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In vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived Bacillus velezensis AG6 exopolysaccharide

In the current study, Bacillus velezensis AG6 was isolated from sediment samples in the Red Sea, identified by traditional microbiological techniques and phylogenetic 16S rRNA sequences. Among eight isolates screened for exopolysaccharide (EPS) production, the R6 isolate was the highest producer wit...

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Autores principales: Alharbi, Maha A., Alrehaili, Amani A., Albureikan, Mona Othman I., Gharib, Amal F., Daghistani, Hussam, Bakhuraysah, Maha M., Aloraini, Ghfren S., Bazuhair, Mohammed A., Alhuthali, Hayaa M., Ghareeb, Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10476021/
https://www.ncbi.nlm.nih.gov/pubmed/37671337
http://dx.doi.org/10.1039/d3ra04009g
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author Alharbi, Maha A.
Alrehaili, Amani A.
Albureikan, Mona Othman I.
Gharib, Amal F.
Daghistani, Hussam
Bakhuraysah, Maha M.
Aloraini, Ghfren S.
Bazuhair, Mohammed A.
Alhuthali, Hayaa M.
Ghareeb, Ahmed
author_facet Alharbi, Maha A.
Alrehaili, Amani A.
Albureikan, Mona Othman I.
Gharib, Amal F.
Daghistani, Hussam
Bakhuraysah, Maha M.
Aloraini, Ghfren S.
Bazuhair, Mohammed A.
Alhuthali, Hayaa M.
Ghareeb, Ahmed
author_sort Alharbi, Maha A.
collection PubMed
description In the current study, Bacillus velezensis AG6 was isolated from sediment samples in the Red Sea, identified by traditional microbiological techniques and phylogenetic 16S rRNA sequences. Among eight isolates screened for exopolysaccharide (EPS) production, the R6 isolate was the highest producer with a significant fraction of EPS (EPSF6, 5.79 g L(−1)). The EPSF6 molecule was found to have a molecular weight (Mw) of 2.7 × 10(4) g mol(−1) and a number average (Mn) of 2.6 × 10(4) g mol(−1) when it was analyzed using GPC. The FTIR spectrum indicated no sulfate but uronic acid (43.8%). According to HPLC, the EPSF6 fraction’s monosaccharides were xylose, galactose, and galacturonic acid in a molar ratio of 2.0 : 0.5 : 2.0. DPPH, H(2)O(2), and ABTS tests assessed EPSF6’s antioxidant capabilities at 100, 300, 500, 1000, and 1500 μg mL(−1) for 15, 60, 45, and 60 minutes. The overall antioxidant activities were dose- and time-dependently increased, and improved by increasing concentrations from 100 to 1500 μg mL(−1) after 60 minutes and found to be 91.34 ± 1.1%, 80.20 ± 1.4% and 75.28 ± 1.1% respectively. Next, EPSF6 displayed considerable inhibitory activity toward the proliferation of six cancerous cell lines. Anti-inflammatory tests were performed using lipoxygenase (5-LOX) and cyclooxygenase (COX-2). An MTP turbidity assay method was applied to show the ability of EPSF6 to inhibit Gram-positive bacteria, Gram-negative bacteria, and antibiofilm formation. Together, this study sheds light on the potential pharmacological applications of a secondary metabolite produced by marine Bacillus velezensis AG6. Its expected impact on human health will increase as more research and studies are conducted globally.
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spelling pubmed-104760212023-09-05 In vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived Bacillus velezensis AG6 exopolysaccharide Alharbi, Maha A. Alrehaili, Amani A. Albureikan, Mona Othman I. Gharib, Amal F. Daghistani, Hussam Bakhuraysah, Maha M. Aloraini, Ghfren S. Bazuhair, Mohammed A. Alhuthali, Hayaa M. Ghareeb, Ahmed RSC Adv Chemistry In the current study, Bacillus velezensis AG6 was isolated from sediment samples in the Red Sea, identified by traditional microbiological techniques and phylogenetic 16S rRNA sequences. Among eight isolates screened for exopolysaccharide (EPS) production, the R6 isolate was the highest producer with a significant fraction of EPS (EPSF6, 5.79 g L(−1)). The EPSF6 molecule was found to have a molecular weight (Mw) of 2.7 × 10(4) g mol(−1) and a number average (Mn) of 2.6 × 10(4) g mol(−1) when it was analyzed using GPC. The FTIR spectrum indicated no sulfate but uronic acid (43.8%). According to HPLC, the EPSF6 fraction’s monosaccharides were xylose, galactose, and galacturonic acid in a molar ratio of 2.0 : 0.5 : 2.0. DPPH, H(2)O(2), and ABTS tests assessed EPSF6’s antioxidant capabilities at 100, 300, 500, 1000, and 1500 μg mL(−1) for 15, 60, 45, and 60 minutes. The overall antioxidant activities were dose- and time-dependently increased, and improved by increasing concentrations from 100 to 1500 μg mL(−1) after 60 minutes and found to be 91.34 ± 1.1%, 80.20 ± 1.4% and 75.28 ± 1.1% respectively. Next, EPSF6 displayed considerable inhibitory activity toward the proliferation of six cancerous cell lines. Anti-inflammatory tests were performed using lipoxygenase (5-LOX) and cyclooxygenase (COX-2). An MTP turbidity assay method was applied to show the ability of EPSF6 to inhibit Gram-positive bacteria, Gram-negative bacteria, and antibiofilm formation. Together, this study sheds light on the potential pharmacological applications of a secondary metabolite produced by marine Bacillus velezensis AG6. Its expected impact on human health will increase as more research and studies are conducted globally. The Royal Society of Chemistry 2023-09-04 /pmc/articles/PMC10476021/ /pubmed/37671337 http://dx.doi.org/10.1039/d3ra04009g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Alharbi, Maha A.
Alrehaili, Amani A.
Albureikan, Mona Othman I.
Gharib, Amal F.
Daghistani, Hussam
Bakhuraysah, Maha M.
Aloraini, Ghfren S.
Bazuhair, Mohammed A.
Alhuthali, Hayaa M.
Ghareeb, Ahmed
In vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived Bacillus velezensis AG6 exopolysaccharide
title In vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived Bacillus velezensis AG6 exopolysaccharide
title_full In vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived Bacillus velezensis AG6 exopolysaccharide
title_fullStr In vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived Bacillus velezensis AG6 exopolysaccharide
title_full_unstemmed In vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived Bacillus velezensis AG6 exopolysaccharide
title_short In vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived Bacillus velezensis AG6 exopolysaccharide
title_sort in vitro studies on the pharmacological potential, anti-tumor, antimicrobial, and acetylcholinesterase inhibitory activity of marine-derived bacillus velezensis ag6 exopolysaccharide
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10476021/
https://www.ncbi.nlm.nih.gov/pubmed/37671337
http://dx.doi.org/10.1039/d3ra04009g
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