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HIV-1 Vpr induces ciTRAN to prevent transcriptional repression of the provirus
The functional consequences of circular RNA (circRNA) expression on HIV-1 replication are largely unknown. Using a customized protocol involving direct RNA nanopore sequencing, here, we captured circRNAs from HIV-1–infected T cells and identified ciTRAN, a circRNA that modulates HIV-1 transcription....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482341/ https://www.ncbi.nlm.nih.gov/pubmed/37672576 http://dx.doi.org/10.1126/sciadv.adh9170 |
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author | Bhardwaj, Vipin Singh, Aman Choudhary, Aditi Dalavi, Rishikesh Ralte, Lalchhanhima Chawngthu, Richard L. Senthil Kumar, Nachimuthu Vijay, Nagarjun Chande, Ajit |
author_facet | Bhardwaj, Vipin Singh, Aman Choudhary, Aditi Dalavi, Rishikesh Ralte, Lalchhanhima Chawngthu, Richard L. Senthil Kumar, Nachimuthu Vijay, Nagarjun Chande, Ajit |
author_sort | Bhardwaj, Vipin |
collection | PubMed |
description | The functional consequences of circular RNA (circRNA) expression on HIV-1 replication are largely unknown. Using a customized protocol involving direct RNA nanopore sequencing, here, we captured circRNAs from HIV-1–infected T cells and identified ciTRAN, a circRNA that modulates HIV-1 transcription. We found that HIV-1 infection induces ciTRAN expression in a Vpr-dependent manner and that ciTRAN interacts with SRSF1, a protein known to repress HIV-1 transcription. Our results suggest that HIV-1 hijacks ciTRAN to exclude serine/arginine-rich splicing factor 1 (SRSF1) from the viral transcriptional complex, thereby promoting efficient viral transcription. In addition, we demonstrate that an SRSF1-inspired mimic can inhibit viral transcription regardless of ciTRAN induction. The hijacking of a host circRNA thus represents a previously unknown facet of primate lentiviruses in overcoming transmission bottlenecks. |
format | Online Article Text |
id | pubmed-10482341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104823412023-09-07 HIV-1 Vpr induces ciTRAN to prevent transcriptional repression of the provirus Bhardwaj, Vipin Singh, Aman Choudhary, Aditi Dalavi, Rishikesh Ralte, Lalchhanhima Chawngthu, Richard L. Senthil Kumar, Nachimuthu Vijay, Nagarjun Chande, Ajit Sci Adv Biomedicine and Life Sciences The functional consequences of circular RNA (circRNA) expression on HIV-1 replication are largely unknown. Using a customized protocol involving direct RNA nanopore sequencing, here, we captured circRNAs from HIV-1–infected T cells and identified ciTRAN, a circRNA that modulates HIV-1 transcription. We found that HIV-1 infection induces ciTRAN expression in a Vpr-dependent manner and that ciTRAN interacts with SRSF1, a protein known to repress HIV-1 transcription. Our results suggest that HIV-1 hijacks ciTRAN to exclude serine/arginine-rich splicing factor 1 (SRSF1) from the viral transcriptional complex, thereby promoting efficient viral transcription. In addition, we demonstrate that an SRSF1-inspired mimic can inhibit viral transcription regardless of ciTRAN induction. The hijacking of a host circRNA thus represents a previously unknown facet of primate lentiviruses in overcoming transmission bottlenecks. American Association for the Advancement of Science 2023-09-06 /pmc/articles/PMC10482341/ /pubmed/37672576 http://dx.doi.org/10.1126/sciadv.adh9170 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Bhardwaj, Vipin Singh, Aman Choudhary, Aditi Dalavi, Rishikesh Ralte, Lalchhanhima Chawngthu, Richard L. Senthil Kumar, Nachimuthu Vijay, Nagarjun Chande, Ajit HIV-1 Vpr induces ciTRAN to prevent transcriptional repression of the provirus |
title | HIV-1 Vpr induces ciTRAN to prevent transcriptional repression of the provirus |
title_full | HIV-1 Vpr induces ciTRAN to prevent transcriptional repression of the provirus |
title_fullStr | HIV-1 Vpr induces ciTRAN to prevent transcriptional repression of the provirus |
title_full_unstemmed | HIV-1 Vpr induces ciTRAN to prevent transcriptional repression of the provirus |
title_short | HIV-1 Vpr induces ciTRAN to prevent transcriptional repression of the provirus |
title_sort | hiv-1 vpr induces citran to prevent transcriptional repression of the provirus |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482341/ https://www.ncbi.nlm.nih.gov/pubmed/37672576 http://dx.doi.org/10.1126/sciadv.adh9170 |
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