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TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus

Tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending on cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) cell resistance to influenza A virus (IVA) remains elusive. In the present study,...

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Autores principales: Le, Yang, Zhang, Jiayou, Gong, Zheng, Zhang, Zhegang, Nian, Xuanxuan, Li, Xuedan, Yu, Daiguan, Ma, Ning, Zhou, Rong, Zhang, Guomei, Liu, Bo, Yang, Lu, Fu, Baiqi, Xu, Xiuqin, Yang, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10481187/
https://www.ncbi.nlm.nih.gov/pubmed/37681145
http://dx.doi.org/10.1016/j.heliyon.2023.e19246
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author Le, Yang
Zhang, Jiayou
Gong, Zheng
Zhang, Zhegang
Nian, Xuanxuan
Li, Xuedan
Yu, Daiguan
Ma, Ning
Zhou, Rong
Zhang, Guomei
Liu, Bo
Yang, Lu
Fu, Baiqi
Xu, Xiuqin
Yang, Xiaoming
author_facet Le, Yang
Zhang, Jiayou
Gong, Zheng
Zhang, Zhegang
Nian, Xuanxuan
Li, Xuedan
Yu, Daiguan
Ma, Ning
Zhou, Rong
Zhang, Guomei
Liu, Bo
Yang, Lu
Fu, Baiqi
Xu, Xiuqin
Yang, Xiaoming
author_sort Le, Yang
collection PubMed
description Tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending on cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) cell resistance to influenza A virus (IVA) remains elusive. In the present study, CRISPR-Cas9 gene editing technology was used to construct the TRAF3 knockout MDCK cells (MDCK-TRAF3(−/−)). Hemagglutination assay, plaque assay, transcriptome, and quantitative real-time PCR were performed after IVA infection. The results showed that after IVA infection, HA titers and virus titers were promoted, interferon I-related pathways were significantly blocked, and transcription of several antiviral-related genes was significantly decreased in MDCK-TRAF3(−/−) cells. Thus, our study suggests that TRAF3 gene knockout reduced MDCK cell's resistance to IVA, thereby resulting in a promising way for IVA isolation and vaccine manufacturing.
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spelling pubmed-104811872023-09-07 TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus Le, Yang Zhang, Jiayou Gong, Zheng Zhang, Zhegang Nian, Xuanxuan Li, Xuedan Yu, Daiguan Ma, Ning Zhou, Rong Zhang, Guomei Liu, Bo Yang, Lu Fu, Baiqi Xu, Xiuqin Yang, Xiaoming Heliyon Research Article Tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending on cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) cell resistance to influenza A virus (IVA) remains elusive. In the present study, CRISPR-Cas9 gene editing technology was used to construct the TRAF3 knockout MDCK cells (MDCK-TRAF3(−/−)). Hemagglutination assay, plaque assay, transcriptome, and quantitative real-time PCR were performed after IVA infection. The results showed that after IVA infection, HA titers and virus titers were promoted, interferon I-related pathways were significantly blocked, and transcription of several antiviral-related genes was significantly decreased in MDCK-TRAF3(−/−) cells. Thus, our study suggests that TRAF3 gene knockout reduced MDCK cell's resistance to IVA, thereby resulting in a promising way for IVA isolation and vaccine manufacturing. Elsevier 2023-08-21 /pmc/articles/PMC10481187/ /pubmed/37681145 http://dx.doi.org/10.1016/j.heliyon.2023.e19246 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Le, Yang
Zhang, Jiayou
Gong, Zheng
Zhang, Zhegang
Nian, Xuanxuan
Li, Xuedan
Yu, Daiguan
Ma, Ning
Zhou, Rong
Zhang, Guomei
Liu, Bo
Yang, Lu
Fu, Baiqi
Xu, Xiuqin
Yang, Xiaoming
TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus
title TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus
title_full TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus
title_fullStr TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus
title_full_unstemmed TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus
title_short TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus
title_sort traf3 deficiency in mdck cells improved sensitivity to the influenza a virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10481187/
https://www.ncbi.nlm.nih.gov/pubmed/37681145
http://dx.doi.org/10.1016/j.heliyon.2023.e19246
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