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A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)

In the current study, we explored the relationship between melatonin and lactose synthesis in in vivo and in vitro conditions. We found that long-term melatonin feeding to the dairy cows significantly reduced the milk lactose content in a dose dependent manner. This lactose reduction was not associa...

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Autores principales: Liu, Yunjie, Yao, Songyang, Meng, Qinggeng, Liu, Xuening, Han, Huigang, Kan, Chunli, Wang, Tiankun, Wei, Wenjuan, Li, Shujing, Yu, Wenli, Zhao, Zengyuan, He, Changwang, Liu, Guoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484205/
https://www.ncbi.nlm.nih.gov/pubmed/37692118
http://dx.doi.org/10.7717/peerj.15932
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author Liu, Yunjie
Yao, Songyang
Meng, Qinggeng
Liu, Xuening
Han, Huigang
Kan, Chunli
Wang, Tiankun
Wei, Wenjuan
Li, Shujing
Yu, Wenli
Zhao, Zengyuan
He, Changwang
Liu, Guoshi
author_facet Liu, Yunjie
Yao, Songyang
Meng, Qinggeng
Liu, Xuening
Han, Huigang
Kan, Chunli
Wang, Tiankun
Wei, Wenjuan
Li, Shujing
Yu, Wenli
Zhao, Zengyuan
He, Changwang
Liu, Guoshi
author_sort Liu, Yunjie
collection PubMed
description In the current study, we explored the relationship between melatonin and lactose synthesis in in vivo and in vitro conditions. We found that long-term melatonin feeding to the dairy cows significantly reduced the milk lactose content in a dose dependent manner. This lactose reduction was not associated with a negative energy balance, since melatonin treatment did not alter the fat, glucose, or protein metabolisms of the cows. To identify the potential molecular mechanisms, the cow’s mammary epithelial cells were cultured for gene expression analysis. The results showed that the effect of melatonin on lactose reduction was mediated by its receptor MT1. MT1 activation downregulated the mRNA expression of the prolactin receptor gene (PRLR), which then suppressed the gene expression of SLC35B1. SLC35B1 is a galactose transporter and is responsible for the transportation of galactose to Golgi apparatus for lactose synthesis. Its suppression reduced the lactose synthesis and the milk lactose content. The discovery of this signal transduction pathway of melatonin on lactose synthesis provides a novel aspect of melatonin’s effect on carbohydrate metabolism in cows and maybe also in other mammals, including humans.
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spelling pubmed-104842052023-09-08 A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR) Liu, Yunjie Yao, Songyang Meng, Qinggeng Liu, Xuening Han, Huigang Kan, Chunli Wang, Tiankun Wei, Wenjuan Li, Shujing Yu, Wenli Zhao, Zengyuan He, Changwang Liu, Guoshi PeerJ Agricultural Science In the current study, we explored the relationship between melatonin and lactose synthesis in in vivo and in vitro conditions. We found that long-term melatonin feeding to the dairy cows significantly reduced the milk lactose content in a dose dependent manner. This lactose reduction was not associated with a negative energy balance, since melatonin treatment did not alter the fat, glucose, or protein metabolisms of the cows. To identify the potential molecular mechanisms, the cow’s mammary epithelial cells were cultured for gene expression analysis. The results showed that the effect of melatonin on lactose reduction was mediated by its receptor MT1. MT1 activation downregulated the mRNA expression of the prolactin receptor gene (PRLR), which then suppressed the gene expression of SLC35B1. SLC35B1 is a galactose transporter and is responsible for the transportation of galactose to Golgi apparatus for lactose synthesis. Its suppression reduced the lactose synthesis and the milk lactose content. The discovery of this signal transduction pathway of melatonin on lactose synthesis provides a novel aspect of melatonin’s effect on carbohydrate metabolism in cows and maybe also in other mammals, including humans. PeerJ Inc. 2023-09-04 /pmc/articles/PMC10484205/ /pubmed/37692118 http://dx.doi.org/10.7717/peerj.15932 Text en ©2023 Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Liu, Yunjie
Yao, Songyang
Meng, Qinggeng
Liu, Xuening
Han, Huigang
Kan, Chunli
Wang, Tiankun
Wei, Wenjuan
Li, Shujing
Yu, Wenli
Zhao, Zengyuan
He, Changwang
Liu, Guoshi
A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)
title A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)
title_full A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)
title_fullStr A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)
title_full_unstemmed A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)
title_short A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)
title_sort novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (mt1) and prolactin receptor (prlr)
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484205/
https://www.ncbi.nlm.nih.gov/pubmed/37692118
http://dx.doi.org/10.7717/peerj.15932
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