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Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice

Recent studies have found that erythropoietin promotes the recovery of neurological function after traumatic brain injury. However, the precise mechanism of action remains unclear. In this study, we induced moderate traumatic brain injury in mice by intraperitoneal injection of erythropoietin for 3...

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Autores principales: Tan, Weilin, Ma, Jun, Fu, Jiayuanyuan, Wu, Biying, Zhu, Ziyu, Huang, Xuekang, Du, Mengran, Wu, Chenrui, Balawi, Ehab, Zhou, Qiang, Zhang, Jie, Liao, Zhengbu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479836/
https://www.ncbi.nlm.nih.gov/pubmed/37488864
http://dx.doi.org/10.4103/1673-5374.374135
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author Tan, Weilin
Ma, Jun
Fu, Jiayuanyuan
Wu, Biying
Zhu, Ziyu
Huang, Xuekang
Du, Mengran
Wu, Chenrui
Balawi, Ehab
Zhou, Qiang
Zhang, Jie
Liao, Zhengbu
author_facet Tan, Weilin
Ma, Jun
Fu, Jiayuanyuan
Wu, Biying
Zhu, Ziyu
Huang, Xuekang
Du, Mengran
Wu, Chenrui
Balawi, Ehab
Zhou, Qiang
Zhang, Jie
Liao, Zhengbu
author_sort Tan, Weilin
collection PubMed
description Recent studies have found that erythropoietin promotes the recovery of neurological function after traumatic brain injury. However, the precise mechanism of action remains unclear. In this study, we induced moderate traumatic brain injury in mice by intraperitoneal injection of erythropoietin for 3 consecutive days. RNA sequencing detected a total of 4065 differentially expressed RNAs, including 1059 mRNAs, 92 microRNAs, 799 long non-coding RNAs, and 2115 circular RNAs. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses revealed that the coding and non-coding RNAs that were differentially expressed after traumatic brain injury and treatment with erythropoietin play roles in the axon guidance pathway, Wnt pathway, and MAPK pathway. Constructing competing endogenous RNA networks showed that regulatory relationship between the differentially expressed non-coding RNAs and mRNAs. Because the axon guidance pathway was repeatedly enriched, the expression of Wnt5a and Ephb6, key factors in the axonal guidance pathway, was assessed. Ephb6 expression decreased and Wnt5a expression increased after traumatic brain injury, and these effects were reversed by treatment with erythropoietin. These findings suggest that erythropoietin can promote recovery of nerve function after traumatic brain injury through the axon guidance pathway.
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spelling pubmed-104798362023-09-06 Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice Tan, Weilin Ma, Jun Fu, Jiayuanyuan Wu, Biying Zhu, Ziyu Huang, Xuekang Du, Mengran Wu, Chenrui Balawi, Ehab Zhou, Qiang Zhang, Jie Liao, Zhengbu Neural Regen Res Research Article Recent studies have found that erythropoietin promotes the recovery of neurological function after traumatic brain injury. However, the precise mechanism of action remains unclear. In this study, we induced moderate traumatic brain injury in mice by intraperitoneal injection of erythropoietin for 3 consecutive days. RNA sequencing detected a total of 4065 differentially expressed RNAs, including 1059 mRNAs, 92 microRNAs, 799 long non-coding RNAs, and 2115 circular RNAs. Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses revealed that the coding and non-coding RNAs that were differentially expressed after traumatic brain injury and treatment with erythropoietin play roles in the axon guidance pathway, Wnt pathway, and MAPK pathway. Constructing competing endogenous RNA networks showed that regulatory relationship between the differentially expressed non-coding RNAs and mRNAs. Because the axon guidance pathway was repeatedly enriched, the expression of Wnt5a and Ephb6, key factors in the axonal guidance pathway, was assessed. Ephb6 expression decreased and Wnt5a expression increased after traumatic brain injury, and these effects were reversed by treatment with erythropoietin. These findings suggest that erythropoietin can promote recovery of nerve function after traumatic brain injury through the axon guidance pathway. Wolters Kluwer - Medknow 2023-04-20 /pmc/articles/PMC10479836/ /pubmed/37488864 http://dx.doi.org/10.4103/1673-5374.374135 Text en Copyright: © Neural Regeneration Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Research Article
Tan, Weilin
Ma, Jun
Fu, Jiayuanyuan
Wu, Biying
Zhu, Ziyu
Huang, Xuekang
Du, Mengran
Wu, Chenrui
Balawi, Ehab
Zhou, Qiang
Zhang, Jie
Liao, Zhengbu
Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice
title Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice
title_full Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice
title_fullStr Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice
title_full_unstemmed Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice
title_short Transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice
title_sort transcriptomic and bioinformatics analysis of the mechanism by which erythropoietin promotes recovery from traumatic brain injury in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479836/
https://www.ncbi.nlm.nih.gov/pubmed/37488864
http://dx.doi.org/10.4103/1673-5374.374135
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