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Molecular Surface Quantification of Multifunctionalized Gold Nanoparticles Using UV–Visible Absorption Spectroscopy Deconvolution
[Image: see text] Multifunctional gold nanoparticles (AuNPs) are of great interest, owing to their vast potential for use in many areas including sensing, imaging, delivery, and medicine. A key factor in determining the biological activity of multifunctional AuNPs is the quantification of surface co...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10483462/ https://www.ncbi.nlm.nih.gov/pubmed/37621249 http://dx.doi.org/10.1021/acs.analchem.3c01649 |
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author | Potts, Jordan C. Jain, Akhil Amabilino, David B. Rawson, Frankie J. Pérez-García, Lluïsa |
author_facet | Potts, Jordan C. Jain, Akhil Amabilino, David B. Rawson, Frankie J. Pérez-García, Lluïsa |
author_sort | Potts, Jordan C. |
collection | PubMed |
description | [Image: see text] Multifunctional gold nanoparticles (AuNPs) are of great interest, owing to their vast potential for use in many areas including sensing, imaging, delivery, and medicine. A key factor in determining the biological activity of multifunctional AuNPs is the quantification of surface conjugated molecules. There has been a lack of accurate methods to determine this for multifunctionalized AuNPs. We address this limitation by using a new method based on the deconvolution and Levenberg–Marquardt algorithm fitting of UV–visible absorption spectrum to calculate the precise concentration and number of cytochrome C (Cyt C) and zinc porphyrin (Zn Porph) bound to each multifunctional AuNP. Dynamic light scattering (DLS) and zeta potential measurements were used to confirm the functionalization of AuNPs with Cyt C and Zn Porph. Transmission electron microscopy (TEM) was used in conjunction with UV–visible absorption spectroscopy and DLS to identify the AuNP size and confirm that no aggregation had taken place after functionalization. Despite the overlapping absorption bands of Cyt C and Zn Porph, this method was able to reveal a precise concentration and number of Cyt C and Zn Porph molecules attached per AuNP. Furthermore, using this method, we were able to identify unconjugated molecules, suggesting the need for further purification of the sample. This guide provides a simple and effective method to quickly quantify molecules bound to AuNPs, giving users valuable information, especially for applications in drug delivery and biosensors. |
format | Online Article Text |
id | pubmed-10483462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104834622023-09-08 Molecular Surface Quantification of Multifunctionalized Gold Nanoparticles Using UV–Visible Absorption Spectroscopy Deconvolution Potts, Jordan C. Jain, Akhil Amabilino, David B. Rawson, Frankie J. Pérez-García, Lluïsa Anal Chem [Image: see text] Multifunctional gold nanoparticles (AuNPs) are of great interest, owing to their vast potential for use in many areas including sensing, imaging, delivery, and medicine. A key factor in determining the biological activity of multifunctional AuNPs is the quantification of surface conjugated molecules. There has been a lack of accurate methods to determine this for multifunctionalized AuNPs. We address this limitation by using a new method based on the deconvolution and Levenberg–Marquardt algorithm fitting of UV–visible absorption spectrum to calculate the precise concentration and number of cytochrome C (Cyt C) and zinc porphyrin (Zn Porph) bound to each multifunctional AuNP. Dynamic light scattering (DLS) and zeta potential measurements were used to confirm the functionalization of AuNPs with Cyt C and Zn Porph. Transmission electron microscopy (TEM) was used in conjunction with UV–visible absorption spectroscopy and DLS to identify the AuNP size and confirm that no aggregation had taken place after functionalization. Despite the overlapping absorption bands of Cyt C and Zn Porph, this method was able to reveal a precise concentration and number of Cyt C and Zn Porph molecules attached per AuNP. Furthermore, using this method, we were able to identify unconjugated molecules, suggesting the need for further purification of the sample. This guide provides a simple and effective method to quickly quantify molecules bound to AuNPs, giving users valuable information, especially for applications in drug delivery and biosensors. American Chemical Society 2023-08-25 /pmc/articles/PMC10483462/ /pubmed/37621249 http://dx.doi.org/10.1021/acs.analchem.3c01649 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Potts, Jordan C. Jain, Akhil Amabilino, David B. Rawson, Frankie J. Pérez-García, Lluïsa Molecular Surface Quantification of Multifunctionalized Gold Nanoparticles Using UV–Visible Absorption Spectroscopy Deconvolution |
title | Molecular Surface
Quantification of Multifunctionalized
Gold Nanoparticles Using UV–Visible Absorption Spectroscopy
Deconvolution |
title_full | Molecular Surface
Quantification of Multifunctionalized
Gold Nanoparticles Using UV–Visible Absorption Spectroscopy
Deconvolution |
title_fullStr | Molecular Surface
Quantification of Multifunctionalized
Gold Nanoparticles Using UV–Visible Absorption Spectroscopy
Deconvolution |
title_full_unstemmed | Molecular Surface
Quantification of Multifunctionalized
Gold Nanoparticles Using UV–Visible Absorption Spectroscopy
Deconvolution |
title_short | Molecular Surface
Quantification of Multifunctionalized
Gold Nanoparticles Using UV–Visible Absorption Spectroscopy
Deconvolution |
title_sort | molecular surface
quantification of multifunctionalized
gold nanoparticles using uv–visible absorption spectroscopy
deconvolution |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10483462/ https://www.ncbi.nlm.nih.gov/pubmed/37621249 http://dx.doi.org/10.1021/acs.analchem.3c01649 |
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