Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation

The neuronal activity in the respiratory network strongly depends on a variety of different neuromodulators. Given the essential role of astrocytes in stabilizing respiratory network activity generated by neurons in the preBötzinger complex (preBötC), our aim was to investigate astrocytic calcium si...

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Autores principales: Tacke, Charlotte, Bischoff, Anne M., Harb, Ali, Vafadari, Behnam, Hülsmann, Swen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Physiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484401/
https://www.ncbi.nlm.nih.gov/pubmed/37693007
http://dx.doi.org/10.3389/fphys.2023.1237376
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author Tacke, Charlotte
Bischoff, Anne M.
Harb, Ali
Vafadari, Behnam
Hülsmann, Swen
author_facet Tacke, Charlotte
Bischoff, Anne M.
Harb, Ali
Vafadari, Behnam
Hülsmann, Swen
author_sort Tacke, Charlotte
collection PubMed
description The neuronal activity in the respiratory network strongly depends on a variety of different neuromodulators. Given the essential role of astrocytes in stabilizing respiratory network activity generated by neurons in the preBötzinger complex (preBötC), our aim was to investigate astrocytic calcium signaling in the working heart brainstem preparation using fiber-optical imaging. By using transgenic mice that express GCaMP6s specifically in astrocytes, we successfully recorded astrocytic calcium signals in response to norepinephrine from individual astrocytes.
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spelling pubmed-104844012023-09-08 Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation Tacke, Charlotte Bischoff, Anne M. Harb, Ali Vafadari, Behnam Hülsmann, Swen Front Physiol Physiology The neuronal activity in the respiratory network strongly depends on a variety of different neuromodulators. Given the essential role of astrocytes in stabilizing respiratory network activity generated by neurons in the preBötzinger complex (preBötC), our aim was to investigate astrocytic calcium signaling in the working heart brainstem preparation using fiber-optical imaging. By using transgenic mice that express GCaMP6s specifically in astrocytes, we successfully recorded astrocytic calcium signals in response to norepinephrine from individual astrocytes. Frontiers Media S.A. 2023-08-24 /pmc/articles/PMC10484401/ /pubmed/37693007 http://dx.doi.org/10.3389/fphys.2023.1237376 Text en Copyright © 2023 Tacke, Bischoff, Harb, Vafadari and Hülsmann. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Tacke, Charlotte
Bischoff, Anne M.
Harb, Ali
Vafadari, Behnam
Hülsmann, Swen
Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation
title Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation
title_full Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation
title_fullStr Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation
title_full_unstemmed Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation
title_short Fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation
title_sort fiber optical imaging of astroglial calcium signaling in the respiratory network in the working heart brainstem preparation
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484401/
https://www.ncbi.nlm.nih.gov/pubmed/37693007
http://dx.doi.org/10.3389/fphys.2023.1237376
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