Metal-enhanced fluorescence through conventional Ag-polyethylene glycol nanoparticles for cellular imaging

A novel application of conventional Ag nanoparticles (NPs) for metal-enhanced fluorescence (MEF) in cellular imaging is proposed. Different molecular weights of polyethylene glycol (PEG) were tested to determine a suitable spacer on Ag NPs for MEF, and NPs comprising Ag with PEG with a molecular wei...

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Detalles Bibliográficos
Autores principales: Chen, Chih-Jung, Wu, Chun-Yen, Wu, Chi-Wei, Chang, Ching-Wen, Huang, Tsung-Tao, Shiao, Ming-Hua, Lin, Chu-Kuei, Chen, Yu-Chun, Lin, Yung-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477826/
https://www.ncbi.nlm.nih.gov/pubmed/37674489
http://dx.doi.org/10.1039/d3ra02277c
Descripción
Sumario:A novel application of conventional Ag nanoparticles (NPs) for metal-enhanced fluorescence (MEF) in cellular imaging is proposed. Different molecular weights of polyethylene glycol (PEG) were tested to determine a suitable spacer on Ag NPs for MEF, and NPs comprising Ag with PEG with a molecular weight of 6000 g (Ag-PEG6k), when present in fluorescein solution, were discovered to cause a 2-fold quantum yield enhancement. For fluorescence imaging of mesenchymal stem cells stained by Alexa Fluor 488, the enhancement factor increased with the Ag-PEG6k NP concentration but decreased with the Alexa Fluor 488 concentration. At 243 parts per billion Ag-PEG6k NPs and 625 parts per million Alexa Fluor 488, the enhancement factor reached its greatest value of over 4.

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