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Top-down mass spectrometry of native proteoforms and their complexes: A community study

The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spect...

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Autores principales: Lermyte, Frederik, Habeck, Tanja, Brown, Kyle, Des Soye, Benjamin, Lantz, Carter, Zhou, Mowei, Alam, Novera, Hossain, Md Amin, Jung, Wonhyeuk, Keener, James, Volny, Michael, Wilson, Jesse, Ying, Yujia, Agar, Jeffrey, Danis, Paul, Ge, Ying, Kelleher, Neil, Li, Huilin, Loo, Joseph, Marty, Michael, Pasa-Tolic, Ljiljana, Sandoval, Wendy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479449/
https://www.ncbi.nlm.nih.gov/pubmed/37674709
http://dx.doi.org/10.21203/rs.3.rs-3228472/v1
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author Lermyte, Frederik
Habeck, Tanja
Brown, Kyle
Des Soye, Benjamin
Lantz, Carter
Zhou, Mowei
Alam, Novera
Hossain, Md Amin
Jung, Wonhyeuk
Keener, James
Volny, Michael
Wilson, Jesse
Ying, Yujia
Agar, Jeffrey
Danis, Paul
Ge, Ying
Kelleher, Neil
Li, Huilin
Loo, Joseph
Marty, Michael
Pasa-Tolic, Ljiljana
Sandoval, Wendy
author_facet Lermyte, Frederik
Habeck, Tanja
Brown, Kyle
Des Soye, Benjamin
Lantz, Carter
Zhou, Mowei
Alam, Novera
Hossain, Md Amin
Jung, Wonhyeuk
Keener, James
Volny, Michael
Wilson, Jesse
Ying, Yujia
Agar, Jeffrey
Danis, Paul
Ge, Ying
Kelleher, Neil
Li, Huilin
Loo, Joseph
Marty, Michael
Pasa-Tolic, Ljiljana
Sandoval, Wendy
author_sort Lermyte, Frederik
collection PubMed
description The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spectrometry and top-down mass spectrometry are not yet well standardised, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics (CTDP) initiated a study to develop and test protocols for native mass spectrometry combined with top-down fragmentation of proteins and protein complexes across eleven instruments in nine laboratories. The outcomes are summarised in this report to provide robust benchmarks and a valuable entry point for the scientific community.
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spelling pubmed-104794492023-09-06 Top-down mass spectrometry of native proteoforms and their complexes: A community study Lermyte, Frederik Habeck, Tanja Brown, Kyle Des Soye, Benjamin Lantz, Carter Zhou, Mowei Alam, Novera Hossain, Md Amin Jung, Wonhyeuk Keener, James Volny, Michael Wilson, Jesse Ying, Yujia Agar, Jeffrey Danis, Paul Ge, Ying Kelleher, Neil Li, Huilin Loo, Joseph Marty, Michael Pasa-Tolic, Ljiljana Sandoval, Wendy Res Sq Article The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spectrometry and top-down mass spectrometry are not yet well standardised, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics (CTDP) initiated a study to develop and test protocols for native mass spectrometry combined with top-down fragmentation of proteins and protein complexes across eleven instruments in nine laboratories. The outcomes are summarised in this report to provide robust benchmarks and a valuable entry point for the scientific community. American Journal Experts 2023-08-23 /pmc/articles/PMC10479449/ /pubmed/37674709 http://dx.doi.org/10.21203/rs.3.rs-3228472/v1 Text en https://creativecommons.org/licenses/by/4.0/License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License (https://creativecommons.org/licenses/by/4.0/) https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Lermyte, Frederik
Habeck, Tanja
Brown, Kyle
Des Soye, Benjamin
Lantz, Carter
Zhou, Mowei
Alam, Novera
Hossain, Md Amin
Jung, Wonhyeuk
Keener, James
Volny, Michael
Wilson, Jesse
Ying, Yujia
Agar, Jeffrey
Danis, Paul
Ge, Ying
Kelleher, Neil
Li, Huilin
Loo, Joseph
Marty, Michael
Pasa-Tolic, Ljiljana
Sandoval, Wendy
Top-down mass spectrometry of native proteoforms and their complexes: A community study
title Top-down mass spectrometry of native proteoforms and their complexes: A community study
title_full Top-down mass spectrometry of native proteoforms and their complexes: A community study
title_fullStr Top-down mass spectrometry of native proteoforms and their complexes: A community study
title_full_unstemmed Top-down mass spectrometry of native proteoforms and their complexes: A community study
title_short Top-down mass spectrometry of native proteoforms and their complexes: A community study
title_sort top-down mass spectrometry of native proteoforms and their complexes: a community study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479449/
https://www.ncbi.nlm.nih.gov/pubmed/37674709
http://dx.doi.org/10.21203/rs.3.rs-3228472/v1
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