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Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance

BACKGROUND: Probiotics are used in the broiler industry to increase production performance. Most often a probiotic is applied by mixing it in the feed, but studies have shown that earlier application may be advantageous. Therefore, in ovo application where the probiotic is administrated into the egg...

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Autores principales: Villumsen, Kasper Rømer, Sandvang, Dorthe, Vestergård, Gisle, Olsen, Mia Son Räfle, Juul, Johanne, Dencker, Morten, Kudsk, Johannes, Poulsen, Louise Ladefoged
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10478294/
https://www.ncbi.nlm.nih.gov/pubmed/37670379
http://dx.doi.org/10.1186/s42523-023-00263-7
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author Villumsen, Kasper Rømer
Sandvang, Dorthe
Vestergård, Gisle
Olsen, Mia Son Räfle
Juul, Johanne
Dencker, Morten
Kudsk, Johannes
Poulsen, Louise Ladefoged
author_facet Villumsen, Kasper Rømer
Sandvang, Dorthe
Vestergård, Gisle
Olsen, Mia Son Räfle
Juul, Johanne
Dencker, Morten
Kudsk, Johannes
Poulsen, Louise Ladefoged
author_sort Villumsen, Kasper Rømer
collection PubMed
description BACKGROUND: Probiotics are used in the broiler industry to increase production performance. Most often a probiotic is applied by mixing it in the feed, but studies have shown that earlier application may be advantageous. Therefore, in ovo application where the probiotic is administrated into the egg before hatch has been investigated as an alternative application method. However, in ovo application may impact hatchability negatively and may not be feasible at all hatcheries. The purpose of this study was to investigate the effect of a novel non-invasive method for mass application before hatch. The probiotic (E. faecium 669) was applied as a single dose by spray on the unhatched eggs and production performance and development of the cecal microbiota until slaughter was compared with a control flock. Through 16S rRNA sequencing of cecal samples from 25 broilers at day 7, 21 and 37 we compared the microbiota composition and richness for each group. The study was repeated for additional recording of production performance and re-isolation of the probiotic E. faecium from the intestine. RESULTS: In both trials the probiotic E. faecium could be re-isolated from the yolk sac and intestine at hatch and at day 7. Broilers in the probiotic treated groups had a higher performance in terms of bodyweight at day 34 and European production efficiency factor. Finally, a significant reduction of first-week and overall mortality was observed in the probiotic group in the first trial. Based on 16S rRNA profiling, significant differences in alpha diversity were found exclusively at day 37. Estimation of beta diversities, however, identified significant differences in microbiota composition between the control and probiotic group at day 7, 21 and 37. CONCLUSION: The probiotic E. faecium strain successfully colonized broilers before/during hatch after a single spray application at day 18 of incubation. Positive effects of the probiotic were observed in multiple production parameters, including reduced mortality in trial 1, and microbiota analyses indicate significantly different microbiota compositions throughout the experimental phase. Taken together, the novel low-tech mass administration of E. faecium (669) may be considered a feasible strategy for improvements of production parameters in broiler production. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-023-00263-7.
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spelling pubmed-104782942023-09-06 Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance Villumsen, Kasper Rømer Sandvang, Dorthe Vestergård, Gisle Olsen, Mia Son Räfle Juul, Johanne Dencker, Morten Kudsk, Johannes Poulsen, Louise Ladefoged Anim Microbiome Research BACKGROUND: Probiotics are used in the broiler industry to increase production performance. Most often a probiotic is applied by mixing it in the feed, but studies have shown that earlier application may be advantageous. Therefore, in ovo application where the probiotic is administrated into the egg before hatch has been investigated as an alternative application method. However, in ovo application may impact hatchability negatively and may not be feasible at all hatcheries. The purpose of this study was to investigate the effect of a novel non-invasive method for mass application before hatch. The probiotic (E. faecium 669) was applied as a single dose by spray on the unhatched eggs and production performance and development of the cecal microbiota until slaughter was compared with a control flock. Through 16S rRNA sequencing of cecal samples from 25 broilers at day 7, 21 and 37 we compared the microbiota composition and richness for each group. The study was repeated for additional recording of production performance and re-isolation of the probiotic E. faecium from the intestine. RESULTS: In both trials the probiotic E. faecium could be re-isolated from the yolk sac and intestine at hatch and at day 7. Broilers in the probiotic treated groups had a higher performance in terms of bodyweight at day 34 and European production efficiency factor. Finally, a significant reduction of first-week and overall mortality was observed in the probiotic group in the first trial. Based on 16S rRNA profiling, significant differences in alpha diversity were found exclusively at day 37. Estimation of beta diversities, however, identified significant differences in microbiota composition between the control and probiotic group at day 7, 21 and 37. CONCLUSION: The probiotic E. faecium strain successfully colonized broilers before/during hatch after a single spray application at day 18 of incubation. Positive effects of the probiotic were observed in multiple production parameters, including reduced mortality in trial 1, and microbiota analyses indicate significantly different microbiota compositions throughout the experimental phase. Taken together, the novel low-tech mass administration of E. faecium (669) may be considered a feasible strategy for improvements of production parameters in broiler production. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42523-023-00263-7. BioMed Central 2023-09-05 /pmc/articles/PMC10478294/ /pubmed/37670379 http://dx.doi.org/10.1186/s42523-023-00263-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Villumsen, Kasper Rømer
Sandvang, Dorthe
Vestergård, Gisle
Olsen, Mia Son Räfle
Juul, Johanne
Dencker, Morten
Kudsk, Johannes
Poulsen, Louise Ladefoged
Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance
title Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance
title_full Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance
title_fullStr Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance
title_full_unstemmed Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance
title_short Effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance
title_sort effects of a novel, non-invasive pre-hatch application of probiotic for broilers on development of cecum microbiota and production performance
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10478294/
https://www.ncbi.nlm.nih.gov/pubmed/37670379
http://dx.doi.org/10.1186/s42523-023-00263-7
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