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Click display: a rapid and efficient in vitro protein display method for directed evolution

We describe a novel method for in vitro protein display—click display—that does not depend on maintaining RNA integrity during biopanning and yields covalently linked protein–cDNA complexes from double-stranded input DNA within 2 h. The display is achieved in a one-pot format encompassing transcript...

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Detalles Bibliográficos
Autores principales:	Zeng, Yu, Woolley, Michael, Chockalingam, Karuppiah, Thomas, Benjamin, Arora, Srishtee, Hook, Magnus, Chen, Zhilei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484664/ https://www.ncbi.nlm.nih.gov/pubmed/37548398 http://dx.doi.org/10.1093/nar/gkad643

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