A massively parallel approach for assessing CRISPR off-targets in vitro

In a recent issue of Med, Tian et al.(1) present AID-seq, an approach that enables massively parallel identification of off-targets for different CRISPR nucleases in vitro. By using a pooled strategy to simultaneously identify the on-/off-targets of multiple gRNAs, the authors could screen the most...

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Detalles Bibliográficos
Autores principales: Huang, Shisheng, Huang, Xingxu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Spotlight
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475838/
https://www.ncbi.nlm.nih.gov/pubmed/37671012
http://dx.doi.org/10.1016/j.crmeth.2023.100561
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author Huang, Shisheng
Huang, Xingxu
author_facet Huang, Shisheng
Huang, Xingxu
author_sort Huang, Shisheng
collection PubMed
description In a recent issue of Med, Tian et al.(1) present AID-seq, an approach that enables massively parallel identification of off-targets for different CRISPR nucleases in vitro. By using a pooled strategy to simultaneously identify the on-/off-targets of multiple gRNAs, the authors could screen the most efficient and safe gRNA candidates.
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spelling pubmed-104758382023-09-05 A massively parallel approach for assessing CRISPR off-targets in vitro Huang, Shisheng Huang, Xingxu Cell Rep Methods Spotlight In a recent issue of Med, Tian et al.(1) present AID-seq, an approach that enables massively parallel identification of off-targets for different CRISPR nucleases in vitro. By using a pooled strategy to simultaneously identify the on-/off-targets of multiple gRNAs, the authors could screen the most efficient and safe gRNA candidates. Elsevier 2023-08-28 /pmc/articles/PMC10475838/ /pubmed/37671012 http://dx.doi.org/10.1016/j.crmeth.2023.100561 Text en © 2023. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Spotlight
Huang, Shisheng
Huang, Xingxu
A massively parallel approach for assessing CRISPR off-targets in vitro
title A massively parallel approach for assessing CRISPR off-targets in vitro
title_full A massively parallel approach for assessing CRISPR off-targets in vitro
title_fullStr A massively parallel approach for assessing CRISPR off-targets in vitro
title_full_unstemmed A massively parallel approach for assessing CRISPR off-targets in vitro
title_short A massively parallel approach for assessing CRISPR off-targets in vitro
title_sort massively parallel approach for assessing crispr off-targets in vitro
topic Spotlight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475838/
https://www.ncbi.nlm.nih.gov/pubmed/37671012
http://dx.doi.org/10.1016/j.crmeth.2023.100561
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