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Programmable RNA detection with CRISPR-Cas12a

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Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that multiple orthologs of Cas12a activate trans-cleavage in the presence of split activators. Specifically, the PAM-distal regi...

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Detalles Bibliográficos
Autores principales:	Rananaware, Santosh R., Vesco, Emma K., Shoemaker, Grace M., Anekar, Swapnil S., Sandoval, Luke Samuel W., Meister, Katelyn S., Macaluso, Nicolas C., Nguyen, Long T., Jain, Piyush K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480431/ https://www.ncbi.nlm.nih.gov/pubmed/37669948 http://dx.doi.org/10.1038/s41467-023-41006-1

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