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The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model

BACKGROUND: This research aimed to evaluate the protective effects of Artemisia Absinthium L. (Abs) against liver damage induced by aluminium oxide nanoparticles (Al(2)O(3) NPs) in rats, including both structural and functional changes associated with hepatotoxicity. METHODS: Thirty-six rats were ra...

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Autores principales: Karami, Esmaeil, Goodarzi, Zahra, Shahtaheri, Seyed Jamaleddin, Kiani, Mehrafarin, Faridan, Mohammad, Ghazi-Khansari, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10478434/
https://www.ncbi.nlm.nih.gov/pubmed/37670294
http://dx.doi.org/10.1186/s12906-023-04121-6
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author Karami, Esmaeil
Goodarzi, Zahra
Shahtaheri, Seyed Jamaleddin
Kiani, Mehrafarin
Faridan, Mohammad
Ghazi-Khansari, Mahmoud
author_facet Karami, Esmaeil
Goodarzi, Zahra
Shahtaheri, Seyed Jamaleddin
Kiani, Mehrafarin
Faridan, Mohammad
Ghazi-Khansari, Mahmoud
author_sort Karami, Esmaeil
collection PubMed
description BACKGROUND: This research aimed to evaluate the protective effects of Artemisia Absinthium L. (Abs) against liver damage induced by aluminium oxide nanoparticles (Al(2)O(3) NPs) in rats, including both structural and functional changes associated with hepatotoxicity. METHODS: Thirty-six rats were randomly divided into six groups (n = 6). The first group received no treatment. The second group was orally administered Abs at a dose of 200 mg/kg/b.w. The third and fifth groups were injected intraperitoneally with γ-Al(2)O(3) NPs and α-Al(2)O(3) NPs, respectively, at a dose of 30 mg/kg/b.w. The fourth and sixth groups were pre-treated with oral Abs at a dose of 200 mg/kg/b.w. along with intraperitoneal injection of γ-Al(2)O(3) NPs and α-Al(2)O(3) NPs, respectively, at a dose of 30 mg/kg/b.w. RESULTS: Treatment with γ-Al(2)O(3) NPs resulted in a significant decrease (P < 0.05) in total body weight gain, relative liver weight to body weight, and liver weight in rats. However, co-administration of γ-Al(2)O(3) NPs with Abs significantly increased body weight gain (P < 0.05). Rats treated with Al(2)O(3) NPs (γ and α) exhibited elevated levels of malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), alanine transaminase (ALT), and aspartate aminotransferase (AST). Conversely, treatment significantly reduced glutathione peroxidase (GPx), catalase (CAT), total superoxide dismutase (T-SOD), and total antioxidant capacity (TAC) levels compared to the control group. Furthermore, the expression of heme oxygenase-1 (HO-1) and metallothionein-1 (MT-1) mRNAs, cytochrome P450 (CYP P450) protein, and histopathological changes were significantly up-regulated in rats injected with Al(2)O(3) NPs. Pre-treatment with Abs significantly reduced MDA, AST, HO-1, and CYP P450 levels in the liver, while increasing GPx and T-SOD levels compared to rats treated with Al(2)O(3) NPs. CONCLUSION: The results indicate that Abs has potential protective effects against oxidative stress, up-regulation of oxidative-related genes and proteins, and histopathological alterations induced by Al(2)O(3) NPs. Notably, γ-Al(2)O(3) NPs exhibited greater hepatotoxicity than α-Al(2)O(3) NPs.
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spelling pubmed-104784342023-09-06 The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model Karami, Esmaeil Goodarzi, Zahra Shahtaheri, Seyed Jamaleddin Kiani, Mehrafarin Faridan, Mohammad Ghazi-Khansari, Mahmoud BMC Complement Med Ther Research BACKGROUND: This research aimed to evaluate the protective effects of Artemisia Absinthium L. (Abs) against liver damage induced by aluminium oxide nanoparticles (Al(2)O(3) NPs) in rats, including both structural and functional changes associated with hepatotoxicity. METHODS: Thirty-six rats were randomly divided into six groups (n = 6). The first group received no treatment. The second group was orally administered Abs at a dose of 200 mg/kg/b.w. The third and fifth groups were injected intraperitoneally with γ-Al(2)O(3) NPs and α-Al(2)O(3) NPs, respectively, at a dose of 30 mg/kg/b.w. The fourth and sixth groups were pre-treated with oral Abs at a dose of 200 mg/kg/b.w. along with intraperitoneal injection of γ-Al(2)O(3) NPs and α-Al(2)O(3) NPs, respectively, at a dose of 30 mg/kg/b.w. RESULTS: Treatment with γ-Al(2)O(3) NPs resulted in a significant decrease (P < 0.05) in total body weight gain, relative liver weight to body weight, and liver weight in rats. However, co-administration of γ-Al(2)O(3) NPs with Abs significantly increased body weight gain (P < 0.05). Rats treated with Al(2)O(3) NPs (γ and α) exhibited elevated levels of malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), alanine transaminase (ALT), and aspartate aminotransferase (AST). Conversely, treatment significantly reduced glutathione peroxidase (GPx), catalase (CAT), total superoxide dismutase (T-SOD), and total antioxidant capacity (TAC) levels compared to the control group. Furthermore, the expression of heme oxygenase-1 (HO-1) and metallothionein-1 (MT-1) mRNAs, cytochrome P450 (CYP P450) protein, and histopathological changes were significantly up-regulated in rats injected with Al(2)O(3) NPs. Pre-treatment with Abs significantly reduced MDA, AST, HO-1, and CYP P450 levels in the liver, while increasing GPx and T-SOD levels compared to rats treated with Al(2)O(3) NPs. CONCLUSION: The results indicate that Abs has potential protective effects against oxidative stress, up-regulation of oxidative-related genes and proteins, and histopathological alterations induced by Al(2)O(3) NPs. Notably, γ-Al(2)O(3) NPs exhibited greater hepatotoxicity than α-Al(2)O(3) NPs. BioMed Central 2023-09-05 /pmc/articles/PMC10478434/ /pubmed/37670294 http://dx.doi.org/10.1186/s12906-023-04121-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Karami, Esmaeil
Goodarzi, Zahra
Shahtaheri, Seyed Jamaleddin
Kiani, Mehrafarin
Faridan, Mohammad
Ghazi-Khansari, Mahmoud
The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model
title The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model
title_full The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model
title_fullStr The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model
title_full_unstemmed The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model
title_short The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model
title_sort aqueous extract of artemisia absinthium l. stimulates ho-1/mt-1/cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10478434/
https://www.ncbi.nlm.nih.gov/pubmed/37670294
http://dx.doi.org/10.1186/s12906-023-04121-6
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