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Top-down mass spectrometry of native proteoforms and their complexes: A community study
The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spect...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Journal Experts
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479449/ https://www.ncbi.nlm.nih.gov/pubmed/37674709 http://dx.doi.org/10.21203/rs.3.rs-3228472/v1 |
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author | Lermyte, Frederik Habeck, Tanja Brown, Kyle Des Soye, Benjamin Lantz, Carter Zhou, Mowei Alam, Novera Hossain, Md Amin Jung, Wonhyeuk Keener, James Volny, Michael Wilson, Jesse Ying, Yujia Agar, Jeffrey Danis, Paul Ge, Ying Kelleher, Neil Li, Huilin Loo, Joseph Marty, Michael Pasa-Tolic, Ljiljana Sandoval, Wendy |
author_facet | Lermyte, Frederik Habeck, Tanja Brown, Kyle Des Soye, Benjamin Lantz, Carter Zhou, Mowei Alam, Novera Hossain, Md Amin Jung, Wonhyeuk Keener, James Volny, Michael Wilson, Jesse Ying, Yujia Agar, Jeffrey Danis, Paul Ge, Ying Kelleher, Neil Li, Huilin Loo, Joseph Marty, Michael Pasa-Tolic, Ljiljana Sandoval, Wendy |
author_sort | Lermyte, Frederik |
collection | PubMed |
description | The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spectrometry and top-down mass spectrometry are not yet well standardised, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics (CTDP) initiated a study to develop and test protocols for native mass spectrometry combined with top-down fragmentation of proteins and protein complexes across eleven instruments in nine laboratories. The outcomes are summarised in this report to provide robust benchmarks and a valuable entry point for the scientific community. |
format | Online Article Text |
id | pubmed-10479449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Journal Experts |
record_format | MEDLINE/PubMed |
spelling | pubmed-104794492023-09-06 Top-down mass spectrometry of native proteoforms and their complexes: A community study Lermyte, Frederik Habeck, Tanja Brown, Kyle Des Soye, Benjamin Lantz, Carter Zhou, Mowei Alam, Novera Hossain, Md Amin Jung, Wonhyeuk Keener, James Volny, Michael Wilson, Jesse Ying, Yujia Agar, Jeffrey Danis, Paul Ge, Ying Kelleher, Neil Li, Huilin Loo, Joseph Marty, Michael Pasa-Tolic, Ljiljana Sandoval, Wendy Res Sq Article The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spectrometry and top-down mass spectrometry are not yet well standardised, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics (CTDP) initiated a study to develop and test protocols for native mass spectrometry combined with top-down fragmentation of proteins and protein complexes across eleven instruments in nine laboratories. The outcomes are summarised in this report to provide robust benchmarks and a valuable entry point for the scientific community. American Journal Experts 2023-08-23 /pmc/articles/PMC10479449/ /pubmed/37674709 http://dx.doi.org/10.21203/rs.3.rs-3228472/v1 Text en https://creativecommons.org/licenses/by/4.0/License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License (https://creativecommons.org/licenses/by/4.0/) https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Lermyte, Frederik Habeck, Tanja Brown, Kyle Des Soye, Benjamin Lantz, Carter Zhou, Mowei Alam, Novera Hossain, Md Amin Jung, Wonhyeuk Keener, James Volny, Michael Wilson, Jesse Ying, Yujia Agar, Jeffrey Danis, Paul Ge, Ying Kelleher, Neil Li, Huilin Loo, Joseph Marty, Michael Pasa-Tolic, Ljiljana Sandoval, Wendy Top-down mass spectrometry of native proteoforms and their complexes: A community study |
title | Top-down mass spectrometry of native proteoforms and their complexes: A community study |
title_full | Top-down mass spectrometry of native proteoforms and their complexes: A community study |
title_fullStr | Top-down mass spectrometry of native proteoforms and their complexes: A community study |
title_full_unstemmed | Top-down mass spectrometry of native proteoforms and their complexes: A community study |
title_short | Top-down mass spectrometry of native proteoforms and their complexes: A community study |
title_sort | top-down mass spectrometry of native proteoforms and their complexes: a community study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479449/ https://www.ncbi.nlm.nih.gov/pubmed/37674709 http://dx.doi.org/10.21203/rs.3.rs-3228472/v1 |
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