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The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma
Accumulation of vimentin is the core event in epithelial–mesenchymal transition (EMT). Post‐translational modifications have been widely reported to play crucial roles in imparting different properties and functions to vimentin. Here, a novel modification of vimentin, acetylated at Lys(104) (vimenti...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477884/ https://www.ncbi.nlm.nih.gov/pubmed/37424170 http://dx.doi.org/10.1002/advs.202300971 |
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author | Yang, Rui Peng, Weilin Shi, Shuai Peng, Xiong Cai, Qidong Zhao, Zhenyu He, Boxue Tu, Guangxu Yin, Wei Chen, Yichuan Zhang, Yuqian Liu, Fang Wang, Xiang Xiao, Desheng Tao, Yongguang |
author_facet | Yang, Rui Peng, Weilin Shi, Shuai Peng, Xiong Cai, Qidong Zhao, Zhenyu He, Boxue Tu, Guangxu Yin, Wei Chen, Yichuan Zhang, Yuqian Liu, Fang Wang, Xiang Xiao, Desheng Tao, Yongguang |
author_sort | Yang, Rui |
collection | PubMed |
description | Accumulation of vimentin is the core event in epithelial–mesenchymal transition (EMT). Post‐translational modifications have been widely reported to play crucial roles in imparting different properties and functions to vimentin. Here, a novel modification of vimentin, acetylated at Lys(104) (vimentin‐K104Ac) is identified, which is stable in lung adenocarcinoma (LUAD) cells. Mechanistically, NACHT, LRR, and PYD domain‐containing protein 11 (NLRP11), a regulator of the inflammatory response, bind to vimentin and promote vimentin‐K104Ac expression, which is highly expressed in the early stages of LUAD and frequently appears in vimentin‐positive LUAD tissues. In addition, it is observed that an acetyltransferase, lysine acetyltransferase 7 (KAT7), which binds to NLRP11 and vimentin, directly mediates the acetylation of vimentin at Lys(104) and that the cytoplasmic localization of KAT7 can be induced by NLRP11. Malignant promotion mediated by transfection with vimentin‐K104Q is noticeably greater than that mediated by transfection with vimentin‐WT. Further, suppressing the effects of NLRP11 and KAT7 on vimentin noticeably inhibited the malignant behavior of vimentin‐positive LUAD in vivo and in vitro. In summary, these findings have established a relationship between inflammation and EMT, which is reflected via KAT7‐mediated acetylation of vimentin at Lys(104) dependent on NLRP11. |
format | Online Article Text |
id | pubmed-10477884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104778842023-09-06 The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma Yang, Rui Peng, Weilin Shi, Shuai Peng, Xiong Cai, Qidong Zhao, Zhenyu He, Boxue Tu, Guangxu Yin, Wei Chen, Yichuan Zhang, Yuqian Liu, Fang Wang, Xiang Xiao, Desheng Tao, Yongguang Adv Sci (Weinh) Research Articles Accumulation of vimentin is the core event in epithelial–mesenchymal transition (EMT). Post‐translational modifications have been widely reported to play crucial roles in imparting different properties and functions to vimentin. Here, a novel modification of vimentin, acetylated at Lys(104) (vimentin‐K104Ac) is identified, which is stable in lung adenocarcinoma (LUAD) cells. Mechanistically, NACHT, LRR, and PYD domain‐containing protein 11 (NLRP11), a regulator of the inflammatory response, bind to vimentin and promote vimentin‐K104Ac expression, which is highly expressed in the early stages of LUAD and frequently appears in vimentin‐positive LUAD tissues. In addition, it is observed that an acetyltransferase, lysine acetyltransferase 7 (KAT7), which binds to NLRP11 and vimentin, directly mediates the acetylation of vimentin at Lys(104) and that the cytoplasmic localization of KAT7 can be induced by NLRP11. Malignant promotion mediated by transfection with vimentin‐K104Q is noticeably greater than that mediated by transfection with vimentin‐WT. Further, suppressing the effects of NLRP11 and KAT7 on vimentin noticeably inhibited the malignant behavior of vimentin‐positive LUAD in vivo and in vitro. In summary, these findings have established a relationship between inflammation and EMT, which is reflected via KAT7‐mediated acetylation of vimentin at Lys(104) dependent on NLRP11. John Wiley and Sons Inc. 2023-07-09 /pmc/articles/PMC10477884/ /pubmed/37424170 http://dx.doi.org/10.1002/advs.202300971 Text en © 2023 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Yang, Rui Peng, Weilin Shi, Shuai Peng, Xiong Cai, Qidong Zhao, Zhenyu He, Boxue Tu, Guangxu Yin, Wei Chen, Yichuan Zhang, Yuqian Liu, Fang Wang, Xiang Xiao, Desheng Tao, Yongguang The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma |
title | The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma |
title_full | The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma |
title_fullStr | The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma |
title_full_unstemmed | The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma |
title_short | The NLRP11 Protein Bridges the Histone Lysine Acetyltransferase KAT7 to Acetylate Vimentin in the Early Stage of Lung Adenocarcinoma |
title_sort | nlrp11 protein bridges the histone lysine acetyltransferase kat7 to acetylate vimentin in the early stage of lung adenocarcinoma |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477884/ https://www.ncbi.nlm.nih.gov/pubmed/37424170 http://dx.doi.org/10.1002/advs.202300971 |
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