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Top-down mass spectrometry of native proteoforms and their complexes: A community study

The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spect...

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Detalles Bibliográficos
Autores principales: Lermyte, Frederik, Habeck, Tanja, Brown, Kyle, Des Soye, Benjamin, Lantz, Carter, Zhou, Mowei, Alam, Novera, Hossain, Md Amin, Jung, Wonhyeuk, Keener, James, Volny, Michael, Wilson, Jesse, Ying, Yujia, Agar, Jeffrey, Danis, Paul, Ge, Ying, Kelleher, Neil, Li, Huilin, Loo, Joseph, Marty, Michael, Pasa-Tolic, Ljiljana, Sandoval, Wendy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479449/
https://www.ncbi.nlm.nih.gov/pubmed/37674709
http://dx.doi.org/10.21203/rs.3.rs-3228472/v1
Descripción
Sumario:The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spectrometry and top-down mass spectrometry are not yet well standardised, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics (CTDP) initiated a study to develop and test protocols for native mass spectrometry combined with top-down fragmentation of proteins and protein complexes across eleven instruments in nine laboratories. The outcomes are summarised in this report to provide robust benchmarks and a valuable entry point for the scientific community.