Cargando…
Fluorescent Nanodiamonds for Tracking Single Polymer Particles in Cells and Tissues
[Image: see text] Polymer nanoparticles are widely used in drug delivery and are also a potential concern due to the increased burden of nano- or microplastics in the environment. In order to use polymer nanoparticles safely and understand their mechanism of action, it is useful to know where within...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10483464/ https://www.ncbi.nlm.nih.gov/pubmed/37612789 http://dx.doi.org/10.1021/acs.analchem.3c01452 |
_version_ | 1785102393535365120 |
---|---|
author | Li, Runrun Vedelaar, Thea A. Sigaeva, Alina Zhang, Yue Wu, Kaiqi Wang, Hui Wu, Xixi Olinga, Peter Wlodarzyk-Biegun, Małgorzata K. Schirhagl, Romana |
author_facet | Li, Runrun Vedelaar, Thea A. Sigaeva, Alina Zhang, Yue Wu, Kaiqi Wang, Hui Wu, Xixi Olinga, Peter Wlodarzyk-Biegun, Małgorzata K. Schirhagl, Romana |
author_sort | Li, Runrun |
collection | PubMed |
description | [Image: see text] Polymer nanoparticles are widely used in drug delivery and are also a potential concern due to the increased burden of nano- or microplastics in the environment. In order to use polymer nanoparticles safely and understand their mechanism of action, it is useful to know where within cells and tissues they end up. To this end, we labeled polymer nanoparticles with nanodiamond particles. More specifically, we have embedded nanodiamond particles in the polymer particles and characterized the composites. Compared to conventional fluorescent dyes, these labels have the advantage that nanodiamonds do not bleach or blink, thus allowing long-term imaging and tracking of polymer particles. We have demonstrated this principle both in cells and entire liver tissues. |
format | Online Article Text |
id | pubmed-10483464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-104834642023-09-08 Fluorescent Nanodiamonds for Tracking Single Polymer Particles in Cells and Tissues Li, Runrun Vedelaar, Thea A. Sigaeva, Alina Zhang, Yue Wu, Kaiqi Wang, Hui Wu, Xixi Olinga, Peter Wlodarzyk-Biegun, Małgorzata K. Schirhagl, Romana Anal Chem [Image: see text] Polymer nanoparticles are widely used in drug delivery and are also a potential concern due to the increased burden of nano- or microplastics in the environment. In order to use polymer nanoparticles safely and understand their mechanism of action, it is useful to know where within cells and tissues they end up. To this end, we labeled polymer nanoparticles with nanodiamond particles. More specifically, we have embedded nanodiamond particles in the polymer particles and characterized the composites. Compared to conventional fluorescent dyes, these labels have the advantage that nanodiamonds do not bleach or blink, thus allowing long-term imaging and tracking of polymer particles. We have demonstrated this principle both in cells and entire liver tissues. American Chemical Society 2023-08-23 /pmc/articles/PMC10483464/ /pubmed/37612789 http://dx.doi.org/10.1021/acs.analchem.3c01452 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Li, Runrun Vedelaar, Thea A. Sigaeva, Alina Zhang, Yue Wu, Kaiqi Wang, Hui Wu, Xixi Olinga, Peter Wlodarzyk-Biegun, Małgorzata K. Schirhagl, Romana Fluorescent Nanodiamonds for Tracking Single Polymer Particles in Cells and Tissues |
title | Fluorescent
Nanodiamonds for Tracking Single Polymer
Particles in Cells and Tissues |
title_full | Fluorescent
Nanodiamonds for Tracking Single Polymer
Particles in Cells and Tissues |
title_fullStr | Fluorescent
Nanodiamonds for Tracking Single Polymer
Particles in Cells and Tissues |
title_full_unstemmed | Fluorescent
Nanodiamonds for Tracking Single Polymer
Particles in Cells and Tissues |
title_short | Fluorescent
Nanodiamonds for Tracking Single Polymer
Particles in Cells and Tissues |
title_sort | fluorescent
nanodiamonds for tracking single polymer
particles in cells and tissues |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10483464/ https://www.ncbi.nlm.nih.gov/pubmed/37612789 http://dx.doi.org/10.1021/acs.analchem.3c01452 |
work_keys_str_mv | AT lirunrun fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT vedelaartheaa fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT sigaevaalina fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT zhangyue fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT wukaiqi fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT wanghui fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT wuxixi fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT olingapeter fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT wlodarzykbiegunmałgorzatak fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues AT schirhaglromana fluorescentnanodiamondsfortrackingsinglepolymerparticlesincellsandtissues |