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Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD
A major virulence trait of Mycobacterium tuberculosis (M. tb) is its ability to enter a dormant state within its human host. Since cell division is intimately linked to metabolic shut down, understanding the mechanism of septum formation and its integration with other events in the division pathway...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Microbiology Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482377/ https://www.ncbi.nlm.nih.gov/pubmed/37526955 http://dx.doi.org/10.1099/mic.0.001359 |
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author | Kishore, Vimal Gaiwala Sharma, Sujata S. Raghunand, Tirumalai R. |
author_facet | Kishore, Vimal Gaiwala Sharma, Sujata S. Raghunand, Tirumalai R. |
author_sort | Kishore, Vimal |
collection | PubMed |
description | A major virulence trait of Mycobacterium tuberculosis (M. tb) is its ability to enter a dormant state within its human host. Since cell division is intimately linked to metabolic shut down, understanding the mechanism of septum formation and its integration with other events in the division pathway is likely to offer clues to the molecular basis of dormancy. The M. tb genome lacks obvious homologues of several conserved cell division proteins, and this study was aimed at identifying and functionally characterising mycobacterial homologues of the E. coli septum site specification protein MinD (Ec MinD). Sequence homology based analyses suggested that the genomes of both M. tb and the saprophyte Mycobacterium smegmatis ( M. smegmatis ) encode two putative Ec MinD homologues - Rv1708/MSMEG_3743 and Rv3660c/ MSMEG_6171. Of these, Rv1708/MSMEG_3743 were found to be the true homologues, through complementation of the E. coli ∆minDE mutant HL1, overexpression studies, and structural comparisons. Rv1708 and MSMEG_3743 fully complemented the mini-cell phenotype of HL1, and over-expression of MSMEG_3743 in M. smegmatis led to cell elongation and a drastic decrease in c.f.u. counts, indicating its essentiality in cell-division. MSMEG_3743 displayed ATPase activity, consistent with its containing a conserved Walker A motif. Interaction of Rv1708 with the chromosome associated proteins ScpA and ParB, implied a link between its septum formation role, and chromosome segregation. Comparative structural analyses showed Rv1708 to be closer in similarity to Ec MinD than Rv3660c. In summary we identify Rv1708 and MSMEG_3743 to be homologues of Ec MinD, adding a critical missing piece to the mycobacterial cell division puzzle. |
format | Online Article Text |
id | pubmed-10482377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104823772023-09-07 Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD Kishore, Vimal Gaiwala Sharma, Sujata S. Raghunand, Tirumalai R. Microbiology (Reading) Microbial Physiology, Biochemistry and Metabolism A major virulence trait of Mycobacterium tuberculosis (M. tb) is its ability to enter a dormant state within its human host. Since cell division is intimately linked to metabolic shut down, understanding the mechanism of septum formation and its integration with other events in the division pathway is likely to offer clues to the molecular basis of dormancy. The M. tb genome lacks obvious homologues of several conserved cell division proteins, and this study was aimed at identifying and functionally characterising mycobacterial homologues of the E. coli septum site specification protein MinD (Ec MinD). Sequence homology based analyses suggested that the genomes of both M. tb and the saprophyte Mycobacterium smegmatis ( M. smegmatis ) encode two putative Ec MinD homologues - Rv1708/MSMEG_3743 and Rv3660c/ MSMEG_6171. Of these, Rv1708/MSMEG_3743 were found to be the true homologues, through complementation of the E. coli ∆minDE mutant HL1, overexpression studies, and structural comparisons. Rv1708 and MSMEG_3743 fully complemented the mini-cell phenotype of HL1, and over-expression of MSMEG_3743 in M. smegmatis led to cell elongation and a drastic decrease in c.f.u. counts, indicating its essentiality in cell-division. MSMEG_3743 displayed ATPase activity, consistent with its containing a conserved Walker A motif. Interaction of Rv1708 with the chromosome associated proteins ScpA and ParB, implied a link between its septum formation role, and chromosome segregation. Comparative structural analyses showed Rv1708 to be closer in similarity to Ec MinD than Rv3660c. In summary we identify Rv1708 and MSMEG_3743 to be homologues of Ec MinD, adding a critical missing piece to the mycobacterial cell division puzzle. Microbiology Society 2023-08-01 /pmc/articles/PMC10482377/ /pubmed/37526955 http://dx.doi.org/10.1099/mic.0.001359 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article. |
spellingShingle | Microbial Physiology, Biochemistry and Metabolism Kishore, Vimal Gaiwala Sharma, Sujata S. Raghunand, Tirumalai R. Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD |
title | Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD |
title_full | Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD |
title_fullStr | Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD |
title_full_unstemmed | Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD |
title_short | Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD |
title_sort | septum site placement in mycobacteria – identification and characterisation of mycobacterial homologues of escherichia coli mind |
topic | Microbial Physiology, Biochemistry and Metabolism |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482377/ https://www.ncbi.nlm.nih.gov/pubmed/37526955 http://dx.doi.org/10.1099/mic.0.001359 |
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