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Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil
This study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, an...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484656/ https://www.ncbi.nlm.nih.gov/pubmed/37692921 http://dx.doi.org/10.1155/2023/3615757 |
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author | Shyaula, Manita Regmi, Sunil Khadka, Deegendra Poudel, Ram Chandra Dhakal, Agni Koirala, Devesh Sijapati, Jaishree Singh, Anjana Maharjan, Jyoti |
author_facet | Shyaula, Manita Regmi, Sunil Khadka, Deegendra Poudel, Ram Chandra Dhakal, Agni Koirala, Devesh Sijapati, Jaishree Singh, Anjana Maharjan, Jyoti |
author_sort | Shyaula, Manita |
collection | PubMed |
description | This study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, and molecular characterization identified PANG L as Bacillus licheniformis. This is the first study on the isolation of thermostable cellulase-producing Bacillus licheniformis from the Himalayan soil. This bacterium was processed for the production of cellulase enzyme. The optimum conditions for cellulase production were achieved at 45°C after 48 h of incubation at pH 6.5 in media-containing carboxymethyl cellulose (CMC) and yeast extract as carbon and nitrogen sources, respectively, in a thermo-shaker at 100 rpm. The enzyme was partially purified by 80% ammonium sulphate precipitation followed by dialysis, resulting in a 1.52-fold purification. The optimal activity of partially purified cellulase was observed at a temperature of 60°C and pH 5. The cellulase enzyme was stable within the pH ranges of 3–5 and retained 67% of activity even at 55°C. Cellulase activity was found to be enhanced in the presence of metal ions such as Cd(2+), Pb(2+), and Ba(2+). The enzyme showed the highest activity when CMC was used as a substrate, followed by cellobiose. The K(m) and V(max) values of the enzyme were 1.8 mg/ml and 10.92 μg/ml/min, respectively. The cellulase enzyme obtained from Bacillus licheniformis PANG L had suitable catalytic properties for use in industrial applications. |
format | Online Article Text |
id | pubmed-10484656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-104846562023-09-08 Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil Shyaula, Manita Regmi, Sunil Khadka, Deegendra Poudel, Ram Chandra Dhakal, Agni Koirala, Devesh Sijapati, Jaishree Singh, Anjana Maharjan, Jyoti Int J Microbiol Research Article This study aimed to isolate, purify, and characterize a potential thermophilic cellulase-producing bacterium from the Himalayan soil. Eleven thermophilic bacteria were isolated, and the strain PANG L was found to be the most potent cellulolytic producer. Morphological, physiological, biochemical, and molecular characterization identified PANG L as Bacillus licheniformis. This is the first study on the isolation of thermostable cellulase-producing Bacillus licheniformis from the Himalayan soil. This bacterium was processed for the production of cellulase enzyme. The optimum conditions for cellulase production were achieved at 45°C after 48 h of incubation at pH 6.5 in media-containing carboxymethyl cellulose (CMC) and yeast extract as carbon and nitrogen sources, respectively, in a thermo-shaker at 100 rpm. The enzyme was partially purified by 80% ammonium sulphate precipitation followed by dialysis, resulting in a 1.52-fold purification. The optimal activity of partially purified cellulase was observed at a temperature of 60°C and pH 5. The cellulase enzyme was stable within the pH ranges of 3–5 and retained 67% of activity even at 55°C. Cellulase activity was found to be enhanced in the presence of metal ions such as Cd(2+), Pb(2+), and Ba(2+). The enzyme showed the highest activity when CMC was used as a substrate, followed by cellobiose. The K(m) and V(max) values of the enzyme were 1.8 mg/ml and 10.92 μg/ml/min, respectively. The cellulase enzyme obtained from Bacillus licheniformis PANG L had suitable catalytic properties for use in industrial applications. Hindawi 2023-08-31 /pmc/articles/PMC10484656/ /pubmed/37692921 http://dx.doi.org/10.1155/2023/3615757 Text en Copyright © 2023 Manita Shyaula et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Shyaula, Manita Regmi, Sunil Khadka, Deegendra Poudel, Ram Chandra Dhakal, Agni Koirala, Devesh Sijapati, Jaishree Singh, Anjana Maharjan, Jyoti Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
title | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
title_full | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
title_fullStr | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
title_full_unstemmed | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
title_short | Characterization of Thermostable Cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil |
title_sort | characterization of thermostable cellulase from bacillus licheniformis pang l isolated from the himalayan soil |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484656/ https://www.ncbi.nlm.nih.gov/pubmed/37692921 http://dx.doi.org/10.1155/2023/3615757 |
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