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Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing

Methicillin-resistant Staphylococcus aureus (MRSA) infection is a pressing clinical issue that impedes wound healing. Pro-inflammatory M1 macrophages is required to clear bacteria and recruit various cell types during the initial phase of wound healing, but timing of this process is crucial. Herein,...

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Autores principales: Xiao, Tianhua, Liu, Jiamin, Li, Yuanxin, Cai, Yu, Xing, Xudan, Shao, Ming, Zhang, Chi, Duan, Dongming, Liu, Song, Tan, Guoxin, Wang, Le, Wu, Zenghui, Gong, Zunlei, Zhou, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480781/
https://www.ncbi.nlm.nih.gov/pubmed/37680584
http://dx.doi.org/10.1016/j.mtbio.2023.100788
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author Xiao, Tianhua
Liu, Jiamin
Li, Yuanxin
Cai, Yu
Xing, Xudan
Shao, Ming
Zhang, Chi
Duan, Dongming
Liu, Song
Tan, Guoxin
Wang, Le
Wu, Zenghui
Gong, Zunlei
Zhou, Lei
author_facet Xiao, Tianhua
Liu, Jiamin
Li, Yuanxin
Cai, Yu
Xing, Xudan
Shao, Ming
Zhang, Chi
Duan, Dongming
Liu, Song
Tan, Guoxin
Wang, Le
Wu, Zenghui
Gong, Zunlei
Zhou, Lei
author_sort Xiao, Tianhua
collection PubMed
description Methicillin-resistant Staphylococcus aureus (MRSA) infection is a pressing clinical issue that impedes wound healing. Pro-inflammatory M1 macrophages is required to clear bacteria and recruit various cell types during the initial phase of wound healing, but timing of this process is crucial. Herein, a microenvironment-responsive nanofibrous dressing capable of timely macrophage phenotype transition in vivo is constructed by coating copper ions (Cu(2+))-polydopamine (PDA) networks on poly (ε-caprolactone) fiber (PCL-fiber) membrane. During the initial post-implantation period, the nanofibrous dressing show pH-sensitive Cu(2+) release in the acidic infection microenvironment. The release Cu(2+) have a direct killing effect on MRSA, and promote the proinflammatory M1 phenotype of macrophages to enhance the antibacterial macrophage response. Later, PDA to become a reactive oxygen species (ROS) scavenger when in microenvironments with elevated ROS levels, which conferred the dressing with an immunomodulatory activity that convert M1 macrophages into M2 macrophages. In vivo examination in an MRSA infected full-thickness skin wounds of rat model demonstrates that this dressing significantly facilitated infection eradication and wound healing through modulating local inflammatory phenotype. Overall, this study offers a simple and effective approach for timely manipulation of inflammation progression to promote infected wound healing.
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spelling pubmed-104807812023-09-07 Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing Xiao, Tianhua Liu, Jiamin Li, Yuanxin Cai, Yu Xing, Xudan Shao, Ming Zhang, Chi Duan, Dongming Liu, Song Tan, Guoxin Wang, Le Wu, Zenghui Gong, Zunlei Zhou, Lei Mater Today Bio Full Length Article Methicillin-resistant Staphylococcus aureus (MRSA) infection is a pressing clinical issue that impedes wound healing. Pro-inflammatory M1 macrophages is required to clear bacteria and recruit various cell types during the initial phase of wound healing, but timing of this process is crucial. Herein, a microenvironment-responsive nanofibrous dressing capable of timely macrophage phenotype transition in vivo is constructed by coating copper ions (Cu(2+))-polydopamine (PDA) networks on poly (ε-caprolactone) fiber (PCL-fiber) membrane. During the initial post-implantation period, the nanofibrous dressing show pH-sensitive Cu(2+) release in the acidic infection microenvironment. The release Cu(2+) have a direct killing effect on MRSA, and promote the proinflammatory M1 phenotype of macrophages to enhance the antibacterial macrophage response. Later, PDA to become a reactive oxygen species (ROS) scavenger when in microenvironments with elevated ROS levels, which conferred the dressing with an immunomodulatory activity that convert M1 macrophages into M2 macrophages. In vivo examination in an MRSA infected full-thickness skin wounds of rat model demonstrates that this dressing significantly facilitated infection eradication and wound healing through modulating local inflammatory phenotype. Overall, this study offers a simple and effective approach for timely manipulation of inflammation progression to promote infected wound healing. Elsevier 2023-09-01 /pmc/articles/PMC10480781/ /pubmed/37680584 http://dx.doi.org/10.1016/j.mtbio.2023.100788 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Xiao, Tianhua
Liu, Jiamin
Li, Yuanxin
Cai, Yu
Xing, Xudan
Shao, Ming
Zhang, Chi
Duan, Dongming
Liu, Song
Tan, Guoxin
Wang, Le
Wu, Zenghui
Gong, Zunlei
Zhou, Lei
Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing
title Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing
title_full Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing
title_fullStr Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing
title_full_unstemmed Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing
title_short Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing
title_sort microenvironment-responsive cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic mrsa infected wound healing
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480781/
https://www.ncbi.nlm.nih.gov/pubmed/37680584
http://dx.doi.org/10.1016/j.mtbio.2023.100788
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