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Saponins from Panax japonicus improve neuronal mitochondrial injury of aging rats
CONTEXT: Panax japonicus is the dried rhizome of Panax japonicus C.A. Mey. (Araliaceae). Saponins from Panax japonicus (SPJ) exhibit anti-oxidative and anti-aging effects. OBJECTIVE: We evaluated the neuroprotective effects of SPJ on aging rats. MATERIALS AND METHODS: Sprague-Dawley rats (18-months-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484050/ https://www.ncbi.nlm.nih.gov/pubmed/37667488 http://dx.doi.org/10.1080/13880209.2023.2244532 |
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author | Fan, Cheng Wang, Jin-Xin Xiong, Zhang-E. Hu, Shan-Shan Zhou, Ao-Jia Yuan, Ding Zhang, Chang-Cheng Zhou, Zhi-Yong Wang, Ting |
author_facet | Fan, Cheng Wang, Jin-Xin Xiong, Zhang-E. Hu, Shan-Shan Zhou, Ao-Jia Yuan, Ding Zhang, Chang-Cheng Zhou, Zhi-Yong Wang, Ting |
author_sort | Fan, Cheng |
collection | PubMed |
description | CONTEXT: Panax japonicus is the dried rhizome of Panax japonicus C.A. Mey. (Araliaceae). Saponins from Panax japonicus (SPJ) exhibit anti-oxidative and anti-aging effects. OBJECTIVE: We evaluated the neuroprotective effects of SPJ on aging rats. MATERIALS AND METHODS: Sprague-Dawley rats (18-months-old) were randomly divided into aging and SPJ groups (n = 8). Five-month-old rats were taken as the adult control (n = 8). The rats were fed a normal chow diet or the SPJ-containing diet (10 or 30 mg/kg) for 4 months. An in vitro model was established by d-galactose (d-Gal) in the SH-SY5Y cell line and pretreated with SPJ (25 and 50 µg/mL). The neuroprotection of SPJ was evaluated via Nissl staining, flow cytometry, transmission electron microscopy and Western blotting in vivo and in vitro. RESULTS: SPJ improved the neuronal degeneration and mitochondrial morphology that are associated with aging. Meanwhile, SPJ up-regulated the protein levels of mitofusin 2 (Mfn2) and optic atrophy 1 (Opa1) and down-regulated the protein level of dynamin-like protein 1 (Drp1) in the hippocampus of aging rats (p < 0.05 or p < 0.01 vs. 22 M). The in vitro studies also demonstrated that SPJ attenuated d-Gal-induced cell senescence concomitant with the improvement in mitochondrial function; SPJ, also up-regulated the Mfn2 and Opa1 protein levels, whereas the Drp1 protein level (p < 0.05 or p < 0.01 vs. d-Gal group) was down-regulated. DISCUSSION AND CONCLUSIONS: Further research on the elderly population will contribute to the development and utilization of SPJ for the treatment of neurodegenerative disorders. |
format | Online Article Text |
id | pubmed-10484050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-104840502023-09-08 Saponins from Panax japonicus improve neuronal mitochondrial injury of aging rats Fan, Cheng Wang, Jin-Xin Xiong, Zhang-E. Hu, Shan-Shan Zhou, Ao-Jia Yuan, Ding Zhang, Chang-Cheng Zhou, Zhi-Yong Wang, Ting Pharm Biol Research Article CONTEXT: Panax japonicus is the dried rhizome of Panax japonicus C.A. Mey. (Araliaceae). Saponins from Panax japonicus (SPJ) exhibit anti-oxidative and anti-aging effects. OBJECTIVE: We evaluated the neuroprotective effects of SPJ on aging rats. MATERIALS AND METHODS: Sprague-Dawley rats (18-months-old) were randomly divided into aging and SPJ groups (n = 8). Five-month-old rats were taken as the adult control (n = 8). The rats were fed a normal chow diet or the SPJ-containing diet (10 or 30 mg/kg) for 4 months. An in vitro model was established by d-galactose (d-Gal) in the SH-SY5Y cell line and pretreated with SPJ (25 and 50 µg/mL). The neuroprotection of SPJ was evaluated via Nissl staining, flow cytometry, transmission electron microscopy and Western blotting in vivo and in vitro. RESULTS: SPJ improved the neuronal degeneration and mitochondrial morphology that are associated with aging. Meanwhile, SPJ up-regulated the protein levels of mitofusin 2 (Mfn2) and optic atrophy 1 (Opa1) and down-regulated the protein level of dynamin-like protein 1 (Drp1) in the hippocampus of aging rats (p < 0.05 or p < 0.01 vs. 22 M). The in vitro studies also demonstrated that SPJ attenuated d-Gal-induced cell senescence concomitant with the improvement in mitochondrial function; SPJ, also up-regulated the Mfn2 and Opa1 protein levels, whereas the Drp1 protein level (p < 0.05 or p < 0.01 vs. d-Gal group) was down-regulated. DISCUSSION AND CONCLUSIONS: Further research on the elderly population will contribute to the development and utilization of SPJ for the treatment of neurodegenerative disorders. Taylor & Francis 2023-09-04 /pmc/articles/PMC10484050/ /pubmed/37667488 http://dx.doi.org/10.1080/13880209.2023.2244532 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Research Article Fan, Cheng Wang, Jin-Xin Xiong, Zhang-E. Hu, Shan-Shan Zhou, Ao-Jia Yuan, Ding Zhang, Chang-Cheng Zhou, Zhi-Yong Wang, Ting Saponins from Panax japonicus improve neuronal mitochondrial injury of aging rats |
title | Saponins from Panax japonicus improve neuronal mitochondrial injury of aging rats |
title_full | Saponins from Panax japonicus improve neuronal mitochondrial injury of aging rats |
title_fullStr | Saponins from Panax japonicus improve neuronal mitochondrial injury of aging rats |
title_full_unstemmed | Saponins from Panax japonicus improve neuronal mitochondrial injury of aging rats |
title_short | Saponins from Panax japonicus improve neuronal mitochondrial injury of aging rats |
title_sort | saponins from panax japonicus improve neuronal mitochondrial injury of aging rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484050/ https://www.ncbi.nlm.nih.gov/pubmed/37667488 http://dx.doi.org/10.1080/13880209.2023.2244532 |
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