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Evaluation of the diagnostic efficacy of EC-Test for latent tuberculosis infection in ambulatory people with HIV
BACKGROUND: Latent tuberculosis infection (LTBI) co-infected with human immunodeficiency virus (HIV) is more likely to develop into active tuberculosis (ATB), recombinant Mycobacterium tuberculosis fusion protein ESAT6/CFP10 (EC-Test) is a latest developed method for LTBI. Compared with the interfer...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10481920/ https://www.ncbi.nlm.nih.gov/pubmed/37074384 http://dx.doi.org/10.1097/QAD.0000000000003573 |
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author | Chongxing, Zhou Yuanchun, Li Yan, Huang Dabin, Liang Zhezhe, Cui Liwen, Huang Huifang, Qin Jing, Ye Fengxue, Long Xiaoyan, Liang Lifan, Zhang Xiaoqing, Liu Yanlin, Zhao Mei, Lin |
author_facet | Chongxing, Zhou Yuanchun, Li Yan, Huang Dabin, Liang Zhezhe, Cui Liwen, Huang Huifang, Qin Jing, Ye Fengxue, Long Xiaoyan, Liang Lifan, Zhang Xiaoqing, Liu Yanlin, Zhao Mei, Lin |
author_sort | Chongxing, Zhou |
collection | PubMed |
description | BACKGROUND: Latent tuberculosis infection (LTBI) co-infected with human immunodeficiency virus (HIV) is more likely to develop into active tuberculosis (ATB), recombinant Mycobacterium tuberculosis fusion protein ESAT6/CFP10 (EC-Test) is a latest developed method for LTBI. Compared with the interferon γ release test assays (IGRAs), the diagnostic performance of EC-Test to LTBI screening in HIV needs to be evaluated. METHODS: A population-based multicenter prospective study was conducted in Guangxi Province, China. The baseline data was collected and LTBI were measured by QuantiFERON-TB Gold In-Tube (QFT-GIT), EC-Test and T-cell spot of the TB assay (T-SPOT.TB). RESULTS: A total of 1478 patients were enrolled. when taking T-SPOT.TB as reference, the value of sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and consistency that EC-Test to diagnosis LTBI in HIV was 40.42, 97.98, 85.26, 85.04 and 85.06% respectively; when taking QFT-GIT as reference, the value was 36.00, 92.57, 55.10, 85.09 and 81.13%, respectively. When the CD4(+) cell count was <200 cells/μl, the accuracies of EC-Test to T-SPOT.TB and QFT-GIT were 87.12 and 88.89%, respectively; when it was 200 ≤ CD4(+) ≤ 500 cells/μl, the accuracies of EC-Test was 86.20 and 83.18%, respectively; when the CD4(+) cell count >500 cells/μl, the accuracies of EC-Test were 84.29 and 77.94%, respectively. The incidence of adverse reactions in EC-Test was 34.23% and the serious adverse reactions were 1.15%. CONCLUSION: EC-Test has good consistency compared with IGRAs in detecting LTBI in HIV no matter in different immunosuppression status or different regions, and the safety of EC-Test is also well, suitable for LTBI screening in HIV in high prevalence settings. |
format | Online Article Text |
id | pubmed-10481920 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-104819202023-09-07 Evaluation of the diagnostic efficacy of EC-Test for latent tuberculosis infection in ambulatory people with HIV Chongxing, Zhou Yuanchun, Li Yan, Huang Dabin, Liang Zhezhe, Cui Liwen, Huang Huifang, Qin Jing, Ye Fengxue, Long Xiaoyan, Liang Lifan, Zhang Xiaoqing, Liu Yanlin, Zhao Mei, Lin AIDS Clinical Science BACKGROUND: Latent tuberculosis infection (LTBI) co-infected with human immunodeficiency virus (HIV) is more likely to develop into active tuberculosis (ATB), recombinant Mycobacterium tuberculosis fusion protein ESAT6/CFP10 (EC-Test) is a latest developed method for LTBI. Compared with the interferon γ release test assays (IGRAs), the diagnostic performance of EC-Test to LTBI screening in HIV needs to be evaluated. METHODS: A population-based multicenter prospective study was conducted in Guangxi Province, China. The baseline data was collected and LTBI were measured by QuantiFERON-TB Gold In-Tube (QFT-GIT), EC-Test and T-cell spot of the TB assay (T-SPOT.TB). RESULTS: A total of 1478 patients were enrolled. when taking T-SPOT.TB as reference, the value of sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and consistency that EC-Test to diagnosis LTBI in HIV was 40.42, 97.98, 85.26, 85.04 and 85.06% respectively; when taking QFT-GIT as reference, the value was 36.00, 92.57, 55.10, 85.09 and 81.13%, respectively. When the CD4(+) cell count was <200 cells/μl, the accuracies of EC-Test to T-SPOT.TB and QFT-GIT were 87.12 and 88.89%, respectively; when it was 200 ≤ CD4(+) ≤ 500 cells/μl, the accuracies of EC-Test was 86.20 and 83.18%, respectively; when the CD4(+) cell count >500 cells/μl, the accuracies of EC-Test were 84.29 and 77.94%, respectively. The incidence of adverse reactions in EC-Test was 34.23% and the serious adverse reactions were 1.15%. CONCLUSION: EC-Test has good consistency compared with IGRAs in detecting LTBI in HIV no matter in different immunosuppression status or different regions, and the safety of EC-Test is also well, suitable for LTBI screening in HIV in high prevalence settings. Lippincott Williams & Wilkins 2023-10-01 2023-04-19 /pmc/articles/PMC10481920/ /pubmed/37074384 http://dx.doi.org/10.1097/QAD.0000000000003573 Text en Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Clinical Science Chongxing, Zhou Yuanchun, Li Yan, Huang Dabin, Liang Zhezhe, Cui Liwen, Huang Huifang, Qin Jing, Ye Fengxue, Long Xiaoyan, Liang Lifan, Zhang Xiaoqing, Liu Yanlin, Zhao Mei, Lin Evaluation of the diagnostic efficacy of EC-Test for latent tuberculosis infection in ambulatory people with HIV |
title | Evaluation of the diagnostic efficacy of EC-Test for latent tuberculosis infection in ambulatory people with HIV |
title_full | Evaluation of the diagnostic efficacy of EC-Test for latent tuberculosis infection in ambulatory people with HIV |
title_fullStr | Evaluation of the diagnostic efficacy of EC-Test for latent tuberculosis infection in ambulatory people with HIV |
title_full_unstemmed | Evaluation of the diagnostic efficacy of EC-Test for latent tuberculosis infection in ambulatory people with HIV |
title_short | Evaluation of the diagnostic efficacy of EC-Test for latent tuberculosis infection in ambulatory people with HIV |
title_sort | evaluation of the diagnostic efficacy of ec-test for latent tuberculosis infection in ambulatory people with hiv |
topic | Clinical Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10481920/ https://www.ncbi.nlm.nih.gov/pubmed/37074384 http://dx.doi.org/10.1097/QAD.0000000000003573 |
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