Cargando…

Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis

Vibriosis is caused by some pathogenic Vibrio and produces significant mortality in Pacific white shrimp Penaeus (Litopenaeus) vannamei larvae in commercial hatcheries. Acute hepatopancreatic necrosis disease (AHPND) is an emerging vibriosis affecting shrimp-producing countries worldwide. Zoea 2 syn...

Descripción completa

Detalles Bibliográficos
Autores principales: Reyes, Guillermo, Andrade, Betsy, Betancourt, Irma, Panchana, Fanny, Solórzano, Ramiro, Preciado, Cristhian, Sorroza, Lita, Trujillo, Luis E., Bayot, Bonny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10476614/
https://www.ncbi.nlm.nih.gov/pubmed/37671363
http://dx.doi.org/10.7717/peerj.15795
_version_ 1785100971559354368
author Reyes, Guillermo
Andrade, Betsy
Betancourt, Irma
Panchana, Fanny
Solórzano, Ramiro
Preciado, Cristhian
Sorroza, Lita
Trujillo, Luis E.
Bayot, Bonny
author_facet Reyes, Guillermo
Andrade, Betsy
Betancourt, Irma
Panchana, Fanny
Solórzano, Ramiro
Preciado, Cristhian
Sorroza, Lita
Trujillo, Luis E.
Bayot, Bonny
author_sort Reyes, Guillermo
collection PubMed
description Vibriosis is caused by some pathogenic Vibrio and produces significant mortality in Pacific white shrimp Penaeus (Litopenaeus) vannamei larvae in commercial hatcheries. Acute hepatopancreatic necrosis disease (AHPND) is an emerging vibriosis affecting shrimp-producing countries worldwide. Zoea 2 syndrome is another type of vibriosis that affects the early stages of P. vannamei larvae. Although the pathogenesis of AHPND and zoea 2 syndrome is well known, there is scarce information about microbial composition and biomarkers of P.vannamei larvae affected by AHPND, and there is no study of the microbiome of larvae affected by zoea 2 syndrome. In this work, we characterized the microbiome of P. vannamei larvae collected from 12 commercial hatchery tanks by high-throughput sequencing. Seven tanks were affected by AHPND, and five tanks were affected by zoea 2 syndrome. Subsequently, all samples were selected for sequencing of the V3–V4 region of the16S rRNA gene. Similarity analysis using the beta diversity index revealed significant differences in the larval bacterial communities between disease conditions, particularly when Vibrio was analyzed. Linear discriminant analysis with effect size determined specific microbial signatures for AHPND and zoea 2 syndrome. Sneathiella, Cyclobacterium, Haliea, Lewinella, among other genera, were abundant in AHPND-affected larvae. Meanwhile, Vibrio, Spongiimonas, Meridianimaribacter, Tenacibaculum, among other genera, were significantly abundant in larvae affected by zoea 2 syndrome. The bacterial network at the phylum level for larvae collected from tanks affected by AHPND showed greater complexity and connectivity than in samples collected from tanks affected by zoea 2 syndrome. The bacterial connections inter Vibrio genera were higher in larvae from tanks affected by zoea 2 syndrome, also presenting other connections between the genera Vibrio and Catenococcus. The identification of specific biomarkers found in this study could be useful for understanding the microbial dynamics during different types of vibriosis.
format Online
Article
Text
id pubmed-10476614
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-104766142023-09-05 Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis Reyes, Guillermo Andrade, Betsy Betancourt, Irma Panchana, Fanny Solórzano, Ramiro Preciado, Cristhian Sorroza, Lita Trujillo, Luis E. Bayot, Bonny PeerJ Aquaculture, Fisheries and Fish Science Vibriosis is caused by some pathogenic Vibrio and produces significant mortality in Pacific white shrimp Penaeus (Litopenaeus) vannamei larvae in commercial hatcheries. Acute hepatopancreatic necrosis disease (AHPND) is an emerging vibriosis affecting shrimp-producing countries worldwide. Zoea 2 syndrome is another type of vibriosis that affects the early stages of P. vannamei larvae. Although the pathogenesis of AHPND and zoea 2 syndrome is well known, there is scarce information about microbial composition and biomarkers of P.vannamei larvae affected by AHPND, and there is no study of the microbiome of larvae affected by zoea 2 syndrome. In this work, we characterized the microbiome of P. vannamei larvae collected from 12 commercial hatchery tanks by high-throughput sequencing. Seven tanks were affected by AHPND, and five tanks were affected by zoea 2 syndrome. Subsequently, all samples were selected for sequencing of the V3–V4 region of the16S rRNA gene. Similarity analysis using the beta diversity index revealed significant differences in the larval bacterial communities between disease conditions, particularly when Vibrio was analyzed. Linear discriminant analysis with effect size determined specific microbial signatures for AHPND and zoea 2 syndrome. Sneathiella, Cyclobacterium, Haliea, Lewinella, among other genera, were abundant in AHPND-affected larvae. Meanwhile, Vibrio, Spongiimonas, Meridianimaribacter, Tenacibaculum, among other genera, were significantly abundant in larvae affected by zoea 2 syndrome. The bacterial network at the phylum level for larvae collected from tanks affected by AHPND showed greater complexity and connectivity than in samples collected from tanks affected by zoea 2 syndrome. The bacterial connections inter Vibrio genera were higher in larvae from tanks affected by zoea 2 syndrome, also presenting other connections between the genera Vibrio and Catenococcus. The identification of specific biomarkers found in this study could be useful for understanding the microbial dynamics during different types of vibriosis. PeerJ Inc. 2023-09-01 /pmc/articles/PMC10476614/ /pubmed/37671363 http://dx.doi.org/10.7717/peerj.15795 Text en ©2023 Reyes et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Reyes, Guillermo
Andrade, Betsy
Betancourt, Irma
Panchana, Fanny
Solórzano, Ramiro
Preciado, Cristhian
Sorroza, Lita
Trujillo, Luis E.
Bayot, Bonny
Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis
title Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis
title_full Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis
title_fullStr Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis
title_full_unstemmed Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis
title_short Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis
title_sort microbial signature profiles of penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10476614/
https://www.ncbi.nlm.nih.gov/pubmed/37671363
http://dx.doi.org/10.7717/peerj.15795
work_keys_str_mv AT reyesguillermo microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis
AT andradebetsy microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis
AT betancourtirma microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis
AT panchanafanny microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis
AT solorzanoramiro microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis
AT preciadocristhian microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis
AT sorrozalita microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis
AT trujilloluise microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis
AT bayotbonny microbialsignatureprofilesofpenaeusvannameilarvaeinlowsurvivalhatcherytanksaffectedbyvibriosis