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Pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis
Extracellular vesicles (EVs) contain a variety of biomolecules and provide information about the cells that produce them. EVs from cancer cells found in urine can be used as biomarkers to detect cancer, enabling early diagnosis and treatment. The potential of alpha-2-macroglobulin (A2M) and clusteri...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484439/ https://www.ncbi.nlm.nih.gov/pubmed/37676879 http://dx.doi.org/10.1371/journal.pone.0291198 |
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author | Lee, Jisu Kim, Eunha Park, Joohee Choi, Seokjoo Lee, Myung-Shin Park, Jinsung |
author_facet | Lee, Jisu Kim, Eunha Park, Joohee Choi, Seokjoo Lee, Myung-Shin Park, Jinsung |
author_sort | Lee, Jisu |
collection | PubMed |
description | Extracellular vesicles (EVs) contain a variety of biomolecules and provide information about the cells that produce them. EVs from cancer cells found in urine can be used as biomarkers to detect cancer, enabling early diagnosis and treatment. The potential of alpha-2-macroglobulin (A2M) and clusterin (CLU) as novel diagnostic urinary EV (uEV) biomarkers for bladder cancer (BC) was demonstrated previously. To validate the diagnostic value of these proteins in uEVs in a large BC cohort, urine handling conditions before uEV isolation should be optimized during sample transportation from medical centers. In this study, we analyzed the uEV protein quantity, EV particle number, and uEV-A2M/CLU after urine storage at 20°C and 4°C for 0–6 days, each. A2M and CLU levels in uEVs were relatively stable when stored at 4°C for a maximum of three days and at 20°C for up to 24 h, with minimal impact on analysis results. Interestingly, pre-processing to remove debris and cells by centrifugation and filtration of urine did not show any beneficial effects on the preservation of protein biomarkers of uEVs during storage. Here, the importance of optimizing shipping conditions to minimize the impact of pre-analytical handling on the uEVs protein biomarkers was emphasized. These findings provide insights for the development of clinical protocols that use uEVs for diagnostic purposes. |
format | Online Article Text |
id | pubmed-10484439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104844392023-09-08 Pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis Lee, Jisu Kim, Eunha Park, Joohee Choi, Seokjoo Lee, Myung-Shin Park, Jinsung PLoS One Research Article Extracellular vesicles (EVs) contain a variety of biomolecules and provide information about the cells that produce them. EVs from cancer cells found in urine can be used as biomarkers to detect cancer, enabling early diagnosis and treatment. The potential of alpha-2-macroglobulin (A2M) and clusterin (CLU) as novel diagnostic urinary EV (uEV) biomarkers for bladder cancer (BC) was demonstrated previously. To validate the diagnostic value of these proteins in uEVs in a large BC cohort, urine handling conditions before uEV isolation should be optimized during sample transportation from medical centers. In this study, we analyzed the uEV protein quantity, EV particle number, and uEV-A2M/CLU after urine storage at 20°C and 4°C for 0–6 days, each. A2M and CLU levels in uEVs were relatively stable when stored at 4°C for a maximum of three days and at 20°C for up to 24 h, with minimal impact on analysis results. Interestingly, pre-processing to remove debris and cells by centrifugation and filtration of urine did not show any beneficial effects on the preservation of protein biomarkers of uEVs during storage. Here, the importance of optimizing shipping conditions to minimize the impact of pre-analytical handling on the uEVs protein biomarkers was emphasized. These findings provide insights for the development of clinical protocols that use uEVs for diagnostic purposes. Public Library of Science 2023-09-07 /pmc/articles/PMC10484439/ /pubmed/37676879 http://dx.doi.org/10.1371/journal.pone.0291198 Text en © 2023 Lee et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lee, Jisu Kim, Eunha Park, Joohee Choi, Seokjoo Lee, Myung-Shin Park, Jinsung Pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis |
title | Pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis |
title_full | Pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis |
title_fullStr | Pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis |
title_full_unstemmed | Pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis |
title_short | Pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis |
title_sort | pre-analytical handling conditions and protein marker recovery from urine extracellular vesicles for bladder cancer diagnosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484439/ https://www.ncbi.nlm.nih.gov/pubmed/37676879 http://dx.doi.org/10.1371/journal.pone.0291198 |
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