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A massively parallel approach for assessing CRISPR off-targets in vitro
In a recent issue of Med, Tian et al.(1) present AID-seq, an approach that enables massively parallel identification of off-targets for different CRISPR nucleases in vitro. By using a pooled strategy to simultaneously identify the on-/off-targets of multiple gRNAs, the authors could screen the most...
Autores principales: | Huang, Shisheng, Huang, Xingxu |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475838/ https://www.ncbi.nlm.nih.gov/pubmed/37671012 http://dx.doi.org/10.1016/j.crmeth.2023.100561 |
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