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Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing
Methicillin-resistant Staphylococcus aureus (MRSA) infection is a pressing clinical issue that impedes wound healing. Pro-inflammatory M1 macrophages is required to clear bacteria and recruit various cell types during the initial phase of wound healing, but timing of this process is crucial. Herein,...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480781/ https://www.ncbi.nlm.nih.gov/pubmed/37680584 http://dx.doi.org/10.1016/j.mtbio.2023.100788 |
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author | Xiao, Tianhua Liu, Jiamin Li, Yuanxin Cai, Yu Xing, Xudan Shao, Ming Zhang, Chi Duan, Dongming Liu, Song Tan, Guoxin Wang, Le Wu, Zenghui Gong, Zunlei Zhou, Lei |
author_facet | Xiao, Tianhua Liu, Jiamin Li, Yuanxin Cai, Yu Xing, Xudan Shao, Ming Zhang, Chi Duan, Dongming Liu, Song Tan, Guoxin Wang, Le Wu, Zenghui Gong, Zunlei Zhou, Lei |
author_sort | Xiao, Tianhua |
collection | PubMed |
description | Methicillin-resistant Staphylococcus aureus (MRSA) infection is a pressing clinical issue that impedes wound healing. Pro-inflammatory M1 macrophages is required to clear bacteria and recruit various cell types during the initial phase of wound healing, but timing of this process is crucial. Herein, a microenvironment-responsive nanofibrous dressing capable of timely macrophage phenotype transition in vivo is constructed by coating copper ions (Cu(2+))-polydopamine (PDA) networks on poly (ε-caprolactone) fiber (PCL-fiber) membrane. During the initial post-implantation period, the nanofibrous dressing show pH-sensitive Cu(2+) release in the acidic infection microenvironment. The release Cu(2+) have a direct killing effect on MRSA, and promote the proinflammatory M1 phenotype of macrophages to enhance the antibacterial macrophage response. Later, PDA to become a reactive oxygen species (ROS) scavenger when in microenvironments with elevated ROS levels, which conferred the dressing with an immunomodulatory activity that convert M1 macrophages into M2 macrophages. In vivo examination in an MRSA infected full-thickness skin wounds of rat model demonstrates that this dressing significantly facilitated infection eradication and wound healing through modulating local inflammatory phenotype. Overall, this study offers a simple and effective approach for timely manipulation of inflammation progression to promote infected wound healing. |
format | Online Article Text |
id | pubmed-10480781 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104807812023-09-07 Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing Xiao, Tianhua Liu, Jiamin Li, Yuanxin Cai, Yu Xing, Xudan Shao, Ming Zhang, Chi Duan, Dongming Liu, Song Tan, Guoxin Wang, Le Wu, Zenghui Gong, Zunlei Zhou, Lei Mater Today Bio Full Length Article Methicillin-resistant Staphylococcus aureus (MRSA) infection is a pressing clinical issue that impedes wound healing. Pro-inflammatory M1 macrophages is required to clear bacteria and recruit various cell types during the initial phase of wound healing, but timing of this process is crucial. Herein, a microenvironment-responsive nanofibrous dressing capable of timely macrophage phenotype transition in vivo is constructed by coating copper ions (Cu(2+))-polydopamine (PDA) networks on poly (ε-caprolactone) fiber (PCL-fiber) membrane. During the initial post-implantation period, the nanofibrous dressing show pH-sensitive Cu(2+) release in the acidic infection microenvironment. The release Cu(2+) have a direct killing effect on MRSA, and promote the proinflammatory M1 phenotype of macrophages to enhance the antibacterial macrophage response. Later, PDA to become a reactive oxygen species (ROS) scavenger when in microenvironments with elevated ROS levels, which conferred the dressing with an immunomodulatory activity that convert M1 macrophages into M2 macrophages. In vivo examination in an MRSA infected full-thickness skin wounds of rat model demonstrates that this dressing significantly facilitated infection eradication and wound healing through modulating local inflammatory phenotype. Overall, this study offers a simple and effective approach for timely manipulation of inflammation progression to promote infected wound healing. Elsevier 2023-09-01 /pmc/articles/PMC10480781/ /pubmed/37680584 http://dx.doi.org/10.1016/j.mtbio.2023.100788 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Full Length Article Xiao, Tianhua Liu, Jiamin Li, Yuanxin Cai, Yu Xing, Xudan Shao, Ming Zhang, Chi Duan, Dongming Liu, Song Tan, Guoxin Wang, Le Wu, Zenghui Gong, Zunlei Zhou, Lei Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing |
title | Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing |
title_full | Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing |
title_fullStr | Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing |
title_full_unstemmed | Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing |
title_short | Microenvironment-responsive Cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic MRSA infected wound healing |
title_sort | microenvironment-responsive cu-phenolic networks coated nanofibrous dressing with timely macrophage phenotype transition for chronic mrsa infected wound healing |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10480781/ https://www.ncbi.nlm.nih.gov/pubmed/37680584 http://dx.doi.org/10.1016/j.mtbio.2023.100788 |
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