoto
Matsuhashi, Tsubasa
Miyata, Hirofumi
Kuwahara, Masayoshi
Ohmura, Hajime
Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle
title Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle
title_full Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle
title_fullStr Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle
title_full_unstemmed Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle
title_short Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle
title_sort acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mrna in thoroughbred horse skeletal muscle
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475567/
https://www.ncbi.nlm.nih.gov/pubmed/37671280
http://dx.doi.org/10.3389/fvets.2023.1230212
work_keys_str_mv AT ebisudayusaku acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle
AT mukaikazutaka acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle
AT takahashiyuji acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle
AT yoshidatoshinobu acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle
AT kawanoaoto acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle
AT matsuhashitsubasa acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle
AT miyatahirofumi acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle
AT kuwaharamasayoshi acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle
AT ohmurahajime acuteexerciseinahotenvironmentincreasesheatshockprotein70andperoxisomeproliferatoractivatedreceptorgcoactivator1amrnainthoroughbredhorseskeletalmuscle