A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present...

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Detalles Bibliográficos
Autores principales: Ruiz Daniels, Rose, Taylor, Richard S., Dobie, Ross, Salisbury, Sarah, Furniss, James J., Clark, Emily, Macqueen, Daniel J., Robledo, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Lab Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484441/
https://www.ncbi.nlm.nih.gov/pubmed/37676875
http://dx.doi.org/10.1371/journal.pone.0285020
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author Ruiz Daniels, Rose
Taylor, Richard S.
Dobie, Ross
Salisbury, Sarah
Furniss, James J.
Clark, Emily
Macqueen, Daniel J.
Robledo, Diego
author_facet Ruiz Daniels, Rose
Taylor, Richard S.
Dobie, Ross
Salisbury, Sarah
Furniss, James J.
Clark, Emily
Macqueen, Daniel J.
Robledo, Diego
author_sort Ruiz Daniels, Rose
collection PubMed
description The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.
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spelling pubmed-104844412023-09-08 A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues Ruiz Daniels, Rose Taylor, Richard S. Dobie, Ross Salisbury, Sarah Furniss, James J. Clark, Emily Macqueen, Daniel J. Robledo, Diego PLoS One Lab Protocol The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species. Public Library of Science 2023-09-07 /pmc/articles/PMC10484441/ /pubmed/37676875 http://dx.doi.org/10.1371/journal.pone.0285020 Text en © 2023 Ruiz Daniels et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Lab Protocol
Ruiz Daniels, Rose
Taylor, Richard S.
Dobie, Ross
Salisbury, Sarah
Furniss, James J.
Clark, Emily
Macqueen, Daniel J.
Robledo, Diego
A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues
title A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues
title_full A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues
title_fullStr A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues
title_full_unstemmed A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues
title_short A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues
title_sort versatile nuclei extraction protocol for single nucleus sequencing in non-model species–optimization in various atlantic salmon tissues
topic Lab Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484441/
https://www.ncbi.nlm.nih.gov/pubmed/37676875
http://dx.doi.org/10.1371/journal.pone.0285020
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