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The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma

Accumulation of vimentin is the core event in epithelial–mesenchymal transition (EMT). Post‐translational modifications have been widely reported to play crucial roles in imparting different properties and functions to vimentin. Here, a novel modification of vimentin, acetylated at Lys(104) (vimenti...

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Autores principales: Yang, Rui, Peng, Weilin, Shi, Shuai, Peng, Xiong, Cai, Qidong, Zhao, Zhenyu, He, Boxue, Tu, Guangxu, Yin, Wei, Chen, Yichuan, Zhang, Yuqian, Liu, Fang, Wang, Xiang, Xiao, Desheng, Tao, Yongguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477884/
https://www.ncbi.nlm.nih.gov/pubmed/37424170
http://dx.doi.org/10.1002/advs.202300971
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author Yang, Rui
Peng, Weilin
Shi, Shuai
Peng, Xiong
Cai, Qidong
Zhao, Zhenyu
He, Boxue
Tu, Guangxu
Yin, Wei
Chen, Yichuan
Zhang, Yuqian
Liu, Fang
Wang, Xiang
Xiao, Desheng
Tao, Yongguang
author_facet Yang, Rui
Peng, Weilin
Shi, Shuai
Peng, Xiong
Cai, Qidong
Zhao, Zhenyu
He, Boxue
Tu, Guangxu
Yin, Wei
Chen, Yichuan
Zhang, Yuqian
Liu, Fang
Wang, Xiang
Xiao, Desheng
Tao, Yongguang
author_sort Yang, Rui
collection PubMed
description Accumulation of vimentin is the core event in epithelial–mesenchymal transition (EMT). Post‐translational modifications have been widely reported to play crucial roles in imparting different properties and functions to vimentin. Here, a novel modification of vimentin, acetylated at Lys(104) (vimentin‐K104Ac) is identified, which is stable in lung adenocarcinoma (LUAD) cells. Mechanistically, NACHT, LRR, and PYD domain‐containing protein 11 (NLRP11), a regulator of the inflammatory response, bind to vimentin and promote vimentin‐K104Ac expression, which is highly expressed in the early stages of LUAD and frequently appears in vimentin‐positive LUAD tissues. In addition, it is observed that an acetyltransferase, lysine acetyltransferase 7 (KAT7), which binds to NLRP11 and vimentin, directly mediates the acetylation of vimentin at Lys(104) and that the cytoplasmic localization of KAT7 can be induced by NLRP11. Malignant promotion mediated by transfection with vimentin‐K104Q is noticeably greater than that mediated by transfection with vimentin‐WT. Further, suppressing the effects of NLRP11 and KAT7 on vimentin noticeably inhibited the malignant behavior of vimentin‐positive LUAD in vivo and in vitro. In summary, these findings have established a relationship between inflammation and EMT, which is reflected via KAT7‐mediated acetylation of vimentin at Lys(104) dependent on NLRP11.
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spelling pubmed-104778842023-09-06 The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma Yang, Rui Peng, Weilin Shi, Shuai Peng, Xiong Cai, Qidong Zhao, Zhenyu He, Boxue Tu, Guangxu Yin, Wei Chen, Yichuan Zhang, Yuqian Liu, Fang Wang, Xiang Xiao, Desheng Tao, Yongguang Adv Sci (Weinh) Research Articles Accumulation of vimentin is the core event in epithelial–mesenchymal transition (EMT). Post‐translational modifications have been widely reported to play crucial roles in imparting different properties and functions to vimentin. Here, a novel modification of vimentin, acetylated at Lys(104) (vimentin‐K104Ac) is identified, which is stable in lung adenocarcinoma (LUAD) cells. Mechanistically, NACHT, LRR, and PYD domain‐containing protein 11 (NLRP11), a regulator of the inflammatory response, bind to vimentin and promote vimentin‐K104Ac expression, which is highly expressed in the early stages of LUAD and frequently appears in vimentin‐positive LUAD tissues. In addition, it is observed that an acetyltransferase, lysine acetyltransferase 7 (KAT7), which binds to NLRP11 and vimentin, directly mediates the acetylation of vimentin at Lys(104) and that the cytoplasmic localization of KAT7 can be induced by NLRP11. Malignant promotion mediated by transfection with vimentin‐K104Q is noticeably greater than that mediated by transfection with vimentin‐WT. Further, suppressing the effects of NLRP11 and KAT7 on vimentin noticeably inhibited the malignant behavior of vimentin‐positive LUAD in vivo and in vitro. In summary, these findings have established a relationship between inflammation and EMT, which is reflected via KAT7‐mediated acetylation of vimentin at Lys(104) dependent on NLRP11. John Wiley and Sons Inc. 2023-07-09 /pmc/articles/PMC10477884/ /pubmed/37424170 http://dx.doi.org/10.1002/advs.202300971 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Yang, Rui
Peng, Weilin
Shi, Shuai
Peng, Xiong
Cai, Qidong
Zhao, Zhenyu
He, Boxue
Tu, Guangxu
Yin, Wei
Chen, Yichuan
Zhang, Yuqian
Liu, Fang
Wang, Xiang
Xiao, Desheng
Tao, Yongguang
The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma
title The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma
title_full The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma
title_fullStr The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma
title_full_unstemmed The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma
title_short The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma
title_sort nlrp11 protein bridges the histone lysine acetyltransferase kat7 to acetylate vimentin in the early stage of lung adenocarcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477884/
https://www.ncbi.nlm.nih.gov/pubmed/37424170
http://dx.doi.org/10.1002/advs.202300971
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