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Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice
Glaucoma is a leading cause of irreversible blindness worldwide, and previous studies have shown that, in addition to affecting the eyes, it also causes abnormalities in the brain. However, it is not yet clear how the primary visual cortex (V1) is altered in glaucoma. This study used DBA/2J mice as...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479843/ https://www.ncbi.nlm.nih.gov/pubmed/37488870 http://dx.doi.org/10.4103/1673-5374.375341 |
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author | Yang, Yin Yang, Zhaoxi Lv, Maoxia Jia, Ang Li, Junjun Liao, Baitao Chen, Jing’an Wu, Zhengzheng Shi, Yi Xia, Yang Yao, Dezhong Chen, Ke |
author_facet | Yang, Yin Yang, Zhaoxi Lv, Maoxia Jia, Ang Li, Junjun Liao, Baitao Chen, Jing’an Wu, Zhengzheng Shi, Yi Xia, Yang Yao, Dezhong Chen, Ke |
author_sort | Yang, Yin |
collection | PubMed |
description | Glaucoma is a leading cause of irreversible blindness worldwide, and previous studies have shown that, in addition to affecting the eyes, it also causes abnormalities in the brain. However, it is not yet clear how the primary visual cortex (V1) is altered in glaucoma. This study used DBA/2J mice as a model for spontaneous secondary glaucoma. The aim of the study was to compare the electrophysiological and histomorphological characteristics of neurons in the V1 between 9-month-old DBA/2J mice and age-matched C57BL/6J mice. We conducted single-unit recordings in the V1 of light-anesthetized mice to measure the visually induced responses, including single-unit spiking and gamma band oscillations. The morphology of layer II/III neurons was determined by neuronal nuclear antigen staining and Nissl staining of brain tissue sections. Eighty-seven neurons from eight DBA/2J mice and eighty-one neurons from eight C57BL/6J mice were examined. Compared with the C57BL/6J group, V1 neurons in the DBA/2J group exhibited weaker visual tuning and impaired spatial summation. Moreover, fewer neurons were observed in the V1 of DBA/2J mice compared with C57BL/6J mice. These findings suggest that DBA/2J mice have fewer neurons in the V1 compared with C57BL/6J mice, and that these neurons have impaired visual tuning. Our findings provide a better understanding of the pathological changes that occur in V1 neuron function and morphology in the DBA/2J mouse model. This study might offer some innovative perspectives regarding the treatment of glaucoma. |
format | Online Article Text |
id | pubmed-10479843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-104798432023-09-06 Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice Yang, Yin Yang, Zhaoxi Lv, Maoxia Jia, Ang Li, Junjun Liao, Baitao Chen, Jing’an Wu, Zhengzheng Shi, Yi Xia, Yang Yao, Dezhong Chen, Ke Neural Regen Res Research Article Glaucoma is a leading cause of irreversible blindness worldwide, and previous studies have shown that, in addition to affecting the eyes, it also causes abnormalities in the brain. However, it is not yet clear how the primary visual cortex (V1) is altered in glaucoma. This study used DBA/2J mice as a model for spontaneous secondary glaucoma. The aim of the study was to compare the electrophysiological and histomorphological characteristics of neurons in the V1 between 9-month-old DBA/2J mice and age-matched C57BL/6J mice. We conducted single-unit recordings in the V1 of light-anesthetized mice to measure the visually induced responses, including single-unit spiking and gamma band oscillations. The morphology of layer II/III neurons was determined by neuronal nuclear antigen staining and Nissl staining of brain tissue sections. Eighty-seven neurons from eight DBA/2J mice and eighty-one neurons from eight C57BL/6J mice were examined. Compared with the C57BL/6J group, V1 neurons in the DBA/2J group exhibited weaker visual tuning and impaired spatial summation. Moreover, fewer neurons were observed in the V1 of DBA/2J mice compared with C57BL/6J mice. These findings suggest that DBA/2J mice have fewer neurons in the V1 compared with C57BL/6J mice, and that these neurons have impaired visual tuning. Our findings provide a better understanding of the pathological changes that occur in V1 neuron function and morphology in the DBA/2J mouse model. This study might offer some innovative perspectives regarding the treatment of glaucoma. Wolters Kluwer - Medknow 2023-05-31 /pmc/articles/PMC10479843/ /pubmed/37488870 http://dx.doi.org/10.4103/1673-5374.375341 Text en Copyright: © Neural Regeneration Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Yang, Yin Yang, Zhaoxi Lv, Maoxia Jia, Ang Li, Junjun Liao, Baitao Chen, Jing’an Wu, Zhengzheng Shi, Yi Xia, Yang Yao, Dezhong Chen, Ke Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice |
title | Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice |
title_full | Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice |
title_fullStr | Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice |
title_full_unstemmed | Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice |
title_short | Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice |
title_sort | morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (dba/2j) mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10479843/ https://www.ncbi.nlm.nih.gov/pubmed/37488870 http://dx.doi.org/10.4103/1673-5374.375341 |
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