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A DNA unwinding equilibrium serves as a checkpoint for CRISPR-Cas12a target discrimination

CRISPR-associated proteins such as Cas9 and Cas12a are programable RNA-guided nucleases that have emerged as powerful tools for genome manipulation and molecular diagnostics. However, these enzymes are prone to cleaving off-target sequences that contain mismatches between the RNA guide and DNA proto...

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Detalles Bibliográficos
Autores principales:	Singh, Jaideep, Liu, Kevin G, Allen, Aleique, Jiang, Wei, Qin, Peter Z
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484686/ https://www.ncbi.nlm.nih.gov/pubmed/37522352 http://dx.doi.org/10.1093/nar/gkad636

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