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Microfluidic delivery of cutting enzymes for fragmentation of surface-adsorbed DNA molecules

We describe a method for fragmenting, in-situ, surface-adsorbed and immobilized DNAs on polymethylmethacrylate(PMMA)-coated silicon substrates using microfluidic delivery of the cutting enzyme DNase I. Soft lithography is used to produce silicone elastomer (Sylgard 184) gratings which form microflui...

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Detalles Bibliográficos
Autores principales:	Budassi, Julia, Cho, NaHyun, Del Valle, Anthony, Sokolov, Jonathan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482287/ https://www.ncbi.nlm.nih.gov/pubmed/37672538 http://dx.doi.org/10.1371/journal.pone.0250054

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