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Development and optimization of an in-house heterologous ELISA for detection of prednisolone drug in enzyme conjugates using spacers
The introduction of spacers in coating steroid protein complexes and/or enzyme conjugates or immunogens is known to exert an influence on the sensitivity of steroid enzyme immunoassays. We investigated the impact of different homobifunctional spacers, ranging in atomic length from 3 to 10, on the se...
Autores principales: | Kumar, Dinesh, Oberoi, Harinder Singh, Singh, Harpal, Shrivastav, Tulsidas G., Bhukya, Prudhvi Lal, Kumari, Mansi, Koner, Bidhan Chandra, Sonkar, Subash Chandra |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477981/ https://www.ncbi.nlm.nih.gov/pubmed/37675116 http://dx.doi.org/10.3389/fimmu.2023.1200328 |
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